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• W3176201391 startingPage "101365" @default.
• W3176201391 abstract "Virus injection into EGK-X embryos is a well-defined approach in avian transgenesis. This system uses a chicken ovalbumin gene promoter to induce transgene expression in the chicken oviduct. Although a reconstructed chicken ovalbumin promoter that links an ovalbumin promoter and estrogen-responsive enhancer element ( ERE ) is useful, a large viral vector containing the ovalbumin promoter and a target gene restricts viral packaging capacity and produces low-titer virus particles. We newly developed recombinant chicken promoters by linking regulatory regions of ovalbumin and other oviduct-specific genes. Putative enhancer fragments of the genes, such as ovotransferrin ( TF ), ovomucin alpha subunit ( OVOA ) , and ovalbumin-related protein X ( OVALX ), were placed at the 5`-flanking region of the 2.8-kb ovalbumin promoter. Basal promoter fragments of the genes, namely, pTF, lysozyme (p LYZ ) , and ovomucoid (p OVM ), were placed at the 3`-flanking region of the 1.6-kb ovalbumin ERE. The recombinant promoters cloned into each reporter vector were evaluated using a dual luciferase assay in human and chicken somatic cells, and LMH/2A cells treated with 0-1,000 nM estrogen, and cultured primary chicken oviduct cells. The recombinant promoters with linking ovalbumin and TF, OVOA, pOVM, and pLYZ regulatory regions had 2.1- to 19.5-fold ( P < 0.05) higher luciferase activity than the reconstructed ovalbumin promoter in chicken oviduct cells. Therefore, recombinant promoters may be used to efficiently drive transgene expression in transgenic chickens." @default.
• W3176201391 created "2021-07-05" @default.
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• W3176201391 date "2021-10-01" @default.
• W3176201391 modified "2023-09-24" @default.
• W3176201391 title "Development and in vitro evaluation of recombinant chicken promoters to efficiently drive transgene expression in chicken oviduct cells" @default.
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• W3176201391 doi "https://doi.org/10.1016/j.psj.2021.101365" @default.
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