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- W3186156328 abstract "The number of described species of the oomycete genus Phytophthora is growing rapidly, highlighting the need for low-cost, rapid tools for species identification. Here, a collection of 24 Phytophthora species (42 samples) from natural as well as anthropogenic habitats were genetically identified using the internal transcribed spacer (ITS) and cytochrome c oxidase subunit I (COI) regions. Because genetic identification is time consuming, we have created a complementary method based on by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). Both methods were compared and hypothesis that the MALDI-TOF MS method can be a fast and reliable method for the identification of oomycetes was confirmed. Over 3500 mass spectra were acquired, manually reviewed for quality control, and consolidated into a single reference library using the Bruker MALDI Biotyper platform. Finally, a database containing 144 main spectra (MSPs) was created and published in repository. The method presented in this study will facilitate the use of MALDI-TOF MS as a complement to existing approaches for fast, reliable identification of Phytophthora isolates." @default.
- W3186156328 created "2021-08-02" @default.
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- W3186156328 date "2021-07-19" @default.
- W3186156328 modified "2023-09-24" @default.
- W3186156328 title "MALDI-TOF MS as a method for rapid identification of <i><i>Phytophthora</i></i> de Bary, 1876" @default.
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- W3186156328 doi "https://doi.org/10.7717/peerj.11662" @default.
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