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• W3183252304 abstract "Neisseria gonorrhoeae (Ng) is highly resistant to treatment, and there is an urgent need for new treatments to alleviate gonococcal resistance caused by antibiotic monotherapy. The antimicrobial effect and mechanism of plasma-activated liquid (PAL) on Ng were evaluated in this study. Upon PAL treatment, extensively analyses on cell culturability, metabolic capacity, intracellular reactive oxygen species (ROS),membrane integrity and nucleic acids for Ng were carried out and significant antimicrobial effects observed.PAL exerted antibacterial effect on Ng and induced bacterial death (6.71-log) following immersion for 30 min and treatment for 120 s. However, bacterial viability test revealed that after immersion in the same PAL, 10.17% of bacteria retained their metabolic capacity. This indicates that bacteria enter a physiological viable but non-culturable state to protect themselves from environmental stress. Confocal fluorescence microscopy and transmission electron microscopy demonstrated that PAL exerts bactericidal effect on Ng and disrupts its morphological structure. PAL may upregulate inflammatory factors and genes to modulate the resistance of Ng and affect the immune status of the host during infection." @default.
• W3183252304 created "2021-08-02" @default.
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• W3183252304 date "2021-01-01" @default.
• W3183252304 modified "2023-10-11" @default.
• W3183252304 title "In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic <i>Neisseria gonorrhoeae</i>" @default.
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• W3183252304 doi "https://doi.org/10.1080/21655979.2021.1955548" @default.
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