FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3184227730> ?p ?o ?g. }

• W3184227730 endingPage "100081" @default.
• W3184227730 startingPage "100081" @default.
• W3184227730 abstract "Syndecans are membrane proteoglycans regulating extracellular matrix assembly, cell adhesion and signaling. Their ectodomains can be shed from the cell surface, and act as paracrine and autocrine effectors or as competitors of full-length syndecans. We report the first biophysical characterization of the recombinant ectodomains of the four human syndecans using biophysical techniques, and show that they behave like flexible random-coil intrinsically disordered proteins, and adopt several conformation ensembles in solution. We have characterized their conformational landscapes using native mass spectrometry (MS) and ion-mobility MS, and demonstrated that the syndecan ectodomains explore the majority of their conformational landscape, from minor compact, globular-like, conformations to extended ones. We also report that the ectodomain of syndecan-4, corresponding to a natural isoform, is able to dimerize via a disulfide bond. We have generated a three-dimensional model of the C-terminus of this dimer, which supports the dimerization via a disulfide bond. Furthermore, we have mapped the NXIP adhesion motif of syndecans and their sequences involved in the formation of ternary complexes with integrins and growth factor receptors on the major conformations of their ectodomains, and shown that these sequences are not accessible in all the conformations, suggesting that only some of them are biologically active. Lastly, although the syndecan ectodomains have a far lower number of amino acid residues than their membrane partners, their intrinsic disorder and flexibility allow them to adopt extended conformations, which have roughly the same size as the cell surface receptors (e.g., integrins and growth factor receptors) they bind to." @default.
• W3184227730 created "2021-08-02" @default.
• W3184227730 creator A5001173203 @default.
• W3184227730 creator A5005054796 @default.
• W3184227730 creator A5016715495 @default.
• W3184227730 creator A5035188240 @default.
• W3184227730 creator A5052827727 @default.
• W3184227730 creator A5055191235 @default.
• W3184227730 creator A5084156210 @default.
• W3184227730 creator A5091852812 @default.
• W3184227730 date "2021-12-01" @default.
• W3184227730 modified "2023-10-01" @default.
• W3184227730 title "Extended disorder at the cell surface: The conformational landscape of the ectodomains of syndecans" @default.
• W3184227730 cites W1131036567 @default.
• W3184227730 cites W1554961657 @default.
• W3184227730 cites W1593371871 @default.
• W3184227730 cites W1872803365 @default.
• W3184227730 cites W1889990950 @default.
• W3184227730 cites W1929415106 @default.
• W3184227730 cites W1954141448 @default.
• W3184227730 cites W1973815785 @default.
• W3184227730 cites W1977591985 @default.
• W3184227730 cites W1981346588 @default.
• W3184227730 cites W1993440705 @default.
• W3184227730 cites W1994731124 @default.
• W3184227730 cites W1997441326 @default.
• W3184227730 cites W2002448780 @default.
• W3184227730 cites W2003543603 @default.
• W3184227730 cites W2015660202 @default.
• W3184227730 cites W2026727321 @default.
• W3184227730 cites W2043176704 @default.
• W3184227730 cites W2045529078 @default.
• W3184227730 cites W2046287218 @default.
• W3184227730 cites W2049747252 @default.
• W3184227730 cites W2051352934 @default.
• W3184227730 cites W2057103288 @default.
• W3184227730 cites W2065735171 @default.
• W3184227730 cites W2066158357 @default.
• W3184227730 cites W2077199821 @default.
• W3184227730 cites W2079134493 @default.
• W3184227730 cites W2087356589 @default.
• W3184227730 cites W2087638674 @default.
• W3184227730 cites W2088267142 @default.
• W3184227730 cites W2093894241 @default.
• W3184227730 cites W2101137168 @default.
• W3184227730 cites W2104966864 @default.
• W3184227730 cites W2146074826 @default.
• W3184227730 cites W2148684261 @default.
• W3184227730 cites W2149789639 @default.
• W3184227730 cites W2155039674 @default.
• W3184227730 cites W2156981779 @default.
• W3184227730 cites W2158081651 @default.
• W3184227730 cites W2166981099 @default.
• W3184227730 cites W2170146374 @default.
• W3184227730 cites W2256940081 @default.
• W3184227730 cites W2279384388 @default.
• W3184227730 cites W2290519997 @default.
• W3184227730 cites W2416801748 @default.
• W3184227730 cites W2440193997 @default.
• W3184227730 cites W2473775182 @default.
• W3184227730 cites W2474143322 @default.
• W3184227730 cites W2518625155 @default.
• W3184227730 cites W2530994728 @default.
• W3184227730 cites W2546451980 @default.
• W3184227730 cites W2558396410 @default.
• W3184227730 cites W2620357404 @default.
• W3184227730 cites W2707646777 @default.
• W3184227730 cites W2764087674 @default.
• W3184227730 cites W2790223572 @default.
• W3184227730 cites W2791942866 @default.
• W3184227730 cites W2802007263 @default.
• W3184227730 cites W2898567411 @default.
• W3184227730 cites W2898755565 @default.
• W3184227730 cites W2911426481 @default.
• W3184227730 cites W2912126500 @default.
• W3184227730 cites W2933738970 @default.
• W3184227730 cites W2938997475 @default.
• W3184227730 cites W2950020601 @default.
• W3184227730 cites W2973222431 @default.
• W3184227730 cites W2977366709 @default.
• W3184227730 cites W2986689826 @default.
• W3184227730 cites W3003929865 @default.
• W3184227730 cites W3112873140 @default.
• W3184227730 doi "https://doi.org/10.1016/j.mbplus.2021.100081" @default.
• W3184227730 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8416954" @default.
• W3184227730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34505054" @default.
• W3184227730 hasPublicationYear "2021" @default.
• W3184227730 type Work @default.
• W3184227730 sameAs 3184227730 @default.
• W3184227730 citedByCount "6" @default.
• W3184227730 countsByYear W31842277302022 @default.
• W3184227730 countsByYear W31842277302023 @default.
• W3184227730 crossrefType "journal-article" @default.
• W3184227730 hasAuthorship W3184227730A5001173203 @default.
• W3184227730 hasAuthorship W3184227730A5005054796 @default.
• W3184227730 hasAuthorship W3184227730A5016715495 @default.
• W3184227730 hasAuthorship W3184227730A5035188240 @default.
• W3184227730 hasAuthorship W3184227730A5052827727 @default.

• next