FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3184675023> ?p ?o ?g. }

• W3184675023 abstract "Abstract DNA–protein interactions are essential for several molecular and cellular mechanisms, such as transcription, transcriptional regulation, DNA modifications, among others. For many decades scientists tried to unravel how DNA links to proteins, forming complex and vital interactions. However, the high number of techniques developed for the study of these interactions made the choice of the appropriate technique a difficult task. This review intends to provide a historical context and compile the methods that describe DNA–protein interactions according to the purpose of each approach, summarise the respective advantages and disadvantages and give some examples of recent uses for each technique. The final aim of this work is to help in deciding which technique to perform according to the objectives and capacities of each research team. Considering the DNA–binding proteins characterisation, filter binding assay and EMSA are easy in vitro methods that rapidly identify nucleic acid-protein binding interactions. To find DNA-binding sites, DNA-footprinting is indeed an easier, faster and reliable approach, however, techniques involving base analogues and base-site selection are more precise. Concerning binding kinetics and affinities, filter binding assay and EMSA are useful and easy methods, although SPR and spectroscopy techniques are more sensitive. Finally, relatively to genome-wide studies, ChIP–seq is the desired method, given the coverage and resolution of the technique. In conclusion, although some experiments are easier and faster than others, when designing a DNA–protein interaction study several concerns should be taken and different techniques may need to be considered, since different methods confer different precisions and accuracies." @default.
• W3184675023 created "2021-08-02" @default.
• W3184675023 creator A5018745231 @default.
• W3184675023 creator A5027903454 @default.
• W3184675023 creator A5048188883 @default.
• W3184675023 creator A5064465806 @default.
• W3184675023 creator A5080862661 @default.
• W3184675023 creator A5082162292 @default.
• W3184675023 date "2021-07-23" @default.
• W3184675023 modified "2023-10-16" @default.
• W3184675023 title "DNA–protein interaction studies: a historical and comparative analysis" @default.
• W3184675023 cites W1480111241 @default.
• W3184675023 cites W1528944186 @default.
• W3184675023 cites W1573008071 @default.
• W3184675023 cites W1965267927 @default.
• W3184675023 cites W1967465227 @default.
• W3184675023 cites W1976260744 @default.
• W3184675023 cites W1979871091 @default.
• W3184675023 cites W1985468796 @default.
• W3184675023 cites W1986352563 @default.
• W3184675023 cites W1986387313 @default.
• W3184675023 cites W1986586522 @default.
• W3184675023 cites W1986941949 @default.
• W3184675023 cites W1986993156 @default.
• W3184675023 cites W1989419347 @default.
• W3184675023 cites W1994795223 @default.
• W3184675023 cites W1995703134 @default.
• W3184675023 cites W1997641930 @default.
• W3184675023 cites W1998242163 @default.
• W3184675023 cites W1999219397 @default.
• W3184675023 cites W2002017151 @default.
• W3184675023 cites W2004563262 @default.
• W3184675023 cites W2005223383 @default.
• W3184675023 cites W2009819259 @default.
• W3184675023 cites W2011509827 @default.
• W3184675023 cites W2017927310 @default.
• W3184675023 cites W2019970315 @default.
• W3184675023 cites W2021563901 @default.
• W3184675023 cites W2022102345 @default.
• W3184675023 cites W2024462893 @default.
• W3184675023 cites W2032619610 @default.
• W3184675023 cites W2033744382 @default.
• W3184675023 cites W2038003996 @default.
• W3184675023 cites W2040907350 @default.
• W3184675023 cites W2040982403 @default.
• W3184675023 cites W2045104360 @default.
• W3184675023 cites W2045757118 @default.
• W3184675023 cites W2052821301 @default.
• W3184675023 cites W2058726558 @default.
• W3184675023 cites W2059775890 @default.
• W3184675023 cites W2065236128 @default.
• W3184675023 cites W2065484514 @default.
• W3184675023 cites W2066706759 @default.
• W3184675023 cites W2071492597 @default.
• W3184675023 cites W2071566849 @default.
• W3184675023 cites W2072897152 @default.
• W3184675023 cites W2079321730 @default.
• W3184675023 cites W2079450159 @default.
• W3184675023 cites W2079461029 @default.
• W3184675023 cites W2089273635 @default.
• W3184675023 cites W2091041044 @default.
• W3184675023 cites W2092988184 @default.
• W3184675023 cites W2094392998 @default.
• W3184675023 cites W2095042450 @default.
• W3184675023 cites W2095096192 @default.
• W3184675023 cites W2101108802 @default.
• W3184675023 cites W2102154371 @default.
• W3184675023 cites W2102369193 @default.
• W3184675023 cites W2106757431 @default.
• W3184675023 cites W2123631149 @default.
• W3184675023 cites W2128533758 @default.
• W3184675023 cites W2129795133 @default.
• W3184675023 cites W2133188555 @default.
• W3184675023 cites W2134228306 @default.
• W3184675023 cites W2137154596 @default.
• W3184675023 cites W2142244017 @default.
• W3184675023 cites W2153530880 @default.
• W3184675023 cites W2159431004 @default.
• W3184675023 cites W2170836338 @default.
• W3184675023 cites W2171111703 @default.
• W3184675023 cites W2171407623 @default.
• W3184675023 cites W2410634198 @default.
• W3184675023 cites W2429556402 @default.
• W3184675023 cites W2576734941 @default.
• W3184675023 cites W2742195959 @default.
• W3184675023 cites W2801340345 @default.
• W3184675023 cites W2924068395 @default.
• W3184675023 cites W3021687800 @default.
• W3184675023 cites W3040879296 @default.
• W3184675023 cites W3090418754 @default.
• W3184675023 cites W3112842714 @default.
• W3184675023 cites W4234153398 @default.
• W3184675023 cites W4248318091 @default.
• W3184675023 doi "https://doi.org/10.1186/s13007-021-00780-z" @default.
• W3184675023 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8299673" @default.
• W3184675023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34301293" @default.
• W3184675023 hasPublicationYear "2021" @default.
• W3184675023 type Work @default.
• W3184675023 sameAs 3184675023 @default.
• W3184675023 citedByCount "20" @default.

• next