Matches in SemOpenAlex for { <https://semopenalex.org/work/W3176989197> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W3176989197 abstract "Thyroid hormone (T3) can stimulate protein synthesis and cell growth. NOX1 is a mitogenic oxidase. The aim of this study was to test a novel hypothesis that T3 induces artery smooth muscle cell proliferation by up-regulating NOX1. Immunofluoresence confocal microscopy was used to visualize the sub-cellular localization of NOX1 and TRalpha1 in rat aorta smooth muscle (RASM) cells. Optical sectioning showed that TRalpha1 and NOX1 co-localized around the nucleus. T3 promoted RASM cell proliferation as determined by the fact that T3 significantly increased the number of cytokinesis cells, proliferating cellular nuclear antigen (PCNA) and smooth muscle alpha-actin (SM alpha-actin). T3 increased NOX1 expression at both the transcription (mRNA) and translation (protein) levels as evaluated by RT-PCR and Western blot, respectively. T3 also significantly increased the intracellular ROS production based on the oxidation of 2',7'-dichlorodihydrofluoresein (H2DCF) to a fluorescent 2',7'-dichlorofluoresein (DCF). RNAi silence of TRalpha1 or NOX1 abolished T3-induced intracellular ROS generation and PCNA and SM alpha-actin expression, indicating that TRalpha1 and NOX1 mediated T3-induced RASM cell proliferation. Notably, RNAi silence of TRalpha1 blocked the T3-induced increase in NOX1 expression, whereas silence of NOX1 did not affect TRalpha1 expression, disclosing a new pathway, i.e. T3-TRalpha1-NOX1-cell proliferation. TRalpha1 and NOX1 co-localized around the nucleus. T3 induced RASM cell proliferation by up-regulating NOX1 in a TRalpha1-dependent manner." @default.
- W3176989197 created "2021-07-05" @default.
- W3176989197 creator A5029679885 @default.
- W3176989197 creator A5039591696 @default.
- W3176989197 date "2008-03-01" @default.
- W3176989197 modified "2023-09-22" @default.
- W3176989197 title "Thyroid hormone induces artery smooth muscle cell proliferation: discovery of a new TRα1‐Nox1 pathway" @default.
- W3176989197 doi "https://doi.org/10.1096/fasebj.22.1_supplement.1147.11" @default.
- W3176989197 hasPublicationYear "2008" @default.
- W3176989197 type Work @default.
- W3176989197 sameAs 3176989197 @default.
- W3176989197 citedByCount "1" @default.
- W3176989197 crossrefType "journal-article" @default.
- W3176989197 hasAuthorship W3176989197A5029679885 @default.
- W3176989197 hasAuthorship W3176989197A5039591696 @default.
- W3176989197 hasBestOaLocation W31769891972 @default.
- W3176989197 hasConcept C125705527 @default.
- W3176989197 hasConcept C153911025 @default.
- W3176989197 hasConcept C185592680 @default.
- W3176989197 hasConcept C2776061955 @default.
- W3176989197 hasConcept C2779719074 @default.
- W3176989197 hasConcept C48349386 @default.
- W3176989197 hasConcept C55493867 @default.
- W3176989197 hasConcept C62112901 @default.
- W3176989197 hasConcept C86803240 @default.
- W3176989197 hasConcept C95444343 @default.
- W3176989197 hasConceptScore W3176989197C125705527 @default.
- W3176989197 hasConceptScore W3176989197C153911025 @default.
- W3176989197 hasConceptScore W3176989197C185592680 @default.
- W3176989197 hasConceptScore W3176989197C2776061955 @default.
- W3176989197 hasConceptScore W3176989197C2779719074 @default.
- W3176989197 hasConceptScore W3176989197C48349386 @default.
- W3176989197 hasConceptScore W3176989197C55493867 @default.
- W3176989197 hasConceptScore W3176989197C62112901 @default.
- W3176989197 hasConceptScore W3176989197C86803240 @default.
- W3176989197 hasConceptScore W3176989197C95444343 @default.
- W3176989197 hasIssue "S1" @default.
- W3176989197 hasLocation W31769891971 @default.
- W3176989197 hasLocation W31769891972 @default.
- W3176989197 hasOpenAccess W3176989197 @default.
- W3176989197 hasPrimaryLocation W31769891971 @default.
- W3176989197 hasRelatedWork W1886697770 @default.
- W3176989197 hasRelatedWork W1963506986 @default.
- W3176989197 hasRelatedWork W1982328866 @default.
- W3176989197 hasRelatedWork W2054676466 @default.
- W3176989197 hasRelatedWork W2057651911 @default.
- W3176989197 hasRelatedWork W2063821410 @default.
- W3176989197 hasRelatedWork W2064459944 @default.
- W3176989197 hasRelatedWork W2070330706 @default.
- W3176989197 hasRelatedWork W2074092544 @default.
- W3176989197 hasRelatedWork W2080605195 @default.
- W3176989197 hasRelatedWork W2085679263 @default.
- W3176989197 hasRelatedWork W2087888480 @default.
- W3176989197 hasRelatedWork W2101713491 @default.
- W3176989197 hasRelatedWork W2103243895 @default.
- W3176989197 hasRelatedWork W2114208319 @default.
- W3176989197 hasRelatedWork W2129236688 @default.
- W3176989197 hasRelatedWork W2768498299 @default.
- W3176989197 hasRelatedWork W2885236818 @default.
- W3176989197 hasRelatedWork W2899079934 @default.
- W3176989197 hasRelatedWork W3100858232 @default.
- W3176989197 hasVolume "22" @default.
- W3176989197 isParatext "false" @default.
- W3176989197 isRetracted "false" @default.
- W3176989197 magId "3176989197" @default.
- W3176989197 workType "article" @default.