Matches in SemOpenAlex for { <https://semopenalex.org/work/W3176821735> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W3176821735 abstract "In efforts to identify cellular mechanisms involved in differences among inbred rat strains in survival time after severe hemorrhage (STaH) (Klemcke et al. Physiol Genomics 43: 758, 2011), we evaluated HOA, a cytoprotective enzyme that catalyzes conversion of cellular heme into biliverdin, free iron (Fe+2), and carbon monoxide. We adapted a HOA procedure used with purified HO (Reed et al, DMD 38: 2060, 2010) for use in tissues (liver microsomal fraction); the assay measures the appearance of Fe+2 using hemin substrate and NADPH as cofactor. HOA had an apparent Km of 7.8 μM of hemin; Vmax= 170 nM Fe+2 /mg protein/min; time period of initial velocity of 0 to 40 min; and a linearity of Fe+2 formation for 0.25 to 3 mg of microsomal protein. Dark Agouti (DA) rats (longest STaH) were assigned to one of three treatments (n=5/ treatment): non-operated controls, catheterized (CATH) only, or CATH plus hemorrhage (49% of blood volume) 24 h post-surgery. Rats were euthanized 30 min after handling or after the start of the hemorrhage. HOA increased 32% 24 hrs after CATH alone (4.69 vs 3.54 nmol Fe+2/mg protein/30 min; P = 0.05). HOA increased 36% further after hemorrhage to 6.37 nmol Fe+2/mg protein/30 min (P = 0.01). Hence in liver of DA rats, HOA is increased and may be exerting a cytoprotective effect after stressors such as surgery and hemorrhage. Moreover, this assay allows for an easier, more direct procedure for measuring HOA in tissues." @default.
- W3176821735 created "2021-07-05" @default.
- W3176821735 creator A5010030117 @default.
- W3176821735 creator A5012712307 @default.
- W3176821735 date "2012-04-01" @default.
- W3176821735 modified "2023-10-16" @default.
- W3176821735 title "Heme oxygenase activity (HOA) in rat liver: Response to severe hemorrhage in an inbred rat strain" @default.
- W3176821735 doi "https://doi.org/10.1096/fasebj.26.1_supplement.lb772" @default.
- W3176821735 hasPublicationYear "2012" @default.
- W3176821735 type Work @default.
- W3176821735 sameAs 3176821735 @default.
- W3176821735 citedByCount "0" @default.
- W3176821735 crossrefType "journal-article" @default.
- W3176821735 hasAuthorship W3176821735A5010030117 @default.
- W3176821735 hasAuthorship W3176821735A5012712307 @default.
- W3176821735 hasConcept C126322002 @default.
- W3176821735 hasConcept C134018914 @default.
- W3176821735 hasConcept C153911025 @default.
- W3176821735 hasConcept C16685009 @default.
- W3176821735 hasConcept C181199279 @default.
- W3176821735 hasConcept C185592680 @default.
- W3176821735 hasConcept C20705724 @default.
- W3176821735 hasConcept C2776217839 @default.
- W3176821735 hasConcept C2776253728 @default.
- W3176821735 hasConcept C2777723881 @default.
- W3176821735 hasConcept C2778022156 @default.
- W3176821735 hasConcept C2779219270 @default.
- W3176821735 hasConcept C55493867 @default.
- W3176821735 hasConcept C71924100 @default.
- W3176821735 hasConcept C86803240 @default.
- W3176821735 hasConcept C87644729 @default.
- W3176821735 hasConceptScore W3176821735C126322002 @default.
- W3176821735 hasConceptScore W3176821735C134018914 @default.
- W3176821735 hasConceptScore W3176821735C153911025 @default.
- W3176821735 hasConceptScore W3176821735C16685009 @default.
- W3176821735 hasConceptScore W3176821735C181199279 @default.
- W3176821735 hasConceptScore W3176821735C185592680 @default.
- W3176821735 hasConceptScore W3176821735C20705724 @default.
- W3176821735 hasConceptScore W3176821735C2776217839 @default.
- W3176821735 hasConceptScore W3176821735C2776253728 @default.
- W3176821735 hasConceptScore W3176821735C2777723881 @default.
- W3176821735 hasConceptScore W3176821735C2778022156 @default.
- W3176821735 hasConceptScore W3176821735C2779219270 @default.
- W3176821735 hasConceptScore W3176821735C55493867 @default.
- W3176821735 hasConceptScore W3176821735C71924100 @default.
- W3176821735 hasConceptScore W3176821735C86803240 @default.
- W3176821735 hasConceptScore W3176821735C87644729 @default.
- W3176821735 hasIssue "S1" @default.
- W3176821735 hasLocation W31768217351 @default.
- W3176821735 hasOpenAccess W3176821735 @default.
- W3176821735 hasPrimaryLocation W31768217351 @default.
- W3176821735 hasRelatedWork W1488164411 @default.
- W3176821735 hasRelatedWork W1588362559 @default.
- W3176821735 hasRelatedWork W1909602766 @default.
- W3176821735 hasRelatedWork W2019209742 @default.
- W3176821735 hasRelatedWork W2021557265 @default.
- W3176821735 hasRelatedWork W2030908512 @default.
- W3176821735 hasRelatedWork W2044670036 @default.
- W3176821735 hasRelatedWork W2055317690 @default.
- W3176821735 hasRelatedWork W2162202064 @default.
- W3176821735 hasRelatedWork W2279101380 @default.
- W3176821735 hasVolume "26" @default.
- W3176821735 isParatext "false" @default.
- W3176821735 isRetracted "false" @default.
- W3176821735 magId "3176821735" @default.
- W3176821735 workType "article" @default.