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- W3182916860 abstract "Abstract Quantification of low abundant membrane-binding proteins such as transcriptional factors and chaperones has been proved difficult even with the most sophisticated analytical technologies. Here we exploit and optimise the non-invasive Fluorescence Correlation Spectroscopy (FCS) for quantitation of low abundance proteins and as proof of principle we choose two interacting proteins involved in fission of mitochondria in yeast, Fis1p and Mdv1p. In Saccharomyces cerevisiae the recruitment of Fis1p and Mdv1p to mitochondria is essential for the scission of the organelles and the retention of functional mitochondrial structures in the cell. We used FCS in single, GFP-labelled live yeast cells to quantify the protein abundance in homozygote and heterozygote cells, and to investigate the impact of the environments on protein copy number, bound/unbound protein state and mobility kinetics. Both proteins were observed to localise predominantly at mitochondrial structures with the Mdv1p bound state increasing significantly in a strictly respiratory environment. Moreover, a compensatory mechanism which controls Fis1p abundance upon deletion of one allele was observed in Fis1p but not in Mdv1p, suggesting differential regulation of Fis1p and Mdv1p protein expression." @default.
- W3182916860 created "2021-07-19" @default.
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- W3182916860 date "2021-07-10" @default.
- W3182916860 modified "2023-09-27" @default.
- W3182916860 title "Quantitative characterisation of low abundant yeast mitochondrial proteins reveals compensation for haplo-insufficiency in different environments" @default.
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- W3182916860 doi "https://doi.org/10.1101/2021.07.09.451775" @default.
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