Matches in SemOpenAlex for { <https://semopenalex.org/work/W3176800219> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W3176800219 abstract "Liposomes have been used as a pseudo cell membrane for encapsulating biomolecules and creating an artificial cell in the interior where biochemical reactions can occur. Among the several methods used to prepare biomolecule-encapsulating liposomes, the spontaneous emulsion transfer method is superior to others in that it allows us to readily prepare relatively large liposomes whose sizes are controlled (from micrometer- to millimeter-sized liposomes) without special equipment. However, conventional protocols for this method require liposomes to contain a considerably high concentration of sucrose (high-density solute), which severely inhibits gene expression, one of the most important biochemical reactions. Thus, we optimized the preparation conditions to develop a wheat germ extract (WGE)-based protocol that requires a much lower concentration of sucrose and has almost no effect on eukaryotic cell-free translation. Our protocol allows us to successfully prepare millimeter-sized, moderately stable, WGE-encapsulating liposomes in which WGE translation takes place efficiently. Since a broad range of genes derived from various types of organisms can be efficiently translated in a WGE-based translation system, liposomes prepared using our protocol would be useful as a versatile research tool for artificial cells." @default.
- W3176800219 created "2021-07-05" @default.
- W3176800219 creator A5007428793 @default.
- W3176800219 creator A5076043734 @default.
- W3176800219 date "2021-01-01" @default.
- W3176800219 modified "2023-10-18" @default.
- W3176800219 title "A Detailed Protocol for Preparing Millimeter-sized Supergiant Liposomes that Permit Efficient Eukaryotic Cell-free Translation in the Interior" @default.
- W3176800219 cites W1963611162 @default.
- W3176800219 cites W1967139808 @default.
- W3176800219 cites W1982747344 @default.
- W3176800219 cites W1997763444 @default.
- W3176800219 cites W2057319782 @default.
- W3176800219 cites W2083288707 @default.
- W3176800219 cites W2130531386 @default.
- W3176800219 cites W2146631896 @default.
- W3176800219 cites W2160121579 @default.
- W3176800219 cites W2469313943 @default.
- W3176800219 cites W2912770194 @default.
- W3176800219 cites W3036739247 @default.
- W3176800219 doi "https://doi.org/10.21769/bioprotoc.4054" @default.
- W3176800219 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8260257" @default.
- W3176800219 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34262997" @default.
- W3176800219 hasPublicationYear "2021" @default.
- W3176800219 type Work @default.
- W3176800219 sameAs 3176800219 @default.
- W3176800219 citedByCount "1" @default.
- W3176800219 countsByYear W31768002192021 @default.
- W3176800219 crossrefType "journal-article" @default.
- W3176800219 hasAuthorship W3176800219A5007428793 @default.
- W3176800219 hasAuthorship W3176800219A5076043734 @default.
- W3176800219 hasBestOaLocation W31768002191 @default.
- W3176800219 hasConcept C104317684 @default.
- W3176800219 hasConcept C105580179 @default.
- W3176800219 hasConcept C149364088 @default.
- W3176800219 hasConcept C171250308 @default.
- W3176800219 hasConcept C185154212 @default.
- W3176800219 hasConcept C185592680 @default.
- W3176800219 hasConcept C192562407 @default.
- W3176800219 hasConcept C3020307904 @default.
- W3176800219 hasConcept C41008148 @default.
- W3176800219 hasConcept C49853544 @default.
- W3176800219 hasConcept C55493867 @default.
- W3176800219 hasConceptScore W3176800219C104317684 @default.
- W3176800219 hasConceptScore W3176800219C105580179 @default.
- W3176800219 hasConceptScore W3176800219C149364088 @default.
- W3176800219 hasConceptScore W3176800219C171250308 @default.
- W3176800219 hasConceptScore W3176800219C185154212 @default.
- W3176800219 hasConceptScore W3176800219C185592680 @default.
- W3176800219 hasConceptScore W3176800219C192562407 @default.
- W3176800219 hasConceptScore W3176800219C3020307904 @default.
- W3176800219 hasConceptScore W3176800219C41008148 @default.
- W3176800219 hasConceptScore W3176800219C49853544 @default.
- W3176800219 hasConceptScore W3176800219C55493867 @default.
- W3176800219 hasIssue "12" @default.
- W3176800219 hasLocation W31768002191 @default.
- W3176800219 hasLocation W31768002192 @default.
- W3176800219 hasLocation W31768002193 @default.
- W3176800219 hasOpenAccess W3176800219 @default.
- W3176800219 hasPrimaryLocation W31768002191 @default.
- W3176800219 hasRelatedWork W1970136400 @default.
- W3176800219 hasRelatedWork W2058527827 @default.
- W3176800219 hasRelatedWork W2393139867 @default.
- W3176800219 hasRelatedWork W2553322681 @default.
- W3176800219 hasRelatedWork W2742420766 @default.
- W3176800219 hasRelatedWork W2748952813 @default.
- W3176800219 hasRelatedWork W2891622084 @default.
- W3176800219 hasRelatedWork W2899084033 @default.
- W3176800219 hasRelatedWork W2914170288 @default.
- W3176800219 hasRelatedWork W2982595580 @default.
- W3176800219 hasVolume "11" @default.
- W3176800219 isParatext "false" @default.
- W3176800219 isRetracted "false" @default.
- W3176800219 magId "3176800219" @default.
- W3176800219 workType "article" @default.