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- W3176247108 abstract "Abstract Forces exerted at the molecular level are central to countless processes in health and disease. However, measuring molecular forces remains difficult if not impossible using existing approaches. During clathrin-mediated endocytosis (CME), the plasma membrane is deformed into a vesicle by the forces produced by the actin cytoskeleton and transmitted to the membrane by a multi-protein coat 1–3 . However, the actual forces required for endocytosis remain unknown. Here we present a new series of in vivo force sensors to measure the forces on the fission yeast HIP1R homologue End4p, a protein that links the endocytic membrane to the actin cytoskeleton 2,4 . These new force sensors are based on calibrated coiled-coils that phase separate when they are under force. The measured forces on End4p are between 11 and 20 pN near the actin meshwork, between 10 and 11 pN near the clathrin lattice, and between 8 and 10 pN near the plasma membrane. Our results predict the participation of additional proteins to relay forces in different layers of the endocytic machinery during CME, and our approach points to a novel direction for in vivo force measurement." @default.
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- W3176247108 date "2021-06-30" @default.
- W3176247108 modified "2023-09-24" @default.
- W3176247108 title "Force redistribution during clathrin-mediated endocytosis revealed by new phase-separating force sensors" @default.
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