Matches in SemOpenAlex for { <https://semopenalex.org/work/W3179229937> ?p ?o ?g. }
- W3179229937 endingPage "1409" @default.
- W3179229937 startingPage "1409" @default.
- W3179229937 abstract "Korean ginseng is one of the most valuable medicinal plants worldwide. However, our understanding of ginseng proteomics is largely limited due to difficulties in the extraction and resolution of ginseng proteins because of the presence of natural contaminants such as polysaccharides, phenols, and glycosides. Here, we compared four different protein extraction methods, namely, TCA/acetone, TCA/acetone–MeOH/chloroform, phenol–TCA/acetone, and phenol–MeOH/chloroform methods. The TCA/acetone–MeOH/chloroform method displayed the highest extraction efficiency, and thus it was used for the comparative proteome profiling of leaf, root, shoot, and fruit by a label-free quantitative proteomics approach. This approach led to the identification of 2604 significantly modulated proteins among four tissues. We could pinpoint differential pathways and proteins associated with ginsenoside biosynthesis, including the methylerythritol 4–phosphate (MEP) pathway, the mevalonate (MVA) pathway, UDP-glycosyltransferases (UGTs), and oxidoreductases (CYP450s). The current study reports an efficient and reproducible method for the isolation of proteins from a wide range of ginseng tissues and provides a detailed organ-based proteome map and a more comprehensive view of enzymatic alterations in ginsenoside biosynthesis." @default.
- W3179229937 created "2021-07-19" @default.
- W3179229937 creator A5010497087 @default.
- W3179229937 creator A5019130342 @default.
- W3179229937 creator A5020995370 @default.
- W3179229937 creator A5022672323 @default.
- W3179229937 creator A5023933191 @default.
- W3179229937 creator A5026587264 @default.
- W3179229937 creator A5028418459 @default.
- W3179229937 creator A5051728611 @default.
- W3179229937 creator A5055850607 @default.
- W3179229937 creator A5057773294 @default.
- W3179229937 creator A5059657982 @default.
- W3179229937 creator A5060609681 @default.
- W3179229937 creator A5071028215 @default.
- W3179229937 creator A5075381376 @default.
- W3179229937 creator A5090461290 @default.
- W3179229937 date "2021-07-09" @default.
- W3179229937 modified "2023-10-06" @default.
- W3179229937 title "Optimization of Protein Isolation and Label-Free Quantitative Proteomic Analysis in Four Different Tissues of Korean Ginseng" @default.
- W3179229937 cites W1742111490 @default.
- W3179229937 cites W1966526845 @default.
- W3179229937 cites W1968601944 @default.
- W3179229937 cites W1981534201 @default.
- W3179229937 cites W1982693050 @default.
- W3179229937 cites W2006986579 @default.
- W3179229937 cites W2016224901 @default.
- W3179229937 cites W2032371924 @default.
- W3179229937 cites W2037199116 @default.
- W3179229937 cites W2039665590 @default.
- W3179229937 cites W2041860051 @default.
- W3179229937 cites W2042007306 @default.
- W3179229937 cites W2044066157 @default.
- W3179229937 cites W2063946272 @default.
- W3179229937 cites W2080201083 @default.
- W3179229937 cites W2102194539 @default.
- W3179229937 cites W2129586833 @default.
- W3179229937 cites W2133517699 @default.
- W3179229937 cites W2137172763 @default.
- W3179229937 cites W2146561348 @default.
- W3179229937 cites W2169892024 @default.
- W3179229937 cites W2195436149 @default.
- W3179229937 cites W2240912536 @default.
- W3179229937 cites W2302658801 @default.
- W3179229937 cites W2408036408 @default.
- W3179229937 cites W2463195069 @default.
- W3179229937 cites W2544360569 @default.
- W3179229937 cites W2592336111 @default.
- W3179229937 cites W2606225602 @default.
- W3179229937 cites W2611604995 @default.
- W3179229937 cites W2692577361 @default.
- W3179229937 cites W2755695275 @default.
- W3179229937 cites W2756780707 @default.
- W3179229937 cites W2793925625 @default.
- W3179229937 cites W2804671419 @default.
- W3179229937 cites W2808317478 @default.
- W3179229937 cites W2885193379 @default.
- W3179229937 cites W2887614713 @default.
- W3179229937 cites W2895433563 @default.
- W3179229937 cites W2899760200 @default.
- W3179229937 cites W2914034392 @default.
- W3179229937 cites W2914131529 @default.
- W3179229937 cites W2947882182 @default.
- W3179229937 cites W2950764390 @default.
- W3179229937 cites W2951137453 @default.
- W3179229937 cites W2971481293 @default.
- W3179229937 cites W3015885315 @default.
- W3179229937 cites W3134978995 @default.
- W3179229937 doi "https://doi.org/10.3390/plants10071409" @default.
- W3179229937 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8309323" @default.
- W3179229937 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34371612" @default.
- W3179229937 hasPublicationYear "2021" @default.
- W3179229937 type Work @default.
- W3179229937 sameAs 3179229937 @default.
- W3179229937 citedByCount "6" @default.
- W3179229937 countsByYear W31792299372022 @default.
- W3179229937 countsByYear W31792299372023 @default.
- W3179229937 crossrefType "journal-article" @default.
- W3179229937 hasAuthorship W3179229937A5010497087 @default.
- W3179229937 hasAuthorship W3179229937A5019130342 @default.
- W3179229937 hasAuthorship W3179229937A5020995370 @default.
- W3179229937 hasAuthorship W3179229937A5022672323 @default.
- W3179229937 hasAuthorship W3179229937A5023933191 @default.
- W3179229937 hasAuthorship W3179229937A5026587264 @default.
- W3179229937 hasAuthorship W3179229937A5028418459 @default.
- W3179229937 hasAuthorship W3179229937A5051728611 @default.
- W3179229937 hasAuthorship W3179229937A5055850607 @default.
- W3179229937 hasAuthorship W3179229937A5057773294 @default.
- W3179229937 hasAuthorship W3179229937A5059657982 @default.
- W3179229937 hasAuthorship W3179229937A5060609681 @default.
- W3179229937 hasAuthorship W3179229937A5071028215 @default.
- W3179229937 hasAuthorship W3179229937A5075381376 @default.
- W3179229937 hasAuthorship W3179229937A5090461290 @default.
- W3179229937 hasBestOaLocation W31792299371 @default.
- W3179229937 hasConcept C104317684 @default.
- W3179229937 hasConcept C104397665 @default.
- W3179229937 hasConcept C135650642 @default.
- W3179229937 hasConcept C142724271 @default.