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- W3183105473 abstract "Abstract Background : The advanced sensitive STR kits applied in forensic DNA typing techniques can cause challenging issues when evidence samples are contaminated with minute quantities of DNA from another source such as forensic analysts or crime scene examiners. Methods and Results: In this study, laboratory air and surfaces, gloves, tools, and equipment were evaluated as potential sources of contaminating DNA. Different sterilization methods were tested for their ability to efficiently eliminate DNA in a sample. Inactivation methods included 10% bleach, ethanol, UV light and DNA-ExitusPlus IF. Exposure to the different inactivation protocols for varying periods of time was performed in two lab settings: low template DNA and DNA database labs. Surfaces were swabbed and any adhering DNA was quantified using HID real-time PCR. Results were detected using HID Real-Time PCR Analysis Software v1.2 and GeneMapper ID-X Software v1.4. Conclusions: It was concluded that most of the DNA decontamination methods are not suitable for highly sensitive and precision STR kits such as GlobalFiler PCR Amplification Kit. The most suitable tested method was using DNA-ExitusPlus IF with the incubation time increased from 10 to 15 minutes" @default.
- W3183105473 created "2021-07-19" @default.
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- W3183105473 date "2021-07-12" @default.
- W3183105473 modified "2023-09-23" @default.
- W3183105473 title "Comparison between Various DNA Sterilization Procedures Applied in Forensic Analysis" @default.
- W3183105473 doi "https://doi.org/10.21203/rs.3.rs-661190/v1" @default.
- W3183105473 hasPublicationYear "2021" @default.
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