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- W3176854311 abstract "CRISPR-based technologies have revolutionized biochemical and biomedical research. In the past few years, many CRISPR systems have been discovered and characterized. These CRISPR systems greatly expand the CRISPR toolkit. Cas13b is the most recently identified CRISPR system. The usefulness of the Cas13b system as a gene editing tool has been demonstrated in human cells. Cas13b system can be exploited to achieve not only RNA knockdown, but also highly specific and efficient RNA editing. The RNA editing ability of the Cas13b-based technology has been used for the correction of human disease-relevant mutations. Despite the rapid progress of Cas13b research, little is known about the structures and catalytic mechanisms of this important family of CRISPR enzymes. A cross-disciplinary research approach is employed in our study to characterize the two CRISPR-Cas13b systems in Porphyromonas gingivalis (Pgi5 and Pgi8 Cas13b systems). Both Cas13b enzymes possess dual RNase activities for pre-crRNA processing (to produce the mature crRNAs) and target RNA cleavage. The systems are capable of mRNA knockdown in human cells. Support or Funding Information 1. SIUC Seed Grant 2. NIH Foundation This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal." @default.
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- W3176854311 date "2019-04-01" @default.
- W3176854311 modified "2023-09-23" @default.
- W3176854311 title "Characterization of the CRISPR‐Cas13b systems in Porphyromonas gingivalis" @default.
- W3176854311 doi "https://doi.org/10.1096/fasebj.2019.33.1_supplement.lb250" @default.
- W3176854311 hasPublicationYear "2019" @default.
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