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• W3176575851 abstract "Abstract Nanopore sequencing is an emerging genomic technology with great potential. However, the storage and analysis of nanopore sequencing data have become major bottlenecks preventing more widespread adoption in research and clinical genomics. Here, we elucidate an inherent limitation in the file format used to store raw nanopore data – known as FAST5 – that prevents efficient analysis on high-performance computing (HPC) systems. To overcome this we have developed SLOW5, an alternative file format that permits efficient parallelisation and, thereby, acceleration of nanopore data analysis. For example, we show that using SLOW5 format, instead of FAST5, reduces the time and cost of genome-wide DNA methylation profiling by an order of magnitude on common HPC systems, and delivers consistent improvements on a wide range of different architectures. With a simple, accessible file structure and a ~25% reduction in size compared to FAST5, SLOW5 format will deliver substantial benefits to all areas of the nanopore community." @default.
• W3176575851 created "2021-07-05" @default.
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• W3176575851 date "2021-07-13" @default.
• W3176575851 modified "2023-10-05" @default.
• W3176575851 title "SLOW5: a new file format enables massive acceleration of nanopore sequencing data analysis" @default.
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• W3176575851 doi "https://doi.org/10.21203/rs.3.rs-668517/v1" @default.
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