Matches in SemOpenAlex for { <https://semopenalex.org/work/W3184879324> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W3184879324 endingPage "282" @default.
- W3184879324 startingPage "276" @default.
- W3184879324 abstract "Aim. To determine the role of the acetate in the persistence of indigenous bifidobacteria in the colon biotope through the lysozyme resistance in model conditions of the acetylation–deacetylation of peptidoglycan.Materials and methods. The study was performed on 16 strains of the two indigenous bifidobacteria speсies: Bifidobacterium bifidum и Bifidobacterium longum subsp. longum. Bifidobacteria was cultivated in the 0.6% O2 and 9% CO2 atmosphere at the temperature 37ºС in CO2 incubator for 48 hours. The production of the acetate by the bifidobacteria was determined by gas chromatography. The effect of the acetate on the lysozyme resistance of non-indigenous gram-positive bacteria was determined on the Listeria monocytogenes ICIS-280 model strain by the cultivation in LB-Lennox broth with ammonium acetate added in the concentration range matching the concentrations produced by the studied bifidobacteria, in lysozyme serial dilutions at final concentrations 5 μg/ml to 40 μg/ml within 24 hours.Results. It was found that the acetate release of Bifidobacterium longum subsp. longum was on average two times higher that of Bifidobacterium bifidum (27.0 and 14.7 mmol/liter, respectively) and was quite consistent with the concentrations of acetic acid determined in the intestinal contents (up to 50 mmol/liter). Cultivation of bifidobacteria in a medium with lysozyme, ammonium acetate and their combination did not have a significant impact on their growth parameters at the maximum used concentrations of these substances. In the test strain, the addition of ammonium acetate in the range created by bifidobacteria caused a decrease in the minimum inhibitory concentration of lysozyme by more than two times — from 40 μg/ml to less than 20 μg/ml. In the control medium without lysozyme, no inhibition of the growth of the indicator culture was observed up to the maximum concentrations of ammonium acetate.Conclusion. The mechanism of persistence (survival) of indigenous bifidobacteria in the human intestinal biotope has been identified, which is associated with the production of acetic acid at a level that selectively suppresses lysozyme resistance of non-indigenous gram-positive microbiota viareversible deacetylation of peptidoglycan. This allows indigenous bifidobacteria to maintain a stable dominant position in the biotope." @default.
- W3184879324 created "2021-08-02" @default.
- W3184879324 creator A5008333211 @default.
- W3184879324 creator A5024231734 @default.
- W3184879324 creator A5060585538 @default.
- W3184879324 creator A5072311034 @default.
- W3184879324 date "2021-07-01" @default.
- W3184879324 modified "2023-10-12" @default.
- W3184879324 title "Mechanism of persistence of indigenous bifidobacteria under the impact of acetate in the human colon biotope" @default.
- W3184879324 cites W1551752370 @default.
- W3184879324 cites W1974603596 @default.
- W3184879324 cites W2052779789 @default.
- W3184879324 cites W2157262295 @default.
- W3184879324 cites W2162392176 @default.
- W3184879324 cites W2162717091 @default.
- W3184879324 cites W2164424979 @default.
- W3184879324 cites W2471122499 @default.
- W3184879324 cites W2611008897 @default.
- W3184879324 cites W2793871441 @default.
- W3184879324 cites W2953817430 @default.
- W3184879324 cites W2966940631 @default.
- W3184879324 doi "https://doi.org/10.36233/0372-9311-86" @default.
- W3184879324 hasPublicationYear "2021" @default.
- W3184879324 type Work @default.
- W3184879324 sameAs 3184879324 @default.
- W3184879324 citedByCount "3" @default.
- W3184879324 countsByYear W31848793242022 @default.
- W3184879324 crossrefType "journal-article" @default.
- W3184879324 hasAuthorship W3184879324A5008333211 @default.
- W3184879324 hasAuthorship W3184879324A5024231734 @default.
- W3184879324 hasAuthorship W3184879324A5060585538 @default.
- W3184879324 hasAuthorship W3184879324A5072311034 @default.
- W3184879324 hasBestOaLocation W31848793241 @default.
- W3184879324 hasConcept C100544194 @default.
- W3184879324 hasConcept C179998833 @default.
- W3184879324 hasConcept C185592680 @default.
- W3184879324 hasConcept C2776673659 @default.
- W3184879324 hasConcept C2776966407 @default.
- W3184879324 hasConcept C2776996202 @default.
- W3184879324 hasConcept C2778641530 @default.
- W3184879324 hasConcept C2779001512 @default.
- W3184879324 hasConcept C2779707754 @default.
- W3184879324 hasConcept C2779858419 @default.
- W3184879324 hasConcept C2780206646 @default.
- W3184879324 hasConcept C2780305828 @default.
- W3184879324 hasConcept C2780782300 @default.
- W3184879324 hasConcept C31903555 @default.
- W3184879324 hasConcept C43617362 @default.
- W3184879324 hasConcept C55493867 @default.
- W3184879324 hasConcept C86803240 @default.
- W3184879324 hasConcept C89423630 @default.
- W3184879324 hasConceptScore W3184879324C100544194 @default.
- W3184879324 hasConceptScore W3184879324C179998833 @default.
- W3184879324 hasConceptScore W3184879324C185592680 @default.
- W3184879324 hasConceptScore W3184879324C2776673659 @default.
- W3184879324 hasConceptScore W3184879324C2776966407 @default.
- W3184879324 hasConceptScore W3184879324C2776996202 @default.
- W3184879324 hasConceptScore W3184879324C2778641530 @default.
- W3184879324 hasConceptScore W3184879324C2779001512 @default.
- W3184879324 hasConceptScore W3184879324C2779707754 @default.
- W3184879324 hasConceptScore W3184879324C2779858419 @default.
- W3184879324 hasConceptScore W3184879324C2780206646 @default.
- W3184879324 hasConceptScore W3184879324C2780305828 @default.
- W3184879324 hasConceptScore W3184879324C2780782300 @default.
- W3184879324 hasConceptScore W3184879324C31903555 @default.
- W3184879324 hasConceptScore W3184879324C43617362 @default.
- W3184879324 hasConceptScore W3184879324C55493867 @default.
- W3184879324 hasConceptScore W3184879324C86803240 @default.
- W3184879324 hasConceptScore W3184879324C89423630 @default.
- W3184879324 hasIssue "3" @default.
- W3184879324 hasLocation W31848793241 @default.
- W3184879324 hasLocation W31848793242 @default.
- W3184879324 hasOpenAccess W3184879324 @default.
- W3184879324 hasPrimaryLocation W31848793241 @default.
- W3184879324 hasRelatedWork W2011632697 @default.
- W3184879324 hasRelatedWork W2025588771 @default.
- W3184879324 hasRelatedWork W2054742562 @default.
- W3184879324 hasRelatedWork W2073239778 @default.
- W3184879324 hasRelatedWork W2101835279 @default.
- W3184879324 hasRelatedWork W2621485457 @default.
- W3184879324 hasRelatedWork W2913261744 @default.
- W3184879324 hasRelatedWork W3115498783 @default.
- W3184879324 hasRelatedWork W4323671120 @default.
- W3184879324 hasRelatedWork W2522390886 @default.
- W3184879324 hasVolume "98" @default.
- W3184879324 isParatext "false" @default.
- W3184879324 isRetracted "false" @default.
- W3184879324 magId "3184879324" @default.
- W3184879324 workType "article" @default.