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• W3182352010 abstract "Cavitation can occur when liquids are exposed to pressure waves of sufficient amplitude, producing rapidly expanding and collapsing gas bubbles that generate localized regions of high energy dissipation. When vials containing insulin were subjected to mechanical shock or when ultrasound was applied to the vials, the resulting cavitation events induced formation of insulin amyloid fibril nuclei that were detected by transmission electron microscopy and quantified by fluorescence spectroscopy following staining with the amyloid-sensitive dye thioflavin-T. Dropping insulin solutions in glass vials produced only minute amounts of insulin fibril nuclei, which could be detected by allowing the nuclei to grow. Cavitation-induced formation of amyloid aggregates may be relevant for iatrogenic insulin deposition disease, where insulin fibrils formed in vitro prior to administration to patients could serve as nuclei for growing fibril deposits in vivo." @default.
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• W3182352010 date "2021-07-14" @default.
• W3182352010 modified "2023-10-14" @default.
• W3182352010 title "Insulin Fibril Formation Caused by Mechanical Shock and Cavitation" @default.
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• W3182352010 doi "https://doi.org/10.1021/acs.jpcb.1c01997" @default.
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