Matches in SemOpenAlex for { <https://semopenalex.org/work/W3176322012> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W3176322012 abstract "Pituitary adenylate cyclase-activating polypeptide (PACAP) but not vasoactive intestinal peptide (VIP) injected into the common carotid artery stimulates ventilation, suggesting activation of PAC1 receptors within the primary peripheral respiratory chemoreceptor, the carotid body (CB). Possible biochemical pathways downstream of the PAC1 receptor include G-proteins activation of (a) PLC which stimulates protein kinase C (PKC) and (b) cAMP which stimulates protein kinase A (PKA). Here we elucidate the relative importance of PKC and PKA in mediating PACAP's effects on carotid sinus nerve activity. We used an ex-vivo artificially – perfused rat CB preparation and measured integrated CSN activity during normoxia (PO2 ≃ 100 Torr/PCO2 ≃ 35 Torr) and tested effects of PACAP 1–38 (100 nM) alone and against a background of : (a) PKC inhibitors – GF 109203X (GF;10 μM) and Chelerythrine chloride (CC;20 μM) and (b) PKA inhibitor – H89 (10 μM). We demonstrate PACAP stimulates CSN activity in a biphasic manner, with an initial transient stimulatory phase, followed by a steady-state phase. PKC inhibitors, GF and CC (n=6) reduced both peak and steady state PACAP responses by over 50%. The PKA inhibitor H89 had no effect on the transient phase but reduced the steady state by 17%. In conclusion, PACAP induced stimulation of CSN activity is mediated primarily through a PLC-IP3 - PKC, not a cAMP-PKA pathway." @default.
- W3176322012 created "2021-07-05" @default.
- W3176322012 creator A5043722164 @default.
- W3176322012 creator A5051519736 @default.
- W3176322012 date "2012-04-01" @default.
- W3176322012 modified "2023-09-26" @default.
- W3176322012 title "Carotid body afferent activity stimulation by PACAP is mediated through PKC pathway" @default.
- W3176322012 doi "https://doi.org/10.1096/fasebj.26.1_supplement.897.7" @default.
- W3176322012 hasPublicationYear "2012" @default.
- W3176322012 type Work @default.
- W3176322012 sameAs 3176322012 @default.
- W3176322012 citedByCount "0" @default.
- W3176322012 crossrefType "journal-article" @default.
- W3176322012 hasAuthorship W3176322012A5043722164 @default.
- W3176322012 hasAuthorship W3176322012A5051519736 @default.
- W3176322012 hasConcept C118303440 @default.
- W3176322012 hasConcept C126322002 @default.
- W3176322012 hasConcept C134018914 @default.
- W3176322012 hasConcept C170493617 @default.
- W3176322012 hasConcept C180526460 @default.
- W3176322012 hasConcept C184235292 @default.
- W3176322012 hasConcept C185592680 @default.
- W3176322012 hasConcept C195794163 @default.
- W3176322012 hasConcept C24998067 @default.
- W3176322012 hasConcept C2776108454 @default.
- W3176322012 hasConcept C2778113364 @default.
- W3176322012 hasConcept C2779496938 @default.
- W3176322012 hasConcept C2780069284 @default.
- W3176322012 hasConcept C55493867 @default.
- W3176322012 hasConcept C62478195 @default.
- W3176322012 hasConcept C71924100 @default.
- W3176322012 hasConcept C8330315 @default.
- W3176322012 hasConcept C86803240 @default.
- W3176322012 hasConcept C97029542 @default.
- W3176322012 hasConceptScore W3176322012C118303440 @default.
- W3176322012 hasConceptScore W3176322012C126322002 @default.
- W3176322012 hasConceptScore W3176322012C134018914 @default.
- W3176322012 hasConceptScore W3176322012C170493617 @default.
- W3176322012 hasConceptScore W3176322012C180526460 @default.
- W3176322012 hasConceptScore W3176322012C184235292 @default.
- W3176322012 hasConceptScore W3176322012C185592680 @default.
- W3176322012 hasConceptScore W3176322012C195794163 @default.
- W3176322012 hasConceptScore W3176322012C24998067 @default.
- W3176322012 hasConceptScore W3176322012C2776108454 @default.
- W3176322012 hasConceptScore W3176322012C2778113364 @default.
- W3176322012 hasConceptScore W3176322012C2779496938 @default.
- W3176322012 hasConceptScore W3176322012C2780069284 @default.
- W3176322012 hasConceptScore W3176322012C55493867 @default.
- W3176322012 hasConceptScore W3176322012C62478195 @default.
- W3176322012 hasConceptScore W3176322012C71924100 @default.
- W3176322012 hasConceptScore W3176322012C8330315 @default.
- W3176322012 hasConceptScore W3176322012C86803240 @default.
- W3176322012 hasConceptScore W3176322012C97029542 @default.
- W3176322012 hasIssue "S1" @default.
- W3176322012 hasLocation W31763220121 @default.
- W3176322012 hasOpenAccess W3176322012 @default.
- W3176322012 hasPrimaryLocation W31763220121 @default.
- W3176322012 hasRelatedWork W1577371918 @default.
- W3176322012 hasRelatedWork W1967851866 @default.
- W3176322012 hasRelatedWork W2000223190 @default.
- W3176322012 hasRelatedWork W2025414496 @default.
- W3176322012 hasRelatedWork W2029015456 @default.
- W3176322012 hasRelatedWork W2045039130 @default.
- W3176322012 hasRelatedWork W2093528009 @default.
- W3176322012 hasRelatedWork W2101674665 @default.
- W3176322012 hasRelatedWork W2365328411 @default.
- W3176322012 hasRelatedWork W2467624521 @default.
- W3176322012 hasVolume "26" @default.
- W3176322012 isParatext "false" @default.
- W3176322012 isRetracted "false" @default.
- W3176322012 magId "3176322012" @default.
- W3176322012 workType "article" @default.