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• W3182767444 endingPage "e1009713" @default.
• W3182767444 startingPage "e1009713" @default.
• W3182767444 abstract "Salmonella hijack host machinery in order to invade cells and establish infection. While considerable work has described the role of host proteins in invasion, much less is known regarding how natural variation in these invasion-associated host proteins affects Salmonella pathogenesis. Here we leveraged a candidate cellular GWAS screen to identify natural genetic variation in the ARHGEF26 (Rho Guanine Nucleotide Exchange Factor 26) gene that renders lymphoblastoid cells susceptible to Salmonella Typhi and Typhimurium invasion. Experimental follow-up redefined ARHGEF26's role in Salmonella epithelial cell infection. Specifically, we identified complex serovar-by-host interactions whereby ARHGEF26 stimulation of S. Typhi and S. Typhimurium invasion into host cells varied in magnitude and effector-dependence based on host cell type. While ARHGEF26 regulated SopB- and SopE-mediated S. Typhi (but not S. Typhimurium) infection of HeLa cells, the largest effect of ARHGEF26 was observed with S. Typhimurium in polarized MDCK cells through a SopB- and SopE2-independent mechanism. In both cell types, knockdown of the ARHGEF26-associated protein DLG1 resulted in a similar phenotype and serovar specificity. Importantly, we show that ARHGEF26 plays a critical role in S. Typhimurium pathogenesis by contributing to bacterial burden in the enteric fever murine model, as well as inflammation in the colitis infection model. In the enteric fever model, SopB and SopE2 are required for the effects of Arhgef26 deletion on bacterial burden, and the impact of sopB and sopE2 deletion in turn required ARHGEF26. In contrast, SopB and SopE2 were not required for the impacts of Arhgef26 deletion on colitis. A role for ARHGEF26 on inflammation was also seen in cells, as knockdown reduced IL-8 production in HeLa cells. Together, these data reveal pleiotropic roles for ARHGEF26 during infection and highlight that many of the interactions that occur during infection that are thought to be well understood likely have underappreciated complexity." @default.
• W3182767444 created "2021-07-19" @default.
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• W3182767444 date "2021-07-09" @default.
• W3182767444 modified "2023-10-14" @default.
• W3182767444 title "ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice" @default.
• W3182767444 cites W1522528227 @default.
• W3182767444 cites W1653610611 @default.
• W3182767444 cites W1687906393 @default.
• W3182767444 cites W1965933688 @default.
• W3182767444 cites W1966042774 @default.
• W3182767444 cites W1968260093 @default.
• W3182767444 cites W1970698773 @default.
• W3182767444 cites W1971928903 @default.
• W3182767444 cites W1979201752 @default.
• W3182767444 cites W1980431822 @default.
• W3182767444 cites W1981659779 @default.
• W3182767444 cites W1985287781 @default.
• W3182767444 cites W1987691266 @default.
• W3182767444 cites W1989630349 @default.
• W3182767444 cites W1990451002 @default.
• W3182767444 cites W1991719368 @default.
• W3182767444 cites W1996832131 @default.
• W3182767444 cites W1997119394 @default.
• W3182767444 cites W1999768397 @default.
• W3182767444 cites W2002547691 @default.
• W3182767444 cites W2005194404 @default.
• W3182767444 cites W2018838463 @default.
• W3182767444 cites W2020157212 @default.
• W3182767444 cites W2022556760 @default.
• W3182767444 cites W2025763309 @default.
• W3182767444 cites W2025943989 @default.
• W3182767444 cites W2055186636 @default.
• W3182767444 cites W2062359771 @default.
• W3182767444 cites W2063742383 @default.
• W3182767444 cites W2066693766 @default.
• W3182767444 cites W2068715852 @default.
• W3182767444 cites W2070334399 @default.
• W3182767444 cites W2076997718 @default.
• W3182767444 cites W2077418900 @default.
• W3182767444 cites W2077879873 @default.
• W3182767444 cites W2081226705 @default.
• W3182767444 cites W2084120324 @default.
• W3182767444 cites W2085371447 @default.
• W3182767444 cites W2090135226 @default.
• W3182767444 cites W2096322420 @default.
• W3182767444 cites W2099085143 @default.
• W3182767444 cites W2109890918 @default.
• W3182767444 cites W2115235932 @default.
• W3182767444 cites W2116137883 @default.
• W3182767444 cites W2120723299 @default.
• W3182767444 cites W2123484637 @default.
• W3182767444 cites W2123675453 @default.
• W3182767444 cites W2124519481 @default.
• W3182767444 cites W2124805544 @default.
• W3182767444 cites W2128091547 @default.
• W3182767444 cites W2137665214 @default.
• W3182767444 cites W2146098592 @default.
• W3182767444 cites W2150254929 @default.
• W3182767444 cites W2150618641 @default.
• W3182767444 cites W2157295579 @default.
• W3182767444 cites W2159577904 @default.
• W3182767444 cites W2163013660 @default.
• W3182767444 cites W2166320441 @default.
• W3182767444 cites W2167279371 @default.
• W3182767444 cites W2195359644 @default.
• W3182767444 cites W2501600422 @default.
• W3182767444 cites W2611089820 @default.
• W3182767444 cites W2743970427 @default.
• W3182767444 cites W2747041325 @default.
• W3182767444 cites W2759562520 @default.
• W3182767444 cites W2766771070 @default.
• W3182767444 cites W2791669890 @default.
• W3182767444 cites W2792442926 @default.
• W3182767444 cites W2803479625 @default.
• W3182767444 cites W2808891613 @default.
• W3182767444 cites W2883223679 @default.
• W3182767444 cites W2889919580 @default.
• W3182767444 cites W2898900122 @default.
• W3182767444 cites W2952719656 @default.
• W3182767444 cites W2955076607 @default.
• W3182767444 cites W2971165205 @default.
• W3182767444 cites W2998342995 @default.
• W3182767444 cites W3021494264 @default.
• W3182767444 cites W3163618648 @default.
• W3182767444 doi "https://doi.org/10.1371/journal.ppat.1009713" @default.
• W3182767444 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8294491" @default.
• W3182767444 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34242364" @default.
• W3182767444 hasPublicationYear "2021" @default.
• W3182767444 type Work @default.

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