Matches in SemOpenAlex for { <https://semopenalex.org/work/W3179662431> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W3179662431 endingPage "70" @default.
- W3179662431 startingPage "70" @default.
- W3179662431 abstract "The monkey 20ɑ-hydroxysteroid dehydrogenase (20ɑ-HSD) is an enzyme responsible for the catabolism in converting progesterone into biological inactive 20a-hydroxyprogesterone, thus playing a key role during the estrous cycle or pregnancy and allowing ovulation and parturition to occur in most mammalian animals. We have previously shown that 20ɑ-HSD in pre-ovulation and pre-parturition was highly detected in ovarian and placental tissues and localized mainly in the syncytiotrophoblast of the placenta. In this study, we focus on the analysis of molecular characterization of the monkey 20ɑ-HSD promoter region by using reporter assay systems in a Chinese hamster ovary (CHO) K1 cells. A reporter assay, using constructs (—890-Luc, —513-Luc, —306-Luc, —273-Luc and —70-Luc) of various lengths of the 5'-flanking region, revealed that the region (Ap-1) between —281 and —274 bp was essential for transcriptional activity. The absence of Ap-1 site in -273-Luc dramatically decreased to the control levels. Thus, Ap-1 site (—281→—274) (5'-TGTCTCAT-3') plays a crucial role for monkey 20α-HSD gene transcription. Next, we analyzed the monkey 20α-HSD promoter activity with treatment of prolactin, PGF2α and Forskolin in CHO-K1 cells. The 2005bp monkey promoter-pGL3 was transfected to CHO cells and added various dose-dependent treatments after incubation 24 hours. For PGF2α 0.5, 1, 5, 10 and 50 μM added and incubated during 6 hours. For Forskolin 0.1, 1, 10, 50 and 100 μM added. And finally, the luciferase activity was measured after 24 hours. Also, human prolactin (3μg/ml) was used as inhibitor. In result, PGF2a was response to a dose-dependent activation. On the other hand Forskolin was detected dose-dependent inhibition in the 2005bp 20α-HSD promoterpGL3 construct. However, prolactin was not shown any significant different. Taken together, our results indicate that monkey 20α-HSD promoter activity inhibit by forskolin. Also, PGF2a remarkedly stimulates the activity of monkey 20α-HSD promoter. We demonstrated that monkey 20a-HSD promoter regulate by AP-transcription factor, PGF2a, and Foskolin." @default.
- W3179662431 created "2021-07-19" @default.
- W3179662431 creator A5008362503 @default.
- W3179662431 creator A5015407605 @default.
- W3179662431 creator A5017180454 @default.
- W3179662431 creator A5018742534 @default.
- W3179662431 creator A5089513813 @default.
- W3179662431 date "2013-06-01" @default.
- W3179662431 modified "2023-09-23" @default.
- W3179662431 title "Regulation of Monkey 20α-Hydroxysteroid Dehydrogenase (HSD) Promoter by PGF2a and Foskolin" @default.
- W3179662431 hasPublicationYear "2013" @default.
- W3179662431 type Work @default.
- W3179662431 sameAs 3179662431 @default.
- W3179662431 citedByCount "0" @default.
- W3179662431 crossrefType "journal-article" @default.
- W3179662431 hasAuthorship W3179662431A5008362503 @default.
- W3179662431 hasAuthorship W3179662431A5015407605 @default.
- W3179662431 hasAuthorship W3179662431A5017180454 @default.
- W3179662431 hasAuthorship W3179662431A5018742534 @default.
- W3179662431 hasAuthorship W3179662431A5089513813 @default.
- W3179662431 hasConcept C104317684 @default.
- W3179662431 hasConcept C111335760 @default.
- W3179662431 hasConcept C126322002 @default.
- W3179662431 hasConcept C134018914 @default.
- W3179662431 hasConcept C150194340 @default.
- W3179662431 hasConcept C153911025 @default.
- W3179662431 hasConcept C161733203 @default.
- W3179662431 hasConcept C172680121 @default.
