Matches in SemOpenAlex for { <https://semopenalex.org/work/W3187016744> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3187016744 endingPage "2442" @default.
- W3187016744 startingPage "2435" @default.
- W3187016744 abstract "The stability of virus-like particles (VLPs) is currently the main factor affecting the quality of foot-and-mouth disease VLPs vaccines. In order to further improve the quality of the VLPs vaccine of foot-and-mouth disease (FMD), three amino acid modification sites were designed and screened through kinetic analysis software, based on the three-dimensional structure of FMDV. The three mutant recombinant plasmids were successfully prepared by the point mutation kit, transformed into Escherichia coli strain BL21 and expressed in vitro. After purification by Ni ion chromatography column, SDS-PAGE proved that the three amino acid mutations did not affect the expression of the target protein. The results of the stability study of three FMD mutant VLPs obtained by in vitro assembly show that the introduction of internal hydrophobic side chain amino acids made the morphology of VLPs more uniform (N4017W), and their stability was significantly improved compared to the other two VLPs. The internal hydrophobic force of the capsid contributes to the formation of VLPs and helps to maintain the stability of the capsid, providing new experimental ideas for improving the quality of VLPs vaccines, and helping to promote the development of VLPs vaccines." @default.
- W3187016744 created "2021-08-02" @default.
- W3187016744 creator A5017261400 @default.
- W3187016744 creator A5019270870 @default.
- W3187016744 creator A5021319104 @default.
- W3187016744 creator A5036907897 @default.
- W3187016744 creator A5082754243 @default.
- W3187016744 creator A5085968117 @default.
- W3187016744 date "2021-07-25" @default.
- W3187016744 modified "2023-09-27" @default.
- W3187016744 title "[Effect of amino acid site modification on stability of foot-and-mouth disease virus-like particles]." @default.
- W3187016744 doi "https://doi.org/10.13345/j.cjb.200551" @default.
- W3187016744 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34327908" @default.
- W3187016744 hasPublicationYear "2021" @default.
- W3187016744 type Work @default.
- W3187016744 sameAs 3187016744 @default.
- W3187016744 citedByCount "1" @default.
- W3187016744 countsByYear W31870167442022 @default.
- W3187016744 crossrefType "journal-article" @default.
- W3187016744 hasAuthorship W3187016744A5017261400 @default.
- W3187016744 hasAuthorship W3187016744A5019270870 @default.
- W3187016744 hasAuthorship W3187016744A5021319104 @default.
- W3187016744 hasAuthorship W3187016744A5036907897 @default.
- W3187016744 hasAuthorship W3187016744A5082754243 @default.
- W3187016744 hasAuthorship W3187016744A5085968117 @default.
- W3187016744 hasConcept C104317684 @default.
- W3187016744 hasConcept C143065580 @default.
- W3187016744 hasConcept C159047783 @default.
- W3187016744 hasConcept C185592680 @default.
- W3187016744 hasConcept C202751555 @default.
- W3187016744 hasConcept C202878990 @default.
- W3187016744 hasConcept C22744801 @default.
- W3187016744 hasConcept C2522874641 @default.
- W3187016744 hasConcept C2776151397 @default.
- W3187016744 hasConcept C40767141 @default.
- W3187016744 hasConcept C515207424 @default.
- W3187016744 hasConcept C547475151 @default.
- W3187016744 hasConcept C552990157 @default.
- W3187016744 hasConcept C55493867 @default.
- W3187016744 hasConcept C86803240 @default.
- W3187016744 hasConceptScore W3187016744C104317684 @default.
- W3187016744 hasConceptScore W3187016744C143065580 @default.
- W3187016744 hasConceptScore W3187016744C159047783 @default.
- W3187016744 hasConceptScore W3187016744C185592680 @default.
- W3187016744 hasConceptScore W3187016744C202751555 @default.
- W3187016744 hasConceptScore W3187016744C202878990 @default.
- W3187016744 hasConceptScore W3187016744C22744801 @default.
- W3187016744 hasConceptScore W3187016744C2522874641 @default.
- W3187016744 hasConceptScore W3187016744C2776151397 @default.
- W3187016744 hasConceptScore W3187016744C40767141 @default.
- W3187016744 hasConceptScore W3187016744C515207424 @default.
- W3187016744 hasConceptScore W3187016744C547475151 @default.
- W3187016744 hasConceptScore W3187016744C552990157 @default.
- W3187016744 hasConceptScore W3187016744C55493867 @default.
- W3187016744 hasConceptScore W3187016744C86803240 @default.
- W3187016744 hasIssue "7" @default.
- W3187016744 hasLocation W31870167441 @default.
- W3187016744 hasOpenAccess W3187016744 @default.
- W3187016744 hasPrimaryLocation W31870167441 @default.
- W3187016744 hasRelatedWork W1975602674 @default.
- W3187016744 hasRelatedWork W1980134346 @default.
- W3187016744 hasRelatedWork W1993090726 @default.
- W3187016744 hasRelatedWork W2013366520 @default.
- W3187016744 hasRelatedWork W2048174867 @default.
- W3187016744 hasRelatedWork W2070287377 @default.
- W3187016744 hasRelatedWork W2081983857 @default.
- W3187016744 hasRelatedWork W2082221651 @default.
- W3187016744 hasRelatedWork W2106517331 @default.
- W3187016744 hasRelatedWork W2151908837 @default.
- W3187016744 hasRelatedWork W2155222327 @default.
- W3187016744 hasRelatedWork W2385762294 @default.
- W3187016744 hasRelatedWork W3101412428 @default.
- W3187016744 hasRelatedWork W3132641501 @default.
- W3187016744 hasRelatedWork W3198059857 @default.
- W3187016744 hasRelatedWork W3202647507 @default.
- W3187016744 hasRelatedWork W2278580013 @default.
- W3187016744 hasRelatedWork W2757354239 @default.
- W3187016744 hasRelatedWork W2838566029 @default.
- W3187016744 hasRelatedWork W2988306196 @default.
- W3187016744 hasVolume "37" @default.
- W3187016744 isParatext "false" @default.
- W3187016744 isRetracted "false" @default.
- W3187016744 magId "3187016744" @default.
- W3187016744 workType "article" @default.