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• W3181911503 abstract "Although covalent organic frameworks (COFs) have earned significant interest in separation applications, the use of COFs in biomolecule separation remains unexplored. We examined the ionic COF Py-BPy2+–COF as an ion exchange material for biomolecule separation. After characterizing the properties of the synthesized COF with a variety of techniques, binding experiments with both large and small biomolecules were performed. High adsorption capacities of amino acids with different hydrophobicity and charge, as well as proteins of different isoelectric points and molecular weights, were determined in batch equilibrium experiments. Desorption experiments with mixtures of model proteins demonstrated an ability to successfully separate one protein from another with the selectivity hypothesized to be a combination of the isoelectric point, hydrophobicity, and ability to penetrate the crystalline material. Overall, the results demonstrated that Py-BPy2+–COF can be exploited as a robust crystalline anion exchange biomolecule separation material." @default.
• W3181911503 created "2021-07-19" @default.
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• W3181911503 date "2021-07-15" @default.
• W3181911503 modified "2023-10-05" @default.
• W3181911503 title "Cationic Covalent Organic Framework as an Ion Exchange Material for Efficient Adsorptive Separation of Biomolecules" @default.
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• W3181911503 doi "https://doi.org/10.1021/acsami.1c11270" @default.
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