FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3183618565> ?p ?o ?g. }

• W3183618565 endingPage "8129" @default.
• W3183618565 startingPage "8129" @default.
• W3183618565 abstract "Background: Type 2 diabetes mellitus is one of the leading causes of morbidity and mortality worldwide and is derived from an accumulation of genetic and epigenetic changes. In this study, we aimed to construct Insilco, a competing endogenous RNA (ceRNA) network linked to the pathogenesis of insulin resistance followed by its experimental validation in patients’, matched control and cell line samples, as well as to evaluate the efficacy of CRISPR/Cas9 as a potential therapeutic strategy to modulate the expression of this deregulated network. By applying bioinformatics tools through a two-step process, we identified and verified a ceRNA network panel of mRNAs, miRNAs and lncRNA related to insulin resistance, Then validated the expression in clinical samples (123 patients and 106 controls) and some of matched cell line samples using real time PCR. Next, two guide RNAs were designed to target the sequence flanking LncRNA/miRNAs interaction by CRISPER/Cas9 in cell culture. Gene editing tool efficacy was assessed by measuring the network downstream proteins GLUT4 and mTOR via immunofluorescence. Results: LncRNA-RP11-773H22.4, together with RET, IGF1R and mTOR mRNAs, showed significant upregulation in T2DM compared with matched controls, while miRNA (i.e., miR-3163 and miR-1) and mRNA (i.e., GLUT4 and AKT2) expression displayed marked downregulation in diabetic samples. CRISPR/Cas9 successfully knocked out LncRNA-RP11-773H22.4, as evidenced by the reversal of the gene expression of the identified network at RNA and protein levels to the normal expression pattern after gene editing. Conclusions: The present study provides the significance of this ceRNA based network and its related target genes panel both in the pathogenesis of insulin resistance and as a therapeutic target for gene editing in T2DM." @default.
• W3183618565 created "2021-08-02" @default.
• W3183618565 creator A5001885273 @default.
• W3183618565 creator A5004237763 @default.
• W3183618565 creator A5014166060 @default.
• W3183618565 creator A5026455065 @default.
• W3183618565 creator A5040018396 @default.
• W3183618565 creator A5053498117 @default.
• W3183618565 creator A5063132082 @default.
• W3183618565 date "2021-07-29" @default.
• W3183618565 modified "2023-09-27" @default.
• W3183618565 title "Identification of Novel Insulin Resistance Related ceRNA Network in T2DM and Its Potential Editing by CRISPR/Cas9" @default.
• W3183618565 cites W1929556414 @default.
• W3183618565 cites W2047385027 @default.
• W3183618565 cites W2053073281 @default.
• W3183618565 cites W2067589497 @default.
• W3183618565 cites W2079776805 @default.
• W3183618565 cites W2092817365 @default.
• W3183618565 cites W2096286013 @default.
• W3183618565 cites W2105153491 @default.
• W3183618565 cites W2160234571 @default.
• W3183618565 cites W2168420558 @default.
• W3183618565 cites W2176255241 @default.
• W3183618565 cites W2211649130 @default.
• W3183618565 cites W2257452859 @default.
• W3183618565 cites W2263766349 @default.
• W3183618565 cites W2286654631 @default.
• W3183618565 cites W2337024486 @default.
• W3183618565 cites W2345838610 @default.
• W3183618565 cites W2511664109 @default.
• W3183618565 cites W2566748320 @default.
• W3183618565 cites W2571274939 @default.
• W3183618565 cites W2578649298 @default.
• W3183618565 cites W2589290067 @default.
• W3183618565 cites W2602590693 @default.
• W3183618565 cites W2691416952 @default.
• W3183618565 cites W2729245689 @default.
• W3183618565 cites W2783270596 @default.
• W3183618565 cites W2888130072 @default.
• W3183618565 cites W2898425821 @default.
• W3183618565 cites W2900914388 @default.
• W3183618565 cites W2901045644 @default.
• W3183618565 cites W2901289306 @default.
• W3183618565 cites W2980412861 @default.
• W3183618565 cites W2986494140 @default.
• W3183618565 doi "https://doi.org/10.3390/ijms22158129" @default.
• W3183618565 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8348752" @default.
• W3183618565 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34360895" @default.
• W3183618565 hasPublicationYear "2021" @default.
• W3183618565 type Work @default.
• W3183618565 sameAs 3183618565 @default.
• W3183618565 citedByCount "4" @default.
• W3183618565 countsByYear W31836185652022 @default.
• W3183618565 countsByYear W31836185652023 @default.
• W3183618565 crossrefType "journal-article" @default.
• W3183618565 hasAuthorship W3183618565A5001885273 @default.
• W3183618565 hasAuthorship W3183618565A5004237763 @default.
• W3183618565 hasAuthorship W3183618565A5014166060 @default.
• W3183618565 hasAuthorship W3183618565A5026455065 @default.
• W3183618565 hasAuthorship W3183618565A5040018396 @default.
• W3183618565 hasAuthorship W3183618565A5053498117 @default.
• W3183618565 hasAuthorship W3183618565A5063132082 @default.
• W3183618565 hasBestOaLocation W31836185651 @default.
• W3183618565 hasConcept C104317684 @default.
• W3183618565 hasConcept C127561419 @default.
• W3183618565 hasConcept C132455925 @default.
• W3183618565 hasConcept C145059251 @default.
• W3183618565 hasConcept C54355233 @default.
• W3183618565 hasConcept C60365752 @default.
• W3183618565 hasConcept C62203573 @default.
• W3183618565 hasConcept C62478195 @default.
• W3183618565 hasConcept C70721500 @default.
• W3183618565 hasConcept C86554907 @default.
• W3183618565 hasConcept C86803240 @default.
• W3183618565 hasConcept C98108389 @default.
• W3183618565 hasConceptScore W3183618565C104317684 @default.
• W3183618565 hasConceptScore W3183618565C127561419 @default.
• W3183618565 hasConceptScore W3183618565C132455925 @default.
• W3183618565 hasConceptScore W3183618565C145059251 @default.
• W3183618565 hasConceptScore W3183618565C54355233 @default.
• W3183618565 hasConceptScore W3183618565C60365752 @default.
• W3183618565 hasConceptScore W3183618565C62203573 @default.
• W3183618565 hasConceptScore W3183618565C62478195 @default.
• W3183618565 hasConceptScore W3183618565C70721500 @default.
• W3183618565 hasConceptScore W3183618565C86554907 @default.
• W3183618565 hasConceptScore W3183618565C86803240 @default.
• W3183618565 hasConceptScore W3183618565C98108389 @default.
• W3183618565 hasIssue "15" @default.
• W3183618565 hasLocation W31836185651 @default.
• W3183618565 hasLocation W31836185652 @default.
• W3183618565 hasOpenAccess W3183618565 @default.
• W3183618565 hasPrimaryLocation W31836185651 @default.
• W3183618565 hasRelatedWork W2160068229 @default.
• W3183618565 hasRelatedWork W2401645900 @default.
• W3183618565 hasRelatedWork W2557582719 @default.
• W3183618565 hasRelatedWork W2742734776 @default.
• W3183618565 hasRelatedWork W2790324218 @default.
• W3183618565 hasRelatedWork W2946365774 @default.

• next