Matches in SemOpenAlex for { <https://semopenalex.org/work/W3185231875> ?p ?o ?g. }
- W3185231875 endingPage "102" @default.
- W3185231875 startingPage "94" @default.
- W3185231875 abstract "Previously, we showed that tear fluid protects corneal epithelial cells against Pseudomonas aeruginosa without suppressing bacterial viability. Here, we studied how tear fluid affects bacterial gene expression.RNA-sequencing was used to study the P. aeruginosa transcriptome after tear fluid exposure (5 h, 37 oC). Outcomes were further investigated by biochemical and physiological perturbations to tear fluid and tear-like fluid (TLF) and assessment of bacterial viability following tear/TLF pretreatment and antibiotic exposure.Tear fluid deregulated ~180 P. aeruginosa genes ≥8 fold versus PBS including downregulating lasI, rhlI, qscR (quorum sensing/virulence), oprH, phoP, phoQ (antimicrobial resistance) and arnBCADTEF (polymyxin B resistance). Upregulated genes included algF (biofilm formation) and hemO (iron acquisition). qPCR confirmed tear down-regulation of oprH, phoP and phoQ. Tear fluid pre-treatment increased P. aeruginosa resistance to meropenem ~5-fold (4 μg/ml), but enhanced polymyxin B susceptibility ~180-fold (1 μg/ml), the latter activity reduced by dilution in PBS. Media containing a subset of tear components (TLF) also sensitized bacteria to polymyxin B, but only ~22.5-fold, correlating with TLF/tear fluid Ca2+ and Mg2+ concentrations. Accordingly, phoQ mutants were not sensitized by TLF or tear fluid. Superior activity of tear fluid versus TLF against wild-type P. aeruginosa was heat resistant but proteinase K sensitive.P. aeruginosa responds to human tear fluid by upregulating genes associated with bacterial survival and adaptation. Meanwhile, tear fluid down-regulates multiple virulence-associated genes. Tears also utilize divalent cations and heat resistant/proteinase K sensitive component(s) to enhance P. aeruginosa sensitivity to polymyxin B." @default.
- W3185231875 created "2021-08-02" @default.
- W3185231875 creator A5017875495 @default.
- W3185231875 creator A5019391892 @default.
- W3185231875 creator A5026172797 @default.
- W3185231875 creator A5027105364 @default.
- W3185231875 creator A5043170402 @default.
- W3185231875 creator A5069449251 @default.
- W3185231875 creator A5077884151 @default.
- W3185231875 creator A5082957165 @default.
- W3185231875 date "2021-10-01" @default.
- W3185231875 modified "2023-10-15" @default.
- W3185231875 title "Human tear fluid modulates the Pseudomonas aeruginosa transcriptome to alter antibiotic susceptibility" @default.
- W3185231875 cites W1203420015 @default.
- W3185231875 cites W1495321447 @default.
- W3185231875 cites W1512191088 @default.
- W3185231875 cites W1568314805 @default.
- W3185231875 cites W1717865497 @default.
- W3185231875 cites W1970294462 @default.
- W3185231875 cites W1987029769 @default.
- W3185231875 cites W2018961466 @default.
- W3185231875 cites W2039487210 @default.
- W3185231875 cites W2044584162 @default.
- W3185231875 cites W2056073249 @default.
- W3185231875 cites W2060095084 @default.
- W3185231875 cites W2060967918 @default.
- W3185231875 cites W2067985468 @default.
- W3185231875 cites W2080254623 @default.
- W3185231875 cites W2081960029 @default.
- W3185231875 cites W2084277214 @default.
- W3185231875 cites W2093123337 @default.
- W3185231875 cites W2094459550 @default.
- W3185231875 cites W2101664434 @default.
- W3185231875 cites W2105696197 @default.
- W3185231875 cites W2112028148 @default.
- W3185231875 cites W2122722016 @default.
- W3185231875 cites W2130680119 @default.
- W3185231875 cites W2137081432 @default.
- W3185231875 cites W2137965864 @default.
- W3185231875 cites W2141368745 @default.
- W3185231875 cites W2142135721 @default.
- W3185231875 cites W2143635657 @default.
- W3185231875 cites W2144659518 @default.
- W3185231875 cites W2156417794 @default.
- W3185231875 cites W2166813768 @default.
- W3185231875 cites W2169605967 @default.
- W3185231875 cites W2259632819 @default.
- W3185231875 cites W2271297700 @default.
- W3185231875 cites W2342937250 @default.
- W3185231875 cites W2518579334 @default.
- W3185231875 cites W2522285440 @default.
- W3185231875 cites W2569300957 @default.
- W3185231875 cites W2614009534 @default.
- W3185231875 cites W2781117671 @default.
- W3185231875 cites W2885238044 @default.
- W3185231875 cites W2908658955 @default.
- W3185231875 cites W2919783273 @default.
- W3185231875 cites W2927873716 @default.
- W3185231875 cites W2945701292 @default.
- W3185231875 cites W2950097228 @default.
- W3185231875 cites W2972493854 @default.
- W3185231875 cites W2981934833 @default.
- W3185231875 cites W2990045209 @default.
- W3185231875 cites W2991230987 @default.
- W3185231875 cites W2993225104 @default.
- W3185231875 cites W3029218987 @default.
- W3185231875 doi "https://doi.org/10.1016/j.jtos.2021.07.004" @default.
- W3185231875 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34332149" @default.
- W3185231875 hasPublicationYear "2021" @default.
- W3185231875 type Work @default.
- W3185231875 sameAs 3185231875 @default.
- W3185231875 citedByCount "1" @default.
- W3185231875 countsByYear W31852318752022 @default.
- W3185231875 crossrefType "journal-article" @default.
- W3185231875 hasAuthorship W3185231875A5017875495 @default.
- W3185231875 hasAuthorship W3185231875A5019391892 @default.
- W3185231875 hasAuthorship W3185231875A5026172797 @default.
- W3185231875 hasAuthorship W3185231875A5027105364 @default.
- W3185231875 hasAuthorship W3185231875A5043170402 @default.
- W3185231875 hasAuthorship W3185231875A5069449251 @default.
- W3185231875 hasAuthorship W3185231875A5077884151 @default.
- W3185231875 hasAuthorship W3185231875A5082957165 @default.
- W3185231875 hasConcept C104317684 @default.
- W3185231875 hasConcept C135763542 @default.
- W3185231875 hasConcept C185592680 @default.
- W3185231875 hasConcept C2777637488 @default.
- W3185231875 hasConcept C2780026444 @default.
- W3185231875 hasConcept C2780675486 @default.
- W3185231875 hasConcept C4937899 @default.
- W3185231875 hasConcept C501593827 @default.
- W3185231875 hasConcept C523546767 @default.
- W3185231875 hasConcept C540938839 @default.
- W3185231875 hasConcept C54355233 @default.
- W3185231875 hasConcept C55493867 @default.
- W3185231875 hasConcept C58123911 @default.
- W3185231875 hasConcept C60987743 @default.
- W3185231875 hasConcept C77434933 @default.
- W3185231875 hasConcept C86803240 @default.