FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3183993759> ?p ?o ?g. }

• W3183993759 endingPage "10" @default.
• W3183993759 startingPage "10" @default.
• W3183993759 abstract "Purpose: Repair of distal phalanx amputation is widely studied and thought to occur through a regenerative process. Single-cell RNA sequencing (scRNA) has greatly improved the ability to characterize the cells that are involved in regeneration and abnormal healing of bone and soft tissue. Given the disorganized architecture of digit tip “regenerated” bone and its common progenitor cell to the pathologic process of heterotopic ossification, we hypothesized that digit tip repair after injury follows a program of aberrant wound repair rather than true regeneration of normal bone tissue. Methods: ScRNA data of mouse digit tip amputation (DTA) and embryonic limb development (EMB) was downloaded from NBCI GEO data base ascension number GSE135985. Additionally, scRNA data from heterotopic ossification (HO) was downloaded from GSE126060. HO, DTA, and EMB datasets were clustered individually. HO was merged and aligned with EMB and DT in separate analyses. Cells expressing the mesenchymal progenitor cell (MPC) marker Pdgfra, which we have shown to differentiate into HO and others have shown to differentiate into bone after DTA, were identified and analyzed in the HO-DTA and HO-EMB datasets. Monocle 2, Seurat 3.1.1, and Pearson correlation analysis were used for analysis and visualization. Immunostaining for markers present in DTA (ACAN, FBN-2, LTBP2) was performed on sections collected from a murine model of traumatic HO in Pdgfra-CreER;TdTomato mice induced with tamoxifen one-week prior to HO-forming injury. Results: MPC clusters from DTA and HO and from EMB and HO were aligned and projected using UMAP to compare transcription profiles. Across the timepoints, MPCs in the DTA and HO occupy similar regions of the UMAP plot, while MPCs in EMB and HO occupy largely independent regions, with only overlap at the latest time points. Using Monocle 2, cells were placed on a virtual timeline based on similarities in transcription. MPCs from DTA and HO meet at the same location on the trajectory following injury. In the EMB to HO comparison, cells only occupy similar regions of the trajectory at post-natal day 3 suggesting that bone is formed by different pathways. To understand correlations between time points, transcriptomes were also compared by Pearson analysis. MPCs following HO and DTA injuries occupy similar regions of the plot while MPCs in EMB and HO only occupy similar regions of the correlation plot at P3. Markers previously identified to be upregulated in MPCs in DTA were also upregulated in MPCs in HO by both scRNA and immunofluorescent histology. Conclusions: Cells responsible for bone repair following DTA show similar transcriptional profiles as MPCs in traumatic HO and dissimilar to MPCs in EMB. Furthermore, MPCs in both digit tip repair and HO follow trajectories that do not overlap with embryonic limb development. Thus, we demonstrate for the first time, that the process following DTA is not truly regeneration and follows programs of aberrant repair as seen in HO." @default.
• W3183993759 created "2021-08-02" @default.
• W3183993759 creator A5001867555 @default.
• W3183993759 creator A5007300259 @default.
• W3183993759 creator A5013159769 @default.
• W3183993759 creator A5019742336 @default.
• W3183993759 creator A5040637100 @default.
• W3183993759 creator A5041777639 @default.
• W3183993759 creator A5048877907 @default.
• W3183993759 creator A5064874339 @default.
• W3183993759 creator A5085384148 @default.
• W3183993759 date "2021-07-01" @default.
• W3183993759 modified "2023-10-17" @default.
• W3183993759 title "QS7: Digit Tip Regeneration Following Amputation Is Not True Regeneration" @default.
• W3183993759 doi "https://doi.org/10.1097/01.gox.0000769976.55775.fc" @default.
• W3183993759 hasPublicationYear "2021" @default.
• W3183993759 type Work @default.
• W3183993759 sameAs 3183993759 @default.
• W3183993759 citedByCount "0" @default.
• W3183993759 crossrefType "journal-article" @default.
• W3183993759 hasAuthorship W3183993759A5001867555 @default.
• W3183993759 hasAuthorship W3183993759A5007300259 @default.
• W3183993759 hasAuthorship W3183993759A5013159769 @default.
• W3183993759 hasAuthorship W3183993759A5019742336 @default.
• W3183993759 hasAuthorship W3183993759A5040637100 @default.
• W3183993759 hasAuthorship W3183993759A5041777639 @default.
• W3183993759 hasAuthorship W3183993759A5048877907 @default.
• W3183993759 hasAuthorship W3183993759A5064874339 @default.
• W3183993759 hasAuthorship W3183993759A5085384148 @default.
• W3183993759 hasBestOaLocation W31839937591 @default.
• W3183993759 hasConcept C105702510 @default.
• W3183993759 hasConcept C141071460 @default.
• W3183993759 hasConcept C142724271 @default.
• W3183993759 hasConcept C171056886 @default.
• W3183993759 hasConcept C2776204877 @default.
• W3183993759 hasConcept C2780650075 @default.
• W3183993759 hasConcept C71924100 @default.
• W3183993759 hasConcept C86803240 @default.
• W3183993759 hasConcept C95444343 @default.
• W3183993759 hasConceptScore W3183993759C105702510 @default.
• W3183993759 hasConceptScore W3183993759C141071460 @default.
• W3183993759 hasConceptScore W3183993759C142724271 @default.
• W3183993759 hasConceptScore W3183993759C171056886 @default.
• W3183993759 hasConceptScore W3183993759C2776204877 @default.
• W3183993759 hasConceptScore W3183993759C2780650075 @default.
• W3183993759 hasConceptScore W3183993759C71924100 @default.
• W3183993759 hasConceptScore W3183993759C86803240 @default.
• W3183993759 hasConceptScore W3183993759C95444343 @default.
• W3183993759 hasIssue "7S" @default.
• W3183993759 hasLocation W31839937591 @default.
• W3183993759 hasLocation W31839937592 @default.
• W3183993759 hasLocation W31839937593 @default.
• W3183993759 hasLocation W31839937594 @default.
• W3183993759 hasOpenAccess W3183993759 @default.
• W3183993759 hasPrimaryLocation W31839937591 @default.
• W3183993759 hasRelatedWork W1971492634 @default.
• W3183993759 hasRelatedWork W2007160459 @default.
• W3183993759 hasRelatedWork W2008518697 @default.
• W3183993759 hasRelatedWork W2074767636 @default.
• W3183993759 hasRelatedWork W2243300080 @default.
• W3183993759 hasRelatedWork W2409420033 @default.
• W3183993759 hasRelatedWork W2914253411 @default.
• W3183993759 hasRelatedWork W2946489133 @default.
• W3183993759 hasRelatedWork W4205643535 @default.
• W3183993759 hasRelatedWork W4288392678 @default.
• W3183993759 hasVolume "9" @default.
• W3183993759 isParatext "false" @default.
• W3183993759 isRetracted "false" @default.
• W3183993759 magId "3183993759" @default.
• W3183993759 workType "article" @default.