Matches in SemOpenAlex for { <https://semopenalex.org/work/W318234497> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W318234497 endingPage "H336" @default.
- W318234497 startingPage "H328" @default.
- W318234497 abstract "We compared specific [3H]saxitoxin (STX) binding to isolated sheep ventricular sarcolemmal vesicles with inhibition of maximal action potential upstroke velocity (V max) by STX and tetrodotoxin (TTX) in sheep trabeculae carneae. In sarcolemmal vesicles purified 30 to 40 times over cardiac homogenate, STX binding at 0 degrees C in Na-free solution exhibited both high-affinity sites (KD = 0.22 +/- 0.05 nM, n = 85 +/- 13 fmol/mg protein) and low-affinity sites (KD = 11 +/- 4 nM, n = 360 +/- 42). The STX-inhibition constant for V max in Tyrode solution at 37 degrees C was 280 nM. TTX was approximately 10% as effective as STX in displacing bound [3H]STX and inhibiting V max. Allowing for different experimental conditions during [3H]STX binding and V max measurements, we suggest that the low-affinity sites are physiologically relevant fast Na+ channels of myocardial cells. Combining morphometric data for plasmalemmal area of mammalian cardiac myocytes with n for low-affinity sites, we estimate 3.6-7.6 fast Na+ channels/micron2 plasmalemma." @default.
- W318234497 created "2016-06-24" @default.
- W318234497 creator A5008274054 @default.
- W318234497 creator A5012203185 @default.
- W318234497 creator A5038171309 @default.
- W318234497 creator A5077964034 @default.
- W318234497 creator A5091261782 @default.
- W318234497 date "1985-08-01" @default.
- W318234497 modified "2023-09-26" @default.
- W318234497 title "Saxitoxin binding and fast sodium channel inhibition in sheep heart plasma membrane" @default.
- W318234497 doi "https://doi.org/10.1152/ajpheart.1985.249.2.h328" @default.
- W318234497 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2411152" @default.
- W318234497 hasPublicationYear "1985" @default.
- W318234497 type Work @default.
- W318234497 sameAs 318234497 @default.
- W318234497 citedByCount "4" @default.
- W318234497 crossrefType "journal-article" @default.
- W318234497 hasAuthorship W318234497A5008274054 @default.
- W318234497 hasAuthorship W318234497A5012203185 @default.
- W318234497 hasAuthorship W318234497A5038171309 @default.
- W318234497 hasAuthorship W318234497A5077964034 @default.
- W318234497 hasAuthorship W318234497A5091261782 @default.
- W318234497 hasConcept C107824862 @default.
- W318234497 hasConcept C12554922 @default.
- W318234497 hasConcept C130316041 @default.
- W318234497 hasConcept C134018914 @default.
- W318234497 hasConcept C178790620 @default.
- W318234497 hasConcept C185592680 @default.
- W318234497 hasConcept C207200792 @default.
- W318234497 hasConcept C2776741303 @default.
- W318234497 hasConcept C2777367657 @default.
- W318234497 hasConcept C2777646408 @default.
- W318234497 hasConcept C41625074 @default.
- W318234497 hasConcept C50952357 @default.
- W318234497 hasConcept C537181965 @default.
- W318234497 hasConcept C55493867 @default.
- W318234497 hasConcept C86803240 @default.
- W318234497 hasConceptScore W318234497C107824862 @default.
- W318234497 hasConceptScore W318234497C12554922 @default.
- W318234497 hasConceptScore W318234497C130316041 @default.
- W318234497 hasConceptScore W318234497C134018914 @default.
- W318234497 hasConceptScore W318234497C178790620 @default.
- W318234497 hasConceptScore W318234497C185592680 @default.
- W318234497 hasConceptScore W318234497C207200792 @default.
- W318234497 hasConceptScore W318234497C2776741303 @default.
- W318234497 hasConceptScore W318234497C2777367657 @default.
- W318234497 hasConceptScore W318234497C2777646408 @default.
- W318234497 hasConceptScore W318234497C41625074 @default.
- W318234497 hasConceptScore W318234497C50952357 @default.
- W318234497 hasConceptScore W318234497C537181965 @default.
- W318234497 hasConceptScore W318234497C55493867 @default.
- W318234497 hasConceptScore W318234497C86803240 @default.
- W318234497 hasIssue "2" @default.
- W318234497 hasLocation W3182344971 @default.
- W318234497 hasLocation W3182344972 @default.
- W318234497 hasOpenAccess W318234497 @default.
- W318234497 hasPrimaryLocation W3182344971 @default.
- W318234497 hasRelatedWork W1928784962 @default.
- W318234497 hasRelatedWork W1971563086 @default.
- W318234497 hasRelatedWork W1971990477 @default.
- W318234497 hasRelatedWork W1979777090 @default.
- W318234497 hasRelatedWork W2000830398 @default.
- W318234497 hasRelatedWork W2061904925 @default.
- W318234497 hasRelatedWork W2062689691 @default.
- W318234497 hasRelatedWork W2070676751 @default.
- W318234497 hasRelatedWork W2092896974 @default.
- W318234497 hasRelatedWork W2123369785 @default.
- W318234497 hasVolume "249" @default.
- W318234497 isParatext "false" @default.
- W318234497 isRetracted "false" @default.
- W318234497 magId "318234497" @default.
- W318234497 workType "article" @default.