FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3177065360> ?p ?o ?g. }

• W3177065360 endingPage "170" @default.
• W3177065360 startingPage "123" @default.
• W3177065360 abstract "This chapter discusses the state of the art of synthetic biology in cyanobacteria as well as application areas for synthetic biology within cyanobacteria biotechnology. Our ability to insert or change genes in cyanobacteria, and to control expression of heterologous genes, has advanced significantly over the past decade. Control of transcription by external signal molecules is robust and reliable for model strains, in particular, Synechocystis PCC 6803 where even multiple, orthogonal signals can be used. Of particular importance is an ability to tightly repress gene expression in the absence of an inducer, as heterologous enzymes often impose a growth burden on cells when expressed. Control of gene expression via internal or environmental signals, such as light or other nutrients, is less refined. Control of mRNA translation, for example, with riboswitches or trans-acting RNAs, is particularly useful when combined with transcriptional repression but is sparsely used. Gene editing, using CRISPR/Cas or other nucleases, has been demonstrated in several strains but requires standardization to become widespread. Integration of genes into the genome is common, but expression from the genome suffers can be weak, location dependent, and growth-phase dependent. Expression of genes from episomal plasmids is strong, and there are recent efforts at standardization of these, but plasmids require selection pressure such as antibiotics to maintain for long (>3 weeks) cultivations. Two goals of synthetic biology in cyanobacteria in the near future are to diversify signal inputs via characterization of new transduction proteins and to link multiple signal inputs into a circuit, so that fast, reliable, condition-dependent gene expression can be achieved." @default.
• W3177065360 created "2021-07-05" @default.
• W3177065360 creator A5027625454 @default.
• W3177065360 date "2021-04-30" @default.
• W3177065360 modified "2023-10-01" @default.
• W3177065360 title "Synthetic Biology in Cyanobacteria and Applications for Biotechnology" @default.
• W3177065360 cites W1547106545 @default.
• W3177065360 cites W1579020777 @default.
• W3177065360 cites W1882036684 @default.
• W3177065360 cites W1943504827 @default.
• W3177065360 cites W1957496223 @default.
• W3177065360 cites W1966587420 @default.
• W3177065360 cites W1966645118 @default.
• W3177065360 cites W1969337900 @default.
• W3177065360 cites W1971779492 @default.
• W3177065360 cites W1973256834 @default.
• W3177065360 cites W1975993749 @default.
• W3177065360 cites W1982120559 @default.
• W3177065360 cites W1985773352 @default.
• W3177065360 cites W1987511012 @default.
• W3177065360 cites W1987808620 @default.
• W3177065360 cites W1988517965 @default.
• W3177065360 cites W1994028898 @default.
• W3177065360 cites W1995643560 @default.
• W3177065360 cites W2001426985 @default.
• W3177065360 cites W2002669907 @default.
• W3177065360 cites W2010047991 @default.
• W3177065360 cites W2010392881 @default.
• W3177065360 cites W2015868412 @default.
• W3177065360 cites W2020044960 @default.
• W3177065360 cites W2020652110 @default.
• W3177065360 cites W2027180656 @default.
• W3177065360 cites W2030441409 @default.
• W3177065360 cites W2034718856 @default.
• W3177065360 cites W2044085601 @default.
• W3177065360 cites W2052593484 @default.
• W3177065360 cites W2053512440 @default.
• W3177065360 cites W2061425021 @default.
• W3177065360 cites W2062725155 @default.
• W3177065360 cites W2064590397 @default.
• W3177065360 cites W2065002946 @default.
• W3177065360 cites W2067252347 @default.
• W3177065360 cites W2068219766 @default.
• W3177065360 cites W2073265884 @default.
• W3177065360 cites W2075027571 @default.
• W3177065360 cites W2082740492 @default.
• W3177065360 cites W2085679883 @default.
• W3177065360 cites W2092045125 @default.
• W3177065360 cites W2096233452 @default.
• W3177065360 cites W2100211560 @default.
• W3177065360 cites W2106177878 @default.
• W3177065360 cites W2109110326 @default.
• W3177065360 cites W2111535806 @default.
• W3177065360 cites W2111869546 @default.
• W3177065360 cites W2122280468 @default.
• W3177065360 cites W2123467717 @default.
• W3177065360 cites W2128556638 @default.
• W3177065360 cites W2129558617 @default.
• W3177065360 cites W2130063089 @default.
• W3177065360 cites W2130170434 @default.
• W3177065360 cites W2132124871 @default.
• W3177065360 cites W2133248262 @default.
• W3177065360 cites W2133774018 @default.
• W3177065360 cites W2137543240 @default.
• W3177065360 cites W2140347764 @default.
• W3177065360 cites W2141327755 @default.
• W3177065360 cites W2150195094 @default.
• W3177065360 cites W2154241726 @default.
• W3177065360 cites W2157589351 @default.
• W3177065360 cites W2157737106 @default.
• W3177065360 cites W2160051211 @default.
• W3177065360 cites W2160243998 @default.
• W3177065360 cites W2161011079 @default.
• W3177065360 cites W2161806899 @default.
• W3177065360 cites W2165952036 @default.
• W3177065360 cites W2169502809 @default.
• W3177065360 cites W2169937805 @default.
• W3177065360 cites W2169940438 @default.
• W3177065360 cites W2203862394 @default.
• W3177065360 cites W2234629144 @default.
• W3177065360 cites W2248805887 @default.
• W3177065360 cites W2252701092 @default.
• W3177065360 cites W2259660195 @default.
• W3177065360 cites W2260617737 @default.
• W3177065360 cites W2262101136 @default.
• W3177065360 cites W2315816282 @default.
• W3177065360 cites W2324591890 @default.
• W3177065360 cites W2407174002 @default.
• W3177065360 cites W2470889463 @default.
• W3177065360 cites W2497615362 @default.
• W3177065360 cites W2505933229 @default.
• W3177065360 cites W2506034145 @default.
• W3177065360 cites W2527387613 @default.
• W3177065360 cites W2539146142 @default.
• W3177065360 cites W2539204259 @default.
• W3177065360 cites W2544125587 @default.
• W3177065360 cites W2549394905 @default.
• W3177065360 cites W2549981312 @default.

• next