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- W3179830719 abstract "RNA-interference (RNAi) is a standard technique for functional genomics in adult mosquitoes. However, RNAi in immature, aquatic mosquito stages has been challenging. Several studies have shown successful larval RNAi, usually in combination with a carrier molecule. Except for one study in malaria mosquito, Anopheles gambiae, none of the previous studies has explored RNAi in mosquito pupae. Even in the study that used RNAi in pupae, double stranded RNA (dsRNA) was introduced by microinjection. Here, we describe a successful method by soaking pupae in water containing dsRNA without any carrier or osmotic challenge. The knockdown persisted into adulthood. We expect that this simple procedure will be useful in the functional analysis of genes that highly express in pupae or newly emerged adults." @default.
- W3179830719 created "2021-07-19" @default.
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- W3179830719 date "2021-07-13" @default.
- W3179830719 modified "2023-09-27" @default.
- W3179830719 title "RNAi by Soaking Aedes aegypti Pupae in dsRNA" @default.
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- W3179830719 doi "https://doi.org/10.3390/insects12070634" @default.
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