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- W3183306098 abstract "Abstract The airways of people with cystic fibrosis (CF) often harbour diverse polymicrobial communities. These airway infections can be impossible to resolve though antibiotic intervention, even though isolates of the individual species present are susceptible to the treatment when tested in vitro . This suggests that susceptibility to antimicrobial agents may be altered in the presence of other microbial species. In this work, we investigate how polymicrobial cultures of key CF-associated species respond to challenge with species-specific antimicrobial agents; colistin (targets Pseudomonas aeruginosa ), fusidic acid (targets Staphylococcus aureus ) and fluconazole (targets Candida albicans ). We found that, compared with growth in axenic cultures, the target organism was protected (sometimes by several orders of magnitude) from the effect(s) of the antimicrobial agent when grown in a polymicrobial culture. This decreased antimicrobial efficacy in polymicrobial cultures was found to have both phenotypic and inherited components. Whole genome sequencing of the colistin-resistant P. aeruginosa isolates revealed single nucleotide polymorphisms and indels in genes encoding lipopolysaccharide (LPS) biosynthesis or pilus biogenesis. Colistin resistance associated with loss-of-function mutations in the LPS biosynthetic gene, wzy , could be complemented by expression of the wild-type wzy gene in trans . Our findings indicate that the polymicrobial nature of the CF airways is likely to have a significant impact on the clinical response to antimicrobial therapy." @default.
- W3183306098 created "2021-08-02" @default.
- W3183306098 creator A5029109193 @default.
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- W3183306098 date "2021-07-20" @default.
- W3183306098 modified "2023-10-16" @default.
- W3183306098 title "The efficacy of antimicrobial agents is decreased in a polymicrobial environment" @default.
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- W3183306098 cites W1970581996 @default.
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- W3183306098 cites W1994917518 @default.
- W3183306098 cites W1996064131 @default.
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- W3183306098 cites W2038643099 @default.
- W3183306098 cites W2040539560 @default.
- W3183306098 cites W2054788361 @default.
- W3183306098 cites W2056538792 @default.
- W3183306098 cites W2062817953 @default.
- W3183306098 cites W2066363596 @default.
- W3183306098 cites W2066396905 @default.
- W3183306098 cites W2085630244 @default.
- W3183306098 cites W2086358238 @default.
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- W3183306098 cites W2094167812 @default.
- W3183306098 cites W2095627741 @default.
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- W3183306098 cites W2214796399 @default.
- W3183306098 cites W2267475111 @default.
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- W3183306098 cites W2346117618 @default.
- W3183306098 cites W2401617566 @default.
- W3183306098 cites W2511655118 @default.
- W3183306098 cites W2530495088 @default.
- W3183306098 cites W2567281264 @default.
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- W3183306098 cites W2765466553 @default.
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- W3183306098 cites W2990162666 @default.
- W3183306098 cites W2991029373 @default.
- W3183306098 cites W2992075105 @default.
- W3183306098 cites W3005330807 @default.
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- W3183306098 cites W4214721068 @default.
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- W3183306098 doi "https://doi.org/10.1101/2021.07.20.453069" @default.
- W3183306098 hasPublicationYear "2021" @default.
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