FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3186582930> ?p ?o ?g. }

• W3186582930 endingPage "84" @default.
• W3186582930 startingPage "76" @default.
• W3186582930 abstract "Fludioxnil is extensively used as a fungicide in agricultural application, but its possible impact on embryonic development is not yet well understood. In this study, the potential effect of fludioxonil on cardiac differentiation was evaluated in mouse embryonic stem cells (mESCs). The water-soluble tetrazolium (WST) and colony formation assays were conducted to confirm the effect of fludioxonil on proliferation of mESCs. The effect of fludioxonil on the ability of mESCs to form mouse embryoid bodies (mEBs) was determined by the hanging drop assay, whereas the ability of cardiomyocyte differentiation in the early stage was evaluated by determining the beating ratio (ratio of the number of contracting cells to the number of attached EBs) of cardiomyocytes. The viability of mESCs was significantly decreased (less than 50 %) at 10-5 M fludioxonil. Results of the colony formation assay revealed suppressed colony formation at 10-5 M fludioxonil (about 50 % at 5 days). Furthermore, the expressions of cell-cycle related proteins, i.e., cyclin D1, cyclin E, p21 and p27, were altered and trending towards inhibiting cell growth. Exposure to fludioxonil also resulted in reduced size of the mEB and induced increasing expression levels of the pluripotency markers Oct4, Sox2 and Nanog. Development of the beating ratio in the process of differentiation to cardiomyocytes derived from mESCs was completely inhibited after exposure to 10-5 M fludioxonil during the early stage of differentiation (day 5), whereas the beating ratio gradually increased after 5-day treatment. Simultaneously, expressions of the cardiomyocyte-related proteins, Gata4, Hand1 and cTnI, were inhibited after exposure to 10-5 M fludioxonil. Taken together, these results imply that fludioxonil may impact on the developmental process of mESCs, particularly the cardiac lineage." @default.
• W3186582930 created "2021-08-02" @default.
• W3186582930 creator A5000433598 @default.
• W3186582930 creator A5004961134 @default.
• W3186582930 creator A5063721180 @default.
• W3186582930 creator A5070989428 @default.
• W3186582930 creator A5081165990 @default.
• W3186582930 date "2021-09-01" @default.
• W3186582930 modified "2023-09-27" @default.
• W3186582930 title "Effect of the phenylpyrrole fungicide fludioxonil on cell proliferation and cardiac differentiation in mouse embryonic stem cells" @default.
• W3186582930 cites W145559274 @default.
• W3186582930 cites W1535291574 @default.
• W3186582930 cites W1956857062 @default.
• W3186582930 cites W1965428318 @default.
• W3186582930 cites W1972075005 @default.
• W3186582930 cites W1972305847 @default.
• W3186582930 cites W1991199800 @default.
• W3186582930 cites W1993477468 @default.
• W3186582930 cites W2020386821 @default.
• W3186582930 cites W2021505095 @default.
• W3186582930 cites W2029227820 @default.
• W3186582930 cites W2038484136 @default.
• W3186582930 cites W2041450701 @default.
• W3186582930 cites W2062220093 @default.
• W3186582930 cites W2062310827 @default.
• W3186582930 cites W2081928855 @default.
• W3186582930 cites W2083558557 @default.
• W3186582930 cites W2086943348 @default.
• W3186582930 cites W2098412103 @default.
• W3186582930 cites W2098983125 @default.
• W3186582930 cites W2108344711 @default.
• W3186582930 cites W2115798328 @default.
• W3186582930 cites W2132834292 @default.
• W3186582930 cites W2134066558 @default.
• W3186582930 cites W2136071912 @default.
• W3186582930 cites W2148146693 @default.
• W3186582930 cites W2158809385 @default.
• W3186582930 cites W2509057624 @default.
• W3186582930 cites W2515736350 @default.
• W3186582930 cites W2544689575 @default.
• W3186582930 cites W2606849963 @default.
• W3186582930 cites W2728366731 @default.
• W3186582930 cites W2751310957 @default.
• W3186582930 cites W2901069320 @default.
• W3186582930 cites W2968547836 @default.
• W3186582930 cites W2972431724 @default.
• W3186582930 cites W2973295346 @default.
• W3186582930 cites W4211194339 @default.
• W3186582930 cites W4251197476 @default.
• W3186582930 cites W84590241 @default.
• W3186582930 doi "https://doi.org/10.1016/j.reprotox.2021.07.006" @default.
• W3186582930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34280493" @default.
• W3186582930 hasPublicationYear "2021" @default.
• W3186582930 type Work @default.
• W3186582930 sameAs 3186582930 @default.
• W3186582930 citedByCount "2" @default.
• W3186582930 countsByYear W31865829302022 @default.
• W3186582930 countsByYear W31865829302023 @default.
• W3186582930 crossrefType "journal-article" @default.
• W3186582930 hasAuthorship W3186582930A5000433598 @default.
• W3186582930 hasAuthorship W3186582930A5004961134 @default.
• W3186582930 hasAuthorship W3186582930A5063721180 @default.
• W3186582930 hasAuthorship W3186582930A5070989428 @default.
• W3186582930 hasAuthorship W3186582930A5081165990 @default.
• W3186582930 hasConcept C104317684 @default.
• W3186582930 hasConcept C107459253 @default.
• W3186582930 hasConcept C143948831 @default.
• W3186582930 hasConcept C145103041 @default.
• W3186582930 hasConcept C148738053 @default.
• W3186582930 hasConcept C1491633281 @default.
• W3186582930 hasConcept C153911025 @default.
• W3186582930 hasConcept C185592680 @default.
• W3186582930 hasConcept C207701140 @default.
• W3186582930 hasConcept C2777588181 @default.
• W3186582930 hasConcept C2777933648 @default.
• W3186582930 hasConcept C28328180 @default.
• W3186582930 hasConcept C29537977 @default.
• W3186582930 hasConcept C55493867 @default.
• W3186582930 hasConcept C59822182 @default.
• W3186582930 hasConcept C74600772 @default.
• W3186582930 hasConcept C86803240 @default.
• W3186582930 hasConcept C95444343 @default.
• W3186582930 hasConceptScore W3186582930C104317684 @default.
• W3186582930 hasConceptScore W3186582930C107459253 @default.
• W3186582930 hasConceptScore W3186582930C143948831 @default.
• W3186582930 hasConceptScore W3186582930C145103041 @default.
• W3186582930 hasConceptScore W3186582930C148738053 @default.
• W3186582930 hasConceptScore W3186582930C1491633281 @default.
• W3186582930 hasConceptScore W3186582930C153911025 @default.
• W3186582930 hasConceptScore W3186582930C185592680 @default.
• W3186582930 hasConceptScore W3186582930C207701140 @default.
• W3186582930 hasConceptScore W3186582930C2777588181 @default.
• W3186582930 hasConceptScore W3186582930C2777933648 @default.
• W3186582930 hasConceptScore W3186582930C28328180 @default.
• W3186582930 hasConceptScore W3186582930C29537977 @default.
• W3186582930 hasConceptScore W3186582930C55493867 @default.
• W3186582930 hasConceptScore W3186582930C59822182 @default.
• W3186582930 hasConceptScore W3186582930C74600772 @default.

• next