FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3183417106> ?p ?o ?g. }

• W3183417106 abstract "Abstract Grasslands are widespread and economically relevant ecosystems at the basis of sustainable roughage production. Plant genetic diversity (PGD; i.e., within-species diversity) is related to many beneficial effects to the ecosystem functioning of grasslands. The monitoring of PGD in temperate grasslands is complicated by the multiplicity of species present and by a shortage of methods for large-scale assessment. However, the continuous advancement of high-throughput DNA sequencing approaches have improved the prospects of broad, multispecies PGD monitoring. Among them, amplicon sequencing stands out as a robust and cost-effective method. Here we report a set of twelve multispecies primer pairs that can be used for high-throughput PGD assessment in multiple grassland plant species. The loci targeted by the amplicons were selected and tested in two phases: a “discovery phase” based on a sequence capture assay (611 target nuclear loci assessed in 16 grassland plant species), which resulted in the selection of eleven loci; and a “validation phase”, in which the selected loci were targeted and sequenced using twelve multispecies primers in test populations of Dactylis glomerata L., Lolium perenne L., Festuca pratensis Huds., Trifolium pratense L. and T. repens L. The resulting multispecies amplicons had overall nucleotide diversities per species ranging from 5.19 × 10 −3 to 1.29 × 10 −2 , which is in the range of flowering-related genes but slightly lower than pathogen resistance genes. We conclude that the methodology, the DNA sequence resources, and the amplicon-specific primer pairs reported in this study provide the basis for large-scale, multispecies PGD monitoring in grassland plants." @default.
• W3183417106 created "2021-08-02" @default.
• W3183417106 creator A5029315294 @default.
• W3183417106 creator A5090669604 @default.
• W3183417106 creator A5090771221 @default.
• W3183417106 date "2021-07-26" @default.
• W3183417106 modified "2023-10-17" @default.
• W3183417106 title "A multispecies amplicon sequencing approach for genetic diversity assessment in grassland plant species" @default.
• W3183417106 cites W1181215895 @default.
• W3183417106 cites W1529325410 @default.
• W3183417106 cites W167520051 @default.
• W3183417106 cites W180025544 @default.
• W3183417106 cites W1972088832 @default.
• W3183417106 cites W1972171731 @default.
• W3183417106 cites W1977814320 @default.
• W3183417106 cites W1981624279 @default.
• W3183417106 cites W1991943452 @default.
• W3183417106 cites W1994145712 @default.
• W3183417106 cites W1997401532 @default.
• W3183417106 cites W2008856488 @default.
• W3183417106 cites W2011879824 @default.
• W3183417106 cites W2012986985 @default.
• W3183417106 cites W2021044098 @default.
• W3183417106 cites W2036897871 @default.
• W3183417106 cites W2053654058 @default.
• W3183417106 cites W2057215038 @default.
• W3183417106 cites W2086315836 @default.
• W3183417106 cites W2096128575 @default.
• W3183417106 cites W2096442157 @default.
• W3183417106 cites W2102619694 @default.
• W3183417106 cites W2107915254 @default.
• W3183417106 cites W2108234281 @default.
• W3183417106 cites W2120902911 @default.
• W3183417106 cites W2121955404 @default.
• W3183417106 cites W2127287497 @default.
• W3183417106 cites W2128605601 @default.
• W3183417106 cites W2135230723 @default.
• W3183417106 cites W2138098955 @default.
• W3183417106 cites W2149836365 @default.
• W3183417106 cites W2149992227 @default.
• W3183417106 cites W2150131459 @default.
• W3183417106 cites W2158093915 @default.
• W3183417106 cites W2303008992 @default.
• W3183417106 cites W2332186218 @default.
• W3183417106 cites W2341605457 @default.
• W3183417106 cites W2506089314 @default.
• W3183417106 cites W2607323900 @default.
• W3183417106 cites W2622731166 @default.
• W3183417106 cites W2751840108 @default.
• W3183417106 cites W2770565810 @default.
• W3183417106 cites W2786737018 @default.
• W3183417106 cites W2795542554 @default.
• W3183417106 cites W2896748444 @default.
• W3183417106 cites W2903368677 @default.
• W3183417106 cites W2909759141 @default.
• W3183417106 cites W2914169501 @default.
• W3183417106 cites W2943119887 @default.
• W3183417106 cites W2949448083 @default.
• W3183417106 cites W2950352437 @default.
• W3183417106 cites W2951890991 @default.
• W3183417106 cites W2951912016 @default.
• W3183417106 cites W2955329572 @default.
• W3183417106 cites W2974278100 @default.
• W3183417106 cites W2994955965 @default.
• W3183417106 cites W3002211279 @default.
• W3183417106 cites W3005222101 @default.
• W3183417106 cites W3006233966 @default.
• W3183417106 cites W3009635362 @default.
• W3183417106 cites W3016075651 @default.
• W3183417106 cites W3038412545 @default.
• W3183417106 cites W3121953277 @default.
• W3183417106 cites W3168947834 @default.
• W3183417106 doi "https://doi.org/10.1101/2021.07.26.453819" @default.
• W3183417106 hasPublicationYear "2021" @default.
• W3183417106 type Work @default.
• W3183417106 sameAs 3183417106 @default.
• W3183417106 citedByCount "0" @default.
• W3183417106 crossrefType "posted-content" @default.
• W3183417106 hasAuthorship W3183417106A5029315294 @default.
• W3183417106 hasAuthorship W3183417106A5090669604 @default.
• W3183417106 hasAuthorship W3183417106A5090771221 @default.
• W3183417106 hasBestOaLocation W31834171061 @default.
• W3183417106 hasConcept C104317684 @default.
• W3183417106 hasConcept C127937792 @default.
• W3183417106 hasConcept C144024400 @default.
• W3183417106 hasConcept C149923435 @default.
• W3183417106 hasConcept C18903297 @default.
• W3183417106 hasConcept C2778659099 @default.
• W3183417106 hasConcept C2908647359 @default.
• W3183417106 hasConcept C46757340 @default.
• W3183417106 hasConcept C49105822 @default.
• W3183417106 hasConcept C54355233 @default.
• W3183417106 hasConcept C62648534 @default.
• W3183417106 hasConcept C8185291 @default.
• W3183417106 hasConcept C81977670 @default.
• W3183417106 hasConcept C86803240 @default.
• W3183417106 hasConceptScore W3183417106C104317684 @default.
• W3183417106 hasConceptScore W3183417106C127937792 @default.
• W3183417106 hasConceptScore W3183417106C144024400 @default.
• W3183417106 hasConceptScore W3183417106C149923435 @default.

• next