Matches in SemOpenAlex for { <https://semopenalex.org/work/W3181199959> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W3181199959 abstract "OBJECTIVE: Develop a model of endosalpingiosis and endometriosis to investigate the mechanisms which facilitate implantation of exfoliated cells in the peritoneum.BACKGROUND: Endosalpingiosis (ES) and endometriosis (EM) are the growth of tubal and endometrial epithelium respectively, outside of their site of origin and have been associated with ovarian cancer. Why this growth happens has not been elucidated since Sampson first proposed the concept of retrograde menstruation nearly 100 years ago. We hypothesized there is retrograde flow of uterine implantation factors which drive ectopic growth. To test this hypothesis, we developed a transgenic fluorescent murine model of ES and EM that successfully demonstrated peritoneal implantation in mice. Using the model, we explored possible influences on implantation.METHODS: tdTomato (tdT) transgenic fluorescent mice were used as donors to allow for visualization of tissues in recipient mice. A progesterone knockout (PKO) of the tdT mouse that blocks implantation factors was created by giving 0.1 mg/kg progesterone on postnatal days 2-10. Metestrus endometrium was induced hormonally in donor tdT and wild-type (WT) female C57BL/6J mice. The tdT oviductal cells and endometrium were then harvested for intraperitoneal injection into synchronized recipient WT mice, receiving minced endometrium alone, minced oviduct alone, or a combination of minced oviduct and WT endometrial tissue (for implantation factors) from non-treated or PKO tdT donors. Ectopic lesions were identified using fluorescence in-vivo imaging and UV light.RESULTS: PKO tdT oviduct had significantly reduced implantation (4 lesions/6 mice, p<0.02) compared to tdT oviduct without treatment (28 lesions/8 mice). Additionally, PKO tdT oviduct implantation (10 lesions/3 mice) may be restored when implanted with WT endometrium (p=0.07). Chi square was used to evaluate for statistical significance.ConclusionImplantation factors required for blastocyst implantation are crucial to initiate ectopic tissue growth. We have developed a novel mouse model of ectopic growth of gynecologic tissues which demonstrates that the removal of implantation factors from endometrium and oviduct reduces ectopic lesions. This model allows us to study the initial step of implantation of these lesions that could give rise to malignancies which will ultimately allow for the development of new prevention and treatment modalities.Citation Format: Jan Sunde, Derek O'Neil, Tiffany Katz, Morgan Wasickanin. Implantation factors assist in the growth of gynecologic tissues in ectopic locations [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr LB248." @default.
- W3181199959 created "2021-07-19" @default.
- W3181199959 creator A5012100141 @default.
- W3181199959 creator A5045544899 @default.
- W3181199959 creator A5046190110 @default.
- W3181199959 creator A5078382069 @default.
- W3181199959 date "2021-07-01" @default.
- W3181199959 modified "2023-10-05" @default.
- W3181199959 title "Abstract LB248: Implantation factors assist in the growth of gynecologic tissues in ectopic locations" @default.
- W3181199959 doi "https://doi.org/10.1158/1538-7445.am2021-lb248" @default.
- W3181199959 hasPublicationYear "2021" @default.
- W3181199959 type Work @default.
- W3181199959 sameAs 3181199959 @default.
- W3181199959 citedByCount "0" @default.
- W3181199959 crossrefType "proceedings-article" @default.
- W3181199959 hasAuthorship W3181199959A5012100141 @default.
- W3181199959 hasAuthorship W3181199959A5045544899 @default.
- W3181199959 hasAuthorship W3181199959A5046190110 @default.
- W3181199959 hasAuthorship W3181199959A5078382069 @default.
- W3181199959 hasBestOaLocation W31811999591 @default.
- W3181199959 hasConcept C102230213 @default.
- W3181199959 hasConcept C104317684 @default.
- W3181199959 hasConcept C121608353 @default.
- W3181199959 hasConcept C126322002 @default.
- W3181199959 hasConcept C134018914 @default.
- W3181199959 hasConcept C141035611 @default.
- W3181199959 hasConcept C142724271 @default.
- W3181199959 hasConcept C150903083 @default.
- W3181199959 hasConcept C16685009 @default.
- W3181199959 hasConcept C207001950 @default.
- W3181199959 hasConcept C2776001837 @default.
- W3181199959 hasConcept C2777088508 @default.
- W3181199959 hasConcept C2777129507 @default.
- W3181199959 hasConcept C2779066055 @default.
- W3181199959 hasConcept C2779522080 @default.
- W3181199959 hasConcept C2779742232 @default.
- W3181199959 hasConcept C55493867 @default.
- W3181199959 hasConcept C71924100 @default.
- W3181199959 hasConcept C86803240 @default.
- W3181199959 hasConceptScore W3181199959C102230213 @default.
- W3181199959 hasConceptScore W3181199959C104317684 @default.
- W3181199959 hasConceptScore W3181199959C121608353 @default.
- W3181199959 hasConceptScore W3181199959C126322002 @default.
- W3181199959 hasConceptScore W3181199959C134018914 @default.
- W3181199959 hasConceptScore W3181199959C141035611 @default.
- W3181199959 hasConceptScore W3181199959C142724271 @default.
- W3181199959 hasConceptScore W3181199959C150903083 @default.
- W3181199959 hasConceptScore W3181199959C16685009 @default.
- W3181199959 hasConceptScore W3181199959C207001950 @default.
- W3181199959 hasConceptScore W3181199959C2776001837 @default.
- W3181199959 hasConceptScore W3181199959C2777088508 @default.
- W3181199959 hasConceptScore W3181199959C2777129507 @default.
- W3181199959 hasConceptScore W3181199959C2779066055 @default.
- W3181199959 hasConceptScore W3181199959C2779522080 @default.
- W3181199959 hasConceptScore W3181199959C2779742232 @default.
- W3181199959 hasConceptScore W3181199959C55493867 @default.
- W3181199959 hasConceptScore W3181199959C71924100 @default.
- W3181199959 hasConceptScore W3181199959C86803240 @default.
- W3181199959 hasLocation W31811999591 @default.
- W3181199959 hasOpenAccess W3181199959 @default.
- W3181199959 hasPrimaryLocation W31811999591 @default.
- W3181199959 hasRelatedWork W1997007826 @default.
- W3181199959 hasRelatedWork W2014057229 @default.
- W3181199959 hasRelatedWork W2053362447 @default.
- W3181199959 hasRelatedWork W2066443608 @default.
- W3181199959 hasRelatedWork W2107761980 @default.
- W3181199959 hasRelatedWork W2137013713 @default.
- W3181199959 hasRelatedWork W2149799471 @default.
- W3181199959 hasRelatedWork W2395449121 @default.
- W3181199959 hasRelatedWork W2612774447 @default.
- W3181199959 hasRelatedWork W4249903769 @default.
- W3181199959 isParatext "false" @default.
- W3181199959 isRetracted "false" @default.
- W3181199959 magId "3181199959" @default.
- W3181199959 workType "article" @default.