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• W3176959098 abstract "The diurnal presence of the culturable bacterial indicators of fecal contamination in the water environment has been shown to be highly variable over time due to natural die-off and injury from effects of sunlight and other environmental stressors. Molecular analytes of a quantitative polymerase chain reaction (qPCR) method for measuring fecal contamination degrade considerably slower than the alternative of culturable fecal indicator bacteria. The rapid qPCR method holds the promise of more timely notification decisions with respect to postings or closure being made on the basis of microbial water quality samples collected earlier on the same day. In the case of culture-based methods requiring a 24 h or longer incubation period , decisions must be based on samples collected no sooner than the previous day. To examine the effect of this lag in assay results, temporal stability of a molecular Enterococci target analyte with that of traditional culture-based cells is compared using data from USEPA studies conducted between 2003 and 2007 on seven freshwater and marine beaches that were impacted by publicly-owned treatment works. Generally, levels of the molecular indicator were more consistent throughout the day between 8:00 am and 3:00 pm. The difference in temporal consistency is even more pronounced when the 24-h lag in culture-based results is taken into account. • Enterococci indicator bacteria data from EPA NEEAR beach studies are analyzed. • Samples were taken at seven freshwater and marine beaches 3 timesday. • Stability of qPCR vs. cultural (CFU) methods are compared within- and between-day. • CFUs were more likely to exhibit a within-day trend than qPCR. • 27-h vs. 3-h test resulted in higher deviation from real-time levels for CFUs." @default.
• W3176959098 created "2021-07-05" @default.
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• W3176959098 date "2021-09-01" @default.
• W3176959098 modified "2023-10-17" @default.
• W3176959098 title "Comparative stability of assay results of enterococci measured by culture and qPCR over time in bathing beach waters" @default.
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• W3176959098 doi "https://doi.org/10.1016/j.mimet.2021.106274" @default.
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