FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3186102018> ?p ?o ?g. }

• W3186102018 endingPage "141" @default.
• W3186102018 startingPage "128" @default.
• W3186102018 abstract "<h2>Abstract</h2> microRNA-378a (miR-378a) is one of the most highly expressed microRNAs in the heart. However, its role in the human cardiac tissue has not been fully understood. It was observed that miR-378a protects cardiomyocytes from hypertrophic growth by regulation of IGF1R and the expression of downstream kinases. Increased levels of miR-378a were reported in the serum of Duchenne muscular dystrophy (DMD) patients and female carriers of <i>DMD</i> gene-associated mutations with developed cardiomyopathy. In order to shed more light on the role of miR-378a in human cardiomyocytes and its potential involvement in DMD-related cardiomyopathy, we generated two human induced pluripotent stem cell (hiPSC) models; one with deletion of miR-378a and the second one with deletion of <i>DMD</i> exon 50 leading to the DMD phenotype. Our results indicate that lack of miR-378a does not influence the pluripotency of hiPSC and their ability to differentiate into cardiomyocytes (hiPSC-CM). miR-378a-deficient hiPSC-CM exhibited, however, significantly bigger size compared to the isogenic control cells, indicating the role of this miRNA in the hypertrophic growth of human cardiomyocytes. In accordance, the level of NFATc3, phosphoAKT, phosphoERK and ERK was higher in these cells compared to the control counterparts. A similar effect was achieved by silencing miR-378a with antagomirs. Of note, the percentage of cells with nuclear localization of NFATc3 was higher in miR-378a-deficient hiPSC-CM. Analysis of electrophysiological properties and Ca²⁺ oscillations revealed the decrease in the spike slope velocity and lower frequency of calcium spikes in miR-378a-deficient hiPSC-CM. Interestingly, the level of miR-378a increased gradually during cardiac differentiation of hiPSC. Of note, it was low until day 15 in differentiating <i>DMD</i>-deficient hiPSC-CM and then rose to a similar level as in the isogenic control counterparts. In summary, our findings confirmed the utility of hiPSC-based models for deciphering the role of miR-378a in the control and diseased human cardiomyocytes." @default.
• W3186102018 created "2021-08-02" @default.
• W3186102018 creator A5003571231 @default.
• W3186102018 creator A5007658432 @default.
• W3186102018 creator A5024795564 @default.
• W3186102018 creator A5036864480 @default.
• W3186102018 creator A5043259091 @default.
• W3186102018 creator A5046714339 @default.
• W3186102018 creator A5051986223 @default.
• W3186102018 creator A5082864592 @default.
• W3186102018 date "2021-11-01" @default.
• W3186102018 modified "2023-09-27" @default.
• W3186102018 title "Generation of microRNA-378a-deficient hiPSC as a novel tool to study its role in human cardiomyocytes" @default.
• W3186102018 cites W1975190976 @default.
• W3186102018 cites W1977709885 @default.
• W3186102018 cites W2000797813 @default.
• W3186102018 cites W2051830265 @default.
• W3186102018 cites W2055305152 @default.
• W3186102018 cites W2083599207 @default.
• W3186102018 cites W2088926815 @default.
• W3186102018 cites W2103445003 @default.
• W3186102018 cites W2112484130 @default.
• W3186102018 cites W2130606977 @default.
• W3186102018 cites W2134893281 @default.
• W3186102018 cites W2154578855 @default.
• W3186102018 cites W2159453909 @default.
• W3186102018 cites W2170948963 @default.
• W3186102018 cites W2227838822 @default.
• W3186102018 cites W2408515538 @default.
• W3186102018 cites W2469329896 @default.
• W3186102018 cites W2572179268 @default.
• W3186102018 cites W2579970354 @default.
• W3186102018 cites W2754548624 @default.
• W3186102018 cites W2783365495 @default.
• W3186102018 cites W2800692959 @default.
• W3186102018 cites W2906097696 @default.
• W3186102018 cites W2911312069 @default.
• W3186102018 cites W2965768722 @default.
• W3186102018 cites W2970468269 @default.
• W3186102018 cites W2985354932 @default.
• W3186102018 cites W2992317438 @default.
• W3186102018 cites W3024382227 @default.
• W3186102018 cites W3032401524 @default.
• W3186102018 cites W3118063655 @default.
• W3186102018 doi "https://doi.org/10.1016/j.yjmcc.2021.07.007" @default.
• W3186102018 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34329686" @default.
• W3186102018 hasPublicationYear "2021" @default.
• W3186102018 type Work @default.
• W3186102018 sameAs 3186102018 @default.
• W3186102018 citedByCount "6" @default.
• W3186102018 countsByYear W31861020182022 @default.
• W3186102018 countsByYear W31861020182023 @default.
• W3186102018 crossrefType "journal-article" @default.
• W3186102018 hasAuthorship W3186102018A5003571231 @default.
• W3186102018 hasAuthorship W3186102018A5007658432 @default.
• W3186102018 hasAuthorship W3186102018A5024795564 @default.
• W3186102018 hasAuthorship W3186102018A5036864480 @default.
• W3186102018 hasAuthorship W3186102018A5043259091 @default.
• W3186102018 hasAuthorship W3186102018A5046714339 @default.
• W3186102018 hasAuthorship W3186102018A5051986223 @default.
• W3186102018 hasAuthorship W3186102018A5082864592 @default.
• W3186102018 hasConcept C104317684 @default.
• W3186102018 hasConcept C107459253 @default.
• W3186102018 hasConcept C119056186 @default.
• W3186102018 hasConcept C126322002 @default.
• W3186102018 hasConcept C144174609 @default.
• W3186102018 hasConcept C145059251 @default.
• W3186102018 hasConcept C145103041 @default.
• W3186102018 hasConcept C153911025 @default.
• W3186102018 hasConcept C170493617 @default.
• W3186102018 hasConcept C185592680 @default.
• W3186102018 hasConcept C207200792 @default.
• W3186102018 hasConcept C2775960820 @default.
• W3186102018 hasConcept C2778943923 @default.
• W3186102018 hasConcept C54355233 @default.
• W3186102018 hasConcept C71924100 @default.
• W3186102018 hasConcept C86803240 @default.
• W3186102018 hasConcept C95444343 @default.
• W3186102018 hasConceptScore W3186102018C104317684 @default.
• W3186102018 hasConceptScore W3186102018C107459253 @default.
• W3186102018 hasConceptScore W3186102018C119056186 @default.
• W3186102018 hasConceptScore W3186102018C126322002 @default.
• W3186102018 hasConceptScore W3186102018C144174609 @default.
• W3186102018 hasConceptScore W3186102018C145059251 @default.
• W3186102018 hasConceptScore W3186102018C145103041 @default.
• W3186102018 hasConceptScore W3186102018C153911025 @default.
• W3186102018 hasConceptScore W3186102018C170493617 @default.
• W3186102018 hasConceptScore W3186102018C185592680 @default.
• W3186102018 hasConceptScore W3186102018C207200792 @default.
• W3186102018 hasConceptScore W3186102018C2775960820 @default.
• W3186102018 hasConceptScore W3186102018C2778943923 @default.
• W3186102018 hasConceptScore W3186102018C54355233 @default.
• W3186102018 hasConceptScore W3186102018C71924100 @default.
• W3186102018 hasConceptScore W3186102018C86803240 @default.
• W3186102018 hasConceptScore W3186102018C95444343 @default.
• W3186102018 hasLocation W31861020181 @default.
• W3186102018 hasLocation W31861020182 @default.
• W3186102018 hasOpenAccess W3186102018 @default.

• next