FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3180589653> ?p ?o ?g. }

• W3180589653 abstract "Abstract Background Oral squamous cell carcinoma (OSCC) has poor survival rates. There is a pressing need to develop more precise risk assessment methods to tailor clinical treatment. Epigenome-wide association studies in OSCC have not produced a viable biomarker. These studies have relied on methylation array platforms, which are limited in their ability to profile the methylome. In this study, we use MethylCap-Seq (MC-Seq), a comprehensive methylation quantification technique, and brush swab samples, to develop a noninvasive, readily translatable approach to profile the methylome in OSCC patients. Methods Three OSCC patients underwent collection of cancer and contralateral normal tissue and brush swab biopsies, totaling 4 samples for each patient. Epigenome-wide DNA methylation quantification was performed using the SureSelectXT Methyl-Seq platform. DNA quality and methylation site resolution were compared between brush swab and tissue samples. Correlation and methylation value difference were determined for brush swabs vs. tissues for each respective patient and site ( i.e. , cancer or normal). Correlations were calculated between cancer and normal tissues and brush swab samples for each patient to determine the robustness of DNA methylation marks using brush swabs in clinical biomarker studies. Results There were no significant differences in DNA yield between tissue and brush swab samples. Mapping efficiency exceeded 90% across all samples, with no differences between tissue and brush swabs. The average number of CpG sites with at least 10x depth of coverage was 2,716,674 for brush swabs and 2,903,261 for tissues. Matched tissue and brush swabs had excellent correlation (r = 0.913 for cancer samples and r = 0.951 for normal samples). The methylation profile of the top 1,000 CpGs was significantly different between cancer and normal samples (mean p-value = 0.00021) but not different between tissues and brush swabs (mean p-value = 0.11). Conclusions Our results demonstrate that MC-Seq is an efficient platform for epigenome profiling in cancer biomarker studies, with broader methylome coverage than array-based platforms. Brush swab biopsy provides adequate DNA yield for MC-Seq, and taken together, our findings set the stage for development of a non-invasive methylome quantification technique for oral cancer with high translational potential." @default.
• W3180589653 created "2021-07-19" @default.
• W3180589653 creator A5000178954 @default.
• W3180589653 creator A5008145165 @default.
• W3180589653 creator A5011972670 @default.
• W3180589653 creator A5017674134 @default.
• W3180589653 creator A5017918502 @default.
• W3180589653 creator A5021288344 @default.
• W3180589653 creator A5021429069 @default.
• W3180589653 creator A5030395315 @default.
• W3180589653 creator A5036741538 @default.
• W3180589653 creator A5039308697 @default.
• W3180589653 creator A5046173951 @default.
• W3180589653 creator A5055492168 @default.
• W3180589653 creator A5065071537 @default.
• W3180589653 creator A5072286188 @default.
• W3180589653 creator A5072560123 @default.
• W3180589653 creator A5080341418 @default.
• W3180589653 creator A5082834975 @default.
• W3180589653 creator A5086420028 @default.
• W3180589653 creator A5086627090 @default.
• W3180589653 creator A5090838237 @default.
• W3180589653 creator A5091552812 @default.
• W3180589653 date "2021-07-06" @default.
• W3180589653 modified "2023-10-16" @default.
• W3180589653 title "Brush Swab As A Noninvasive Surrogate for Tissue Biopsies in Oral Cancer Patients To Develop Clinically Translatable Epigenetic Biomarkers" @default.
• W3180589653 doi "https://doi.org/10.21203/rs.3.rs-653431/v1" @default.
• W3180589653 hasPublicationYear "2021" @default.
• W3180589653 type Work @default.
• W3180589653 sameAs 3180589653 @default.
• W3180589653 citedByCount "0" @default.
• W3180589653 crossrefType "posted-content" @default.
• W3180589653 hasAuthorship W3180589653A5000178954 @default.
• W3180589653 hasAuthorship W3180589653A5008145165 @default.
• W3180589653 hasAuthorship W3180589653A5011972670 @default.
• W3180589653 hasAuthorship W3180589653A5017674134 @default.
• W3180589653 hasAuthorship W3180589653A5017918502 @default.
