FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3184802234> ?p ?o ?g. }

• W3184802234 endingPage "10461" @default.
• W3184802234 startingPage "10453" @default.
• W3184802234 abstract "The ability to noninvasively monitor stem cells' differentiation is important to stem cell studies. Raman spectroscopy is a non-harmful imaging approach that acquires the cellular biochemical signatures. Herein, we report the first use of label-free Raman spectroscopy to characterize the gradual change during the differentiation process of live human neural stem cells (NSCs) in the in vitro cultures. Raman spectra of 600-1800 cm-1 were measured with human NSC cultures from the undifferentiated stage (NSC-predominant) to the highly differentiated one (neuron-predominant) and subsequently analyzed using various mathematical methods. Hierarchical cluster analysis distinguished two cell types (NSCs and neurons) through the spectra. The subsequently derived differentiation rate matched that measured by immunocytochemistry. The key spectral biomarkers were identified by time-dependent trend analysis and principal component analysis. Furthermore, through machine learning-based analysis, a set of eight spectral data points were found to be highly accurate in classifying cell types and predicting the differentiation rate. The predictive accuracy was the highest using the artificial neural network (ANN) and slightly lowered using the logistic regression model and linear discriminant analysis. In conclusion, label-free Raman spectroscopy with the aid of machine learning analysis can provide the noninvasive classification of cell types at the single-cell level and thus accurately track the human NSC differentiation. A set of eight spectral data points combined with the ANN method were found to be the most efficient and accurate. Establishing this non-harmful and efficient strategy will shed light on the in vivo and clinical studies of NSCs." @default.
• W3184802234 created "2021-08-02" @default.
• W3184802234 creator A5015117536 @default.
• W3184802234 creator A5021356457 @default.
• W3184802234 creator A5050852420 @default.
• W3184802234 creator A5064548129 @default.
• W3184802234 creator A5076699095 @default.
• W3184802234 creator A5078066309 @default.
• W3184802234 date "2021-07-20" @default.
• W3184802234 modified "2023-10-03" @default.
• W3184802234 title "Tracking the Differentiation Status of Human Neural Stem Cells through Label-Free Raman Spectroscopy and Machine Learning-Based Analysis" @default.
• W3184802234 cites W1504471840 @default.
• W3184802234 cites W1592082209 @default.
• W3184802234 cites W1644332254 @default.
• W3184802234 cites W1722301188 @default.
• W3184802234 cites W1766315665 @default.
• W3184802234 cites W1966789592 @default.
• W3184802234 cites W1968092893 @default.
• W3184802234 cites W1973194576 @default.
• W3184802234 cites W1973898642 @default.
• W3184802234 cites W1975391983 @default.
• W3184802234 cites W1977058403 @default.
• W3184802234 cites W1979274415 @default.
• W3184802234 cites W1980505853 @default.
• W3184802234 cites W1980721182 @default.
• W3184802234 cites W1984870807 @default.
• W3184802234 cites W1985082107 @default.
• W3184802234 cites W1991848485 @default.
• W3184802234 cites W1992365936 @default.
• W3184802234 cites W1992924696 @default.
• W3184802234 cites W2001255811 @default.
• W3184802234 cites W2006838165 @default.
• W3184802234 cites W2014158190 @default.
• W3184802234 cites W2018726462 @default.
• W3184802234 cites W2019986565 @default.
• W3184802234 cites W2028542248 @default.
• W3184802234 cites W2028781440 @default.
• W3184802234 cites W2030252423 @default.
• W3184802234 cites W2035421409 @default.
• W3184802234 cites W2043938008 @default.
• W3184802234 cites W2052940553 @default.
• W3184802234 cites W2063698105 @default.
• W3184802234 cites W2066477553 @default.
• W3184802234 cites W2071060299 @default.
• W3184802234 cites W2073523882 @default.
• W3184802234 cites W2074000348 @default.
• W3184802234 cites W2091784906 @default.
• W3184802234 cites W2093613748 @default.
• W3184802234 cites W2099182595 @default.
• W3184802234 cites W2105563927 @default.
• W3184802234 cites W2110076945 @default.
• W3184802234 cites W2113004955 @default.
• W3184802234 cites W2113047113 @default.
• W3184802234 cites W2122612492 @default.
• W3184802234 cites W2132312679 @default.
• W3184802234 cites W2135295279 @default.
• W3184802234 cites W2135846857 @default.
• W3184802234 cites W2146884475 @default.
• W3184802234 cites W2154743597 @default.
• W3184802234 cites W2157367129 @default.
• W3184802234 cites W2159711399 @default.
• W3184802234 cites W2160377357 @default.
• W3184802234 cites W2163326942 @default.
• W3184802234 cites W2403859484 @default.
• W3184802234 cites W2473793589 @default.
• W3184802234 cites W2522745591 @default.
• W3184802234 cites W2784183806 @default.
• W3184802234 cites W2791478484 @default.
• W3184802234 cites W2885560353 @default.
• W3184802234 cites W2889007696 @default.
• W3184802234 cites W2912183668 @default.
• W3184802234 cites W2924592037 @default.
• W3184802234 cites W2964922465 @default.
• W3184802234 cites W2999277653 @default.
• W3184802234 cites W3041858956 @default.
• W3184802234 cites W3044577971 @default.
• W3184802234 cites W3088836360 @default.
• W3184802234 cites W3093993828 @default.
• W3184802234 cites W3096858804 @default.
• W3184802234 cites W3103321707 @default.
• W3184802234 cites W4238768614 @default.
• W3184802234 doi "https://doi.org/10.1021/acs.analchem.0c04941" @default.
• W3184802234 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34282890" @default.
• W3184802234 hasPublicationYear "2021" @default.
• W3184802234 type Work @default.
• W3184802234 sameAs 3184802234 @default.
• W3184802234 citedByCount "11" @default.
• W3184802234 countsByYear W31848022342022 @default.
• W3184802234 countsByYear W31848022342023 @default.
• W3184802234 crossrefType "journal-article" @default.
• W3184802234 hasAuthorship W3184802234A5015117536 @default.
• W3184802234 hasAuthorship W3184802234A5021356457 @default.
• W3184802234 hasAuthorship W3184802234A5050852420 @default.
• W3184802234 hasAuthorship W3184802234A5064548129 @default.
• W3184802234 hasAuthorship W3184802234A5076699095 @default.
• W3184802234 hasAuthorship W3184802234A5078066309 @default.
• W3184802234 hasConcept C119857082 @default.
• W3184802234 hasConcept C120665830 @default.

• next