Matches in SemOpenAlex for { <https://semopenalex.org/work/W3183201069> ?p ?o ?g. }
- W3183201069 abstract "Abstract Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in their compositions and structures. However, it is not clear how these events are coordinated and how they integrate with regulation of molecular crowding. We use the budding yeast Saccharomyces cerevisiae as a model system to study these questions using recent progress in optical fluorescence microscopy and crowding sensing probe technology. We used a Förster Resonance Energy Transfer (FRET) based sensor, illuminated by confocal microscopy for high throughput analyses and Slimfield microscopy for single-molecule resolution, to quantify molecular crowding. We determine crowding in response to cellular growth of both mother and daughter cells, in addition to osmotic stress, and reveal hot spots of crowding across the bud neck in the burgeoning daughter cell. This crowding might be rationalized by the packing of inherited material, like the vacuole, from mother cells. We discuss recent advances in understanding the role of crowding in cellular regulation and key current challenges and conclude by presenting our recent advances in optimizing FRET-based measurements of crowding whilst simultaneously imaging a third color, which can be used as a marker that labels organelle membranes. Our approaches can be combined with synchronised cell populations to increase experimental throughput and correlate molecular crowding information with different stages in the cell cycle." @default.
- W3183201069 created "2021-08-02" @default.
- W3183201069 creator A5006014233 @default.
- W3183201069 creator A5013089539 @default.
- W3183201069 creator A5020568316 @default.
- W3183201069 creator A5060446335 @default.
- W3183201069 creator A5074311677 @default.
- W3183201069 creator A5079996725 @default.
- W3183201069 date "2021-07-30" @default.
- W3183201069 modified "2023-09-27" @default.
- W3183201069 title "Investigating molecular crowding during cell division in budding yeast with FRET" @default.
- W3183201069 cites W139121046 @default.
- W3183201069 cites W1528126252 @default.
- W3183201069 cites W1535564633 @default.
- W3183201069 cites W1539922742 @default.
- W3183201069 cites W1560733935 @default.
- W3183201069 cites W178334492 @default.
- W3183201069 cites W1848934355 @default.
- W3183201069 cites W1963578989 @default.
- W3183201069 cites W1963813628 @default.
- W3183201069 cites W1965012924 @default.
- W3183201069 cites W1966656796 @default.
- W3183201069 cites W1969589500 @default.
- W3183201069 cites W1970924670 @default.
- W3183201069 cites W1975468372 @default.
- W3183201069 cites W1976488701 @default.
- W3183201069 cites W1982481819 @default.
- W3183201069 cites W1983903436 @default.
- W3183201069 cites W1990032691 @default.
- W3183201069 cites W2001804668 @default.
- W3183201069 cites W2004908076 @default.
- W3183201069 cites W2004984389 @default.
- W3183201069 cites W2006192534 @default.
- W3183201069 cites W2009891171 @default.
- W3183201069 cites W2011301426 @default.
- W3183201069 cites W2012500478 @default.
- W3183201069 cites W2013673237 @default.
- W3183201069 cites W2015159529 @default.
- W3183201069 cites W2019215631 @default.
- W3183201069 cites W2019377841 @default.
- W3183201069 cites W2020017362 @default.
- W3183201069 cites W2020677693 @default.
- W3183201069 cites W2024677399 @default.
- W3183201069 cites W2028758851 @default.
- W3183201069 cites W2028921845 @default.
- W3183201069 cites W2034728705 @default.
- W3183201069 cites W2035571071 @default.
- W3183201069 cites W2035870580 @default.
- W3183201069 cites W2036769067 @default.
- W3183201069 cites W2037747220 @default.
- W3183201069 cites W2045929752 @default.
- W3183201069 cites W2050400409 @default.
- W3183201069 cites W2050936151 @default.
- W3183201069 cites W2054317237 @default.
- W3183201069 cites W2054543531 @default.
- W3183201069 cites W2057587771 @default.
- W3183201069 cites W2059665827 @default.
- W3183201069 cites W2060512844 @default.
- W3183201069 cites W2060872711 @default.
- W3183201069 cites W2066341288 @default.
- W3183201069 cites W2070955545 @default.
- W3183201069 cites W2072607401 @default.
- W3183201069 cites W2074137927 @default.
- W3183201069 cites W2076153333 @default.
- W3183201069 cites W2076393678 @default.
- W3183201069 cites W2077148282 @default.
- W3183201069 cites W2080629737 @default.
- W3183201069 cites W2083131603 @default.
- W3183201069 cites W2084572582 @default.
- W3183201069 cites W2085216961 @default.
- W3183201069 cites W2088858313 @default.
- W3183201069 cites W2094353613 @default.
- W3183201069 cites W2099636504 @default.
- W3183201069 cites W2101955906 @default.
- W3183201069 cites W2109262751 @default.
- W3183201069 cites W2110180483 @default.
- W3183201069 cites W2112553639 @default.
- W3183201069 cites W2114123456 @default.
- W3183201069 cites W2114587995 @default.
- W3183201069 cites W2118360452 @default.
- W3183201069 cites W2118956583 @default.
- W3183201069 cites W2123256370 @default.
- W3183201069 cites W2124466665 @default.
- W3183201069 cites W2124779290 @default.
- W3183201069 cites W2125598648 @default.
- W3183201069 cites W2126525177 @default.
- W3183201069 cites W2134949824 @default.
- W3183201069 cites W2136626347 @default.
- W3183201069 cites W2143991512 @default.
- W3183201069 cites W2148146668 @default.
- W3183201069 cites W2148175003 @default.
- W3183201069 cites W2149110160 @default.
- W3183201069 cites W2150766477 @default.
- W3183201069 cites W2158308821 @default.
- W3183201069 cites W2159100436 @default.
- W3183201069 cites W2165847215 @default.
- W3183201069 cites W2167858141 @default.
- W3183201069 cites W2169600966 @default.
- W3183201069 cites W2169736187 @default.
- W3183201069 cites W2307399619 @default.