• W3180589653 hasAuthorship W3180589653A5021288344 @default.
• W3180589653 hasAuthorship W3180589653A5021429069 @default.
• W3180589653 hasAuthorship W3180589653A5030395315 @default.
• W3180589653 hasAuthorship W3180589653A5036741538 @default.
• W3180589653 hasAuthorship W3180589653A5039308697 @default.
• W3180589653 hasAuthorship W3180589653A5046173951 @default.
• W3180589653 hasAuthorship W3180589653A5055492168 @default.
• W3180589653 hasAuthorship W3180589653A5065071537 @default.
• W3180589653 hasAuthorship W3180589653A5072286188 @default.
• W3180589653 hasAuthorship W3180589653A5072560123 @default.
• W3180589653 hasAuthorship W3180589653A5080341418 @default.
• W3180589653 hasAuthorship W3180589653A5082834975 @default.
• W3180589653 hasAuthorship W3180589653A5086420028 @default.
• W3180589653 hasAuthorship W3180589653A5086627090 @default.
• W3180589653 hasAuthorship W3180589653A5090838237 @default.
• W3180589653 hasAuthorship W3180589653A5091552812 @default.
• W3180589653 hasBestOaLocation W31805896531 @default.
• W3180589653 hasConcept C104317684 @default.
• W3180589653 hasConcept C117717151 @default.
• W3180589653 hasConcept C119599485 @default.
• W3180589653 hasConcept C121608353 @default.
• W3180589653 hasConcept C126322002 @default.
• W3180589653 hasConcept C127413603 @default.
• W3180589653 hasConcept C140173407 @default.
• W3180589653 hasConcept C142724271 @default.
• W3180589653 hasConcept C150194340 @default.
• W3180589653 hasConcept C16108499 @default.
• W3180589653 hasConcept C190727270 @default.
• W3180589653 hasConcept C2775934546 @default.
• W3180589653 hasConcept C2781197716 @default.
• W3180589653 hasConcept C33288867 @default.
• W3180589653 hasConcept C41091548 @default.
• W3180589653 hasConcept C54355233 @default.
• W3180589653 hasConcept C552990157 @default.
• W3180589653 hasConcept C71924100 @default.
• W3180589653 hasConcept C86803240 @default.
• W3180589653 hasConceptScore W3180589653C104317684 @default.
• W3180589653 hasConceptScore W3180589653C117717151 @default.
• W3180589653 hasConceptScore W3180589653C119599485 @default.
• W3180589653 hasConceptScore W3180589653C121608353 @default.
• W3180589653 hasConceptScore W3180589653C126322002 @default.
• W3180589653 hasConceptScore W3180589653C127413603 @default.
• W3180589653 hasConceptScore W3180589653C140173407 @default.
• W3180589653 hasConceptScore W3180589653C142724271 @default.
• W3180589653 hasConceptScore W3180589653C150194340 @default.
• W3180589653 hasConceptScore W3180589653C16108499 @default.
• W3180589653 hasConceptScore W3180589653C190727270 @default.
• W3180589653 hasConceptScore W3180589653C2775934546 @default.
• W3180589653 hasConceptScore W3180589653C2781197716 @default.
• W3180589653 hasConceptScore W3180589653C33288867 @default.
• W3180589653 hasConceptScore W3180589653C41091548 @default.
• W3180589653 hasConceptScore W3180589653C54355233 @default.
• W3180589653 hasConceptScore W3180589653C552990157 @default.
• W3180589653 hasConceptScore W3180589653C71924100 @default.
• W3180589653 hasConceptScore W3180589653C86803240 @default.
• W3180589653 hasLocation W31805896531 @default.
• W3180589653 hasOpenAccess W3180589653 @default.
• W3180589653 hasPrimaryLocation W31805896531 @default.
• W3180589653 hasRelatedWork W1616294352 @default.
• W3180589653 hasRelatedWork W2142309127 @default.
• W3180589653 hasRelatedWork W2906103633 @default.
• W3180589653 hasRelatedWork W2906330508 @default.
• W3180589653 hasRelatedWork W2921058608 @default.

• next