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- W3176165736 abstract "A miniaturized optoelectronic sensor is demonstrated that measures total protein concentration in serum and urine with sensitivity and accuracy comparable to gold-standard methods. The sensor is comprised of a vertical cavity surface emitting laser (VCSEL), photodetector and other custom optical components and electronics that can be hybrid packaged into a portable, handheld form factor. In conjunction, a custom fluorescence assay has been developed based on the protein-induced fluorescence enhancement (PIFE) phenomenon, enabling real-time sensor response to changes in protein concentration. Methods are described for the following: Standard curves: Used to determine the sensitivity, dynamic range, and linearity of the VCSEL biosensor/PIFE assay system in buffer as well as in human blood and urine samples. Comparison of VCSEL biosensor performance with a benchtop fluorimetric microplate reader. Accuracy of the VCSEL biosensor/PIFE assay system: Evaluated by comparing sensor measurements with gold-standard clinical laboratory measurements of total protein in serum and urine samples from patients with diabetes." @default.
- W3176165736 created "2021-07-05" @default.
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- W3176165736 date "2021-01-01" @default.
- W3176165736 modified "2023-09-23" @default.
- W3176165736 title "A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis" @default.
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- W3176165736 doi "https://doi.org/10.1016/j.mex.2021.101414" @default.
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