- W3179662431 hasConcept C175656101 @default.
- W3179662431 hasConcept C24998067 @default.
- W3179662431 hasConcept C2776953305 @default.
- W3179662431 hasConcept C2778324911 @default.
- W3179662431 hasConcept C2779234561 @default.
- W3179662431 hasConcept C2779276759 @default.
- W3179662431 hasConcept C2779315647 @default.
- W3179662431 hasConcept C53345823 @default.
- W3179662431 hasConcept C54009773 @default.
- W3179662431 hasConcept C54355233 @default.
- W3179662431 hasConcept C55493867 @default.
- W3179662431 hasConcept C71924100 @default.
- W3179662431 hasConcept C81885089 @default.
- W3179662431 hasConcept C86803240 @default.
- W3179662431 hasConceptScore W3179662431C104317684 @default.
- W3179662431 hasConceptScore W3179662431C111335760 @default.
- W3179662431 hasConceptScore W3179662431C126322002 @default.
- W3179662431 hasConceptScore W3179662431C134018914 @default.
- W3179662431 hasConceptScore W3179662431C150194340 @default.
- W3179662431 hasConceptScore W3179662431C153911025 @default.
- W3179662431 hasConceptScore W3179662431C161733203 @default.
- W3179662431 hasConceptScore W3179662431C172680121 @default.
- W3179662431 hasConceptScore W3179662431C175656101 @default.
- W3179662431 hasConceptScore W3179662431C24998067 @default.
- W3179662431 hasConceptScore W3179662431C2776953305 @default.
- W3179662431 hasConceptScore W3179662431C2778324911 @default.
- W3179662431 hasConceptScore W3179662431C2779234561 @default.
- W3179662431 hasConceptScore W3179662431C2779276759 @default.
- W3179662431 hasConceptScore W3179662431C2779315647 @default.
- W3179662431 hasConceptScore W3179662431C53345823 @default.
- W3179662431 hasConceptScore W3179662431C54009773 @default.
- W3179662431 hasConceptScore W3179662431C54355233 @default.
- W3179662431 hasConceptScore W3179662431C55493867 @default.
- W3179662431 hasConceptScore W3179662431C71924100 @default.
- W3179662431 hasConceptScore W3179662431C81885089 @default.
- W3179662431 hasConceptScore W3179662431C86803240 @default.
- W3179662431 hasIssue "2" @default.
- W3179662431 hasLocation W31796624311 @default.
- W3179662431 hasOpenAccess W3179662431 @default.
- W3179662431 hasPrimaryLocation W31796624311 @default.
- W3179662431 hasRelatedWork W1535264614 @default.
- W3179662431 hasRelatedWork W1824426416 @default.
- W3179662431 hasRelatedWork W1961700264 @default.
- W3179662431 hasRelatedWork W1982166896 @default.
- W3179662431 hasRelatedWork W1986272056 @default.
- W3179662431 hasRelatedWork W1999419061 @default.
- W3179662431 hasRelatedWork W2002202652 @default.
- W3179662431 hasRelatedWork W2036992460 @default.
- W3179662431 hasRelatedWork W2037287442 @default.
- W3179662431 hasRelatedWork W2048659710 @default.
- W3179662431 hasRelatedWork W2078971720 @default.
- W3179662431 hasRelatedWork W2086282830 @default.
- W3179662431 hasRelatedWork W2122149276 @default.
- W3179662431 hasRelatedWork W2140432353 @default.
- W3179662431 hasRelatedWork W2152242805 @default.
- W3179662431 hasRelatedWork W2359589086 @default.
- W3179662431 hasRelatedWork W2360218567 @default.
- W3179662431 hasRelatedWork W2394932623 @default.
- W3179662431 hasRelatedWork W2613957249 @default.
- W3179662431 hasRelatedWork W2947222280 @default.
- W3179662431 hasVolume "37" @default.
- W3179662431 isParatext "false" @default.
- W3179662431 isRetracted "false" @default.
- W3179662431 magId "3179662431" @default.
- W3179662431 workType "article" @default.