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• W3186146557 endingPage "159005" @default.
• W3186146557 startingPage "159005" @default.
• W3186146557 abstract "Accumulation of excess cholesterol and cholesteryl ester in macrophage ‘foam’ cells within the arterial intima characterises early ‘fatty streak’ atherosclerotic lesions, and is accompanied by epigenetic changes, including altered expression of microRNA sequences which determine of gene and protein expression. This study established that exposure to lipoproteins, including acetylated LDL, induced macrophage expression of microRNA hsa-let-7d-5p, a sequence previously linked with tumour suppression, and repressed expression of one of its target genes, high mobility group AT hook 2 ( HMGA2 ). A let-7d-5p mimic repressed expression of HMGA2 (18%; p < 0.05) while a marked increase (2.9-fold; p < 0.05) in expression of HMGA2 was noted in the presence of let-7d-5p inhibitor. Under these conditions, let-7d-5p mimic significantly (p < 0.05) decreased total (10%), free (8%) and cholesteryl ester (21%) mass, while the inhibitor significantly (p < 0.05) increased total (29%) and free cholesterol (29%) mass, compared with the relevant controls. Let-7d-5p inhibition significantly (p < 0.05) increased endogenous biosynthesis of cholesterol (38%) and cholesteryl ester (39%) pools in macrophage ‘foam’ cells, without altering the cholesterol efflux pathway, or esterification of exogenous radiolabelled oleate. Let-7d-5p inhibition in sterol-loaded cells increased the level of HMGA2 protein (32%; p < 0.05), while SiRNA knockdown of this protein (29%; p < 0.05) resulted in a (21%, p < 0.05) reduction in free cholesterol mass. Thus, induction of let-7d-5p, and repression of its target HMGA2, in macrophages is a protective response to the challenge of increased cholesterol influx into these cells; dysregulation of this response may contribute to atherosclerosis and other disorders such as cancer. • Lipoproteins induce expression of hsa-let-7d-5p, a sequence linked with tumour suppression and cell differentiation. • Let-7d-5p targets high mobility group AT-hook 2 (HMGA2), a non-histone architectural transcription factor. • Inhibition of let-7d-5p increases HMGA2 expression in macrophages exposed to a cholesterol load. • Knockdown of HMGA2 reduces mass of free cholesterol in macrophages. • Inhibition of let-7d-5p increases total cholesterol mass, and endogenous biosynthesis of cholesterol and cholesteryl ester. • Induction of let-7d-5p in macrophages provides protection against increased cholesterol influx into these cells." @default.
• W3186146557 created "2021-08-02" @default.
• W3186146557 creator A5012374119 @default.
• W3186146557 creator A5019474867 @default.
• W3186146557 creator A5023011000 @default.
• W3186146557 creator A5064239988 @default.
• W3186146557 date "2021-11-01" @default.
• W3186146557 modified "2023-09-26" @default.
• W3186146557 title "Induction of microRNA hsa-let-7d-5p, and repression of HMGA2, contribute protection against lipid accumulation in macrophage ‘foam’ cells" @default.
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