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- W3185771279 abstract "The gene coding for the triacylglycerol lipase (E.C. 3.1.1.3) of Pseudomonas fragi IFO-12049 was amplified by polymerase chain reaction (PCR). The PCR was performed with two primers carrying EcoRi and HindIIi sites, respectively and chromosomal DNA of P. fragi was used as a template. The PCR product of 1050 base pairs (bp) holding the open-reading frame of lipase gene was ligated with EcoRI linker and inserted into EcoRI site of the pGEX-2T as expression vector. The fused gene was transformed into E. coli MC1061 and amplified. The pGEX-2T harboring lipase gene was isolated and named pGL19. E. coli MC1061 carrying pGL19 was induced by isopropyl-β-D-thiogalactoside (IPTG) and the lipase was highly expressed in E. coli as a fusion protein with glutathione-S-transferase (GST) and exhibited strong lipase activity on the plate containing tributyrin." @default.
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- W3185771279 date "1994-01-01" @default.
- W3185771279 modified "2023-09-23" @default.
- W3185771279 title "Original Articles ; Cloning and Expression of lipase from Pseudomonas fragi as fusion Protesin in Escherichia coli" @default.
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