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- W3181125102 abstract "Purified tubulin or microtubules are widely used for in vitro assays related to their function, such as activities of their modifying enzymes and motor protein activity. This paper gave a review for purification tubulin and microtubules (MTs) using two different methods. One method of purification MTs depended on polymerization and depolymerization. MTs were polymerized at 37 °C with taxol in the presence. Another method is to purify tubulin by affinity column chromatography. Stu2p, a kind of MAPs, is able to bind with C-terminal of tubulin. Recombinant Stu2p was purified from expressing bacteria. Then Stu2p was coupled with NHS-activated resins, which were used to purify tubulin from mouse brain. Tubulins purified in this way can also be polymerized at 37 °C." @default.
- W3181125102 created "2021-07-19" @default.
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- W3181125102 date "2021-01-01" @default.
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- W3181125102 title "Purification of tubulin and microtubules from mouse brain" @default.
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- W3181125102 doi "https://doi.org/10.1051/e3sconf/202129001026" @default.
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