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- W3177494935 abstract "The sodium coupled amino acid transporter SNAT2 is expressed in mammary gland (MG). There is a special amino acid demand for cellular differentiation during pregnancy and for protein milk secretion during lactation in the MG. The purpose of the present work was to assess the amount and localization of SNAT along pregnancy and lactation in rat MG. Western blot analysis in MG of virgin rats showed the presence of a single band with a MW of 60 kD corresponding to SNAT2 protein. The intensity of this band was maintained during pregnancy and decreased dramatically one day before delivery. During lactation, SNAT2 increased rapidly after 5 days and remains constant along lactation and decreased after post-weaning. Immunohistochemical analysis revealed that MG SNAT2 of virgin rats was exclusively located in adipose cells. During gestation, there was an increase in the staining of SNAT2 in epithelial cells that will synthesize milk and decrease before delivery. During lactation there was a significant increase in the amount of SNAT2 in epithelial cells. Electron immunomicroscopy analysis of SNAT2 in MG during peak of lactation indicated that the SNAT2 protein was retained in vesicles originated from the endoplasmic reticulum and then translocated to the basolateral membrane of the epithelial cells. Our results suggested that presence of SNAT2 in the different cells and stages depends on the needs of the MG. CONACYT grant No 46134" @default.
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- W3177494935 date "2011-04-01" @default.
- W3177494935 modified "2023-10-18" @default.
- W3177494935 title "Cellular localization and protein concentration of the SNAT2 transporter in rat mammary gland during pregnancy and lactation" @default.
- W3177494935 doi "https://doi.org/10.1096/fasebj.25.1_supplement.104.5" @default.
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