Matches in SemOpenAlex for { <https://semopenalex.org/work/W3183375372> ?p ?o ?g. }
- W3183375372 endingPage "501" @default.
- W3183375372 startingPage "501" @default.
- W3183375372 abstract "The voltage-gated sodium channel Nav1.8 is linked to neuropathic and inflammatory pain, highlighting the potential to serve as a drug target. However, the biophysical mechanisms that regulate Nav1.8 activation and inactivation gating are not completely understood. Progress has been hindered by a lack of biochemical tools for examining Nav1.8 gating mechanisms. Arizona bark scorpion (Centruroides sculpturatus) venom proteins inhibit Nav1.8 and block pain in grasshopper mice (Onychomys torridus). These proteins provide tools for examining Nav1.8 structure–activity relationships. To identify proteins that inhibit Nav1.8 activity, venom samples were fractioned using liquid chromatography (reversed-phase and ion exchange). A recombinant Nav1.8 clone expressed in ND7/23 cells was used to identify subfractions that inhibited Nav1.8 Na+ current. Mass-spectrometry-based bottom-up proteomic analyses identified unique peptides from inhibitory subfractions. A search of the peptides against the AZ bark scorpion venom gland transcriptome revealed four novel proteins between 40 and 60% conserved with venom proteins from scorpions in four genera (Centruroides, Parabuthus, Androctonus, and Tityus). Ranging from 63 to 82 amino acids, each primary structure includes eight cysteines and a “CXCE” motif, where X = an aromatic residue (tryptophan, tyrosine, or phenylalanine). Electrophysiology data demonstrated that the inhibitory effects of bioactive subfractions can be removed by hyperpolarizing the channels, suggesting that proteins may function as gating modifiers as opposed to pore blockers." @default.
- W3183375372 created "2021-08-02" @default.
- W3183375372 creator A5003678935 @default.
- W3183375372 creator A5016388201 @default.
- W3183375372 creator A5017965947 @default.
- W3183375372 creator A5029812885 @default.
- W3183375372 creator A5035195284 @default.
- W3183375372 creator A5056826659 @default.
- W3183375372 creator A5061968742 @default.
- W3183375372 creator A5062788088 @default.
- W3183375372 creator A5077534501 @default.
- W3183375372 creator A5083556957 @default.
- W3183375372 creator A5083736594 @default.
- W3183375372 creator A5089949345 @default.
- W3183375372 date "2021-07-18" @default.
- W3183375372 modified "2023-10-15" @default.
- W3183375372 title "Identification and Characterization of Novel Proteins from Arizona Bark Scorpion Venom That Inhibit Nav1.8, a Voltage-Gated Sodium Channel Regulator of Pain Signaling" @default.
- W3183375372 cites W1836738800 @default.
- W3183375372 cites W1844967107 @default.
- W3183375372 cites W1964253046 @default.
- W3183375372 cites W1965522673 @default.
- W3183375372 cites W1974937986 @default.
- W3183375372 cites W1977073662 @default.
- W3183375372 cites W1978939719 @default.
- W3183375372 cites W1983187084 @default.
- W3183375372 cites W1988556034 @default.
- W3183375372 cites W1991661278 @default.
- W3183375372 cites W1992670328 @default.
- W3183375372 cites W1997333327 @default.
- W3183375372 cites W1999893599 @default.
- W3183375372 cites W2003356426 @default.
- W3183375372 cites W2008012739 @default.
- W3183375372 cites W2017130656 @default.
- W3183375372 cites W2022339065 @default.
- W3183375372 cites W2023739994 @default.
- W3183375372 cites W2026710934 @default.
- W3183375372 cites W2029287987 @default.
- W3183375372 cites W2035070309 @default.
- W3183375372 cites W2042301702 @default.
- W3183375372 cites W2051109362 @default.
- W3183375372 cites W2053307645 @default.
- W3183375372 cites W2061695857 @default.
- W3183375372 cites W2064129686 @default.
- W3183375372 cites W2075715972 @default.
- W3183375372 cites W2077047903 @default.
- W3183375372 cites W2082311140 @default.
- W3183375372 cites W2089073273 @default.
- W3183375372 cites W2093154217 @default.
- W3183375372 cites W2108438677 @default.
- W3183375372 cites W2108974814 @default.
- W3183375372 cites W2122467599 @default.
- W3183375372 cites W2125402042 @default.
- W3183375372 cites W2138809708 @default.
- W3183375372 cites W2141005071 @default.
- W3183375372 cites W2152695330 @default.
- W3183375372 cites W2154594506 @default.
- W3183375372 cites W2162723169 @default.
- W3183375372 cites W2172044021 @default.
- W3183375372 cites W2197214562 @default.
- W3183375372 cites W2337484140 @default.
- W3183375372 cites W2337946222 @default.
- W3183375372 cites W2409924309 @default.
- W3183375372 cites W2520365883 @default.
- W3183375372 cites W2527345731 @default.
- W3183375372 cites W2547373552 @default.
- W3183375372 cites W2562806882 @default.
- W3183375372 cites W2579817940 @default.
- W3183375372 cites W2757640349 @default.
- W3183375372 cites W2770462389 @default.
- W3183375372 cites W2772242482 @default.
- W3183375372 cites W2893463384 @default.
- W3183375372 cites W2911906719 @default.
- W3183375372 cites W2916721200 @default.
- W3183375372 cites W2976778560 @default.
- W3183375372 cites W2978809133 @default.
- W3183375372 cites W3008061540 @default.
- W3183375372 cites W3018336386 @default.
- W3183375372 cites W3045196858 @default.
- W3183375372 cites W40340874 @default.
- W3183375372 cites W419917605 @default.
- W3183375372 doi "https://doi.org/10.3390/toxins13070501" @default.
- W3183375372 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8310189" @default.
- W3183375372 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34357973" @default.
- W3183375372 hasPublicationYear "2021" @default.
- W3183375372 type Work @default.
- W3183375372 sameAs 3183375372 @default.
- W3183375372 citedByCount "4" @default.
- W3183375372 countsByYear W31833753722022 @default.
- W3183375372 countsByYear W31833753722023 @default.
- W3183375372 crossrefType "journal-article" @default.
- W3183375372 hasAuthorship W3183375372A5003678935 @default.
- W3183375372 hasAuthorship W3183375372A5016388201 @default.
- W3183375372 hasAuthorship W3183375372A5017965947 @default.
- W3183375372 hasAuthorship W3183375372A5029812885 @default.
- W3183375372 hasAuthorship W3183375372A5035195284 @default.
- W3183375372 hasAuthorship W3183375372A5056826659 @default.
- W3183375372 hasAuthorship W3183375372A5061968742 @default.
- W3183375372 hasAuthorship W3183375372A5062788088 @default.