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- W3185909580 endingPage "100005" @default.
- W3185909580 startingPage "100005" @default.
- W3185909580 abstract "Circadian rhythms in mammals are coordinated by the central clock in the brain, located in the suprachiasmatic nucleus (SCN). Multiple molecular and cellular signals display a circadian variation within SCN neurons, including intracellular Ca 2+ , but the mechanisms are not definitively established. SCN cytosolic Ca 2+ levels exhibit a peak during the day, when both action potential firing and Ca 2+ channel activity are increased, and are decreased at night, correlating with a reduction in firing rate. In this study, we employ a single-color fluorescence anisotropy reporter (FLARE), Venus FLARE-Cameleon, and polarization inverted selective-plane illumination microscopy to measure rhythmic changes in cytosolic Ca 2+ in SCN neurons. Using this technique, the Ca 2+ channel subtypes contributing to intracellular Ca 2+ at the peak and trough of the circadian cycle were assessed using a pharmacological approach with Ca 2+ channel inhibitors. Peak (218 ± 16 nM) and trough (172 ± 13 nM) Ca 2+ levels were quantified, indicating a 1.3-fold circadian variance in Ca 2+ concentration. Inhibition of ryanodine-receptor-mediated Ca 2+ release produced a larger relative decrease in cytosolic Ca 2+ at both time points compared to voltage-gated Ca 2+ channels. These results support the hypothesis that circadian Ca 2+ rhythms in SCN neurons are predominantly driven by intracellular Ca 2+ channels, although not exclusively so. The study provides a foundation for future experiments to probe Ca 2+ signaling in a dynamic biological context using FLAREs." @default.
- W3185909580 created "2021-08-02" @default.
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- W3185909580 creator A5069368810 @default.
- W3185909580 creator A5071118059 @default.
- W3185909580 creator A5090679062 @default.
- W3185909580 date "2021-09-01" @default.
- W3185909580 modified "2023-10-15" @default.
- W3185909580 title "Comparative Ca2+ channel contributions to intracellular Ca2+ levels in the circadian clock" @default.
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- W3185909580 doi "https://doi.org/10.1016/j.bpr.2021.100005" @default.
- W3185909580 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35330949" @default.