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• W3186122621 abstract "Abstract The honey bee is an excellent model system to study behavioral ecology, behavioral genetics, and sociogenomics. Nucleic acid-based analyses enable a broad scope of research in functional genomics, disease diagnostics, mutant screening, and genetic breeding. Multiple levels of analysis lead to a more comprehensive understanding of the causes of phenotypic variation by integrating genomic variation, transcriptomic profiles, and epigenomic information. One limitation, however, is the sample preparation procedures to obtain high quality DNA and RNA simultaneously, particularly from small amounts of material, such as tissues of individual bees. We demonstrate that it is feasible to perform dual extractions of DNA and RNA from a single individual bee and compare the quality and quantity of the extracted nucleic acids using two different types of methods. There was a greater total yield of DNA and RNA from ethanol-based extractions with minimal differences in overall concentration in ng/uL. We describe here the first validated method for dual extraction of DNA and RNA specifically from individual honey bees (Apis mellifera)." @default.
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• W3186122621 date "2021-07-27" @default.
• W3186122621 modified "2023-10-07" @default.
• W3186122621 title "Assessment and Comparison of Two Different Methods to Extract Nucleic Acids From Individual Honey Bees" @default.
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• W3186122621 doi "https://doi.org/10.1093/aesa/saab027" @default.
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