Matches in SemOpenAlex for { <https://semopenalex.org/work/W3187088979> ?p ?o ?g. }
- W3187088979 endingPage "8555" @default.
- W3187088979 startingPage "8535" @default.
- W3187088979 abstract "Abstract Gene deletion and gene expression alteration can lead to growth defects that are amplified or reduced when a second mutation is present in the same cells. We performed 154 genetic interaction mapping (GIM) screens with query mutants related with RNA metabolism and estimated the growth rates of about 700 000 double mutant Saccharomyces cerevisiae strains. The tested targets included the gene deletion collection and 900 strains in which essential genes were affected by mRNA destabilization (DAmP). To analyze the results, we developed RECAP, a strategy that validates genetic interaction profiles by comparison with gene co-citation frequency, and identified links between 1471 genes and 117 biological processes. In addition to these large-scale results, we validated both enhancement and suppression of slow growth measured for specific RNA-related pathways. Thus, negative genetic interactions identified a role for the OCA inositol polyphosphate hydrolase complex in mRNA translation initiation. By analysis of suppressors, we found that Puf4, a Pumilio family RNA binding protein, inhibits ribosomal protein Rpl9 function, by acting on a conserved UGUAcauUA motif located downstream the stop codon of the RPL9B mRNA. Altogether, the results and their analysis should represent a useful resource for discovery of gene function in yeast." @default.
- W3187088979 created "2021-08-16" @default.
- W3187088979 creator A5042317792 @default.
- W3187088979 creator A5070536515 @default.
- W3187088979 creator A5082044649 @default.
- W3187088979 creator A5084337876 @default.
- W3187088979 creator A5088118853 @default.
- W3187088979 date "2021-08-06" @default.
- W3187088979 modified "2023-10-14" @default.
- W3187088979 title "Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast" @default.
- W3187088979 cites W1860702156 @default.
- W3187088979 cites W1963760518 @default.
- W3187088979 cites W1966072209 @default.
- W3187088979 cites W1967042968 @default.
- W3187088979 cites W1970250905 @default.
- W3187088979 cites W1974350858 @default.
- W3187088979 cites W1985432682 @default.
- W3187088979 cites W1989768108 @default.
- W3187088979 cites W1990873429 @default.
- W3187088979 cites W1994267372 @default.
- W3187088979 cites W1994624068 @default.
- W3187088979 cites W1996131817 @default.
- W3187088979 cites W1997168691 @default.
- W3187088979 cites W2000149489 @default.
- W3187088979 cites W2013242034 @default.
- W3187088979 cites W2022523272 @default.
- W3187088979 cites W2022589976 @default.
- W3187088979 cites W2024931756 @default.
- W3187088979 cites W2025309924 @default.
- W3187088979 cites W2027145418 @default.
- W3187088979 cites W2029863651 @default.
- W3187088979 cites W2036314702 @default.
- W3187088979 cites W2042688172 @default.
- W3187088979 cites W2047516322 @default.
- W3187088979 cites W2049402769 @default.
- W3187088979 cites W2053184946 @default.
- W3187088979 cites W2058262378 @default.
- W3187088979 cites W2060718885 @default.
- W3187088979 cites W2066825851 @default.
- W3187088979 cites W2082610820 @default.
- W3187088979 cites W2087576311 @default.
- W3187088979 cites W2095722471 @default.
- W3187088979 cites W2101888232 @default.
- W3187088979 cites W2102221598 @default.
- W3187088979 cites W2103017472 @default.
- W3187088979 cites W2104872953 @default.
- W3187088979 cites W2107067224 @default.
- W3187088979 cites W2108098152 @default.
- W3187088979 cites W2109535551 @default.
- W3187088979 cites W2110453129 @default.
- W3187088979 cites W2119635233 @default.
- W3187088979 cites W2120959840 @default.
- W3187088979 cites W2123237534 @default.
- W3187088979 cites W2125762953 @default.
- W3187088979 cites W2130410032 @default.
- W3187088979 cites W2131681506 @default.
- W3187088979 cites W2132770871 @default.
- W3187088979 cites W2133102806 @default.
- W3187088979 cites W2134224359 @default.
- W3187088979 cites W2138470019 @default.
- W3187088979 cites W2139238510 @default.
- W3187088979 cites W2142437744 @default.
- W3187088979 cites W2143341850 @default.
- W3187088979 cites W2143793902 @default.
- W3187088979 cites W2143962926 @default.
- W3187088979 cites W2145068784 @default.
- W3187088979 cites W2145172772 @default.
- W3187088979 cites W2145866983 @default.
- W3187088979 cites W2146264532 @default.
- W3187088979 cites W2147092264 @default.
- W3187088979 cites W2152311253 @default.
- W3187088979 cites W2153134824 @default.
- W3187088979 cites W2157980476 @default.
- W3187088979 cites W2159460045 @default.
- W3187088979 cites W2159675211 @default.
- W3187088979 cites W2160373402 @default.
- W3187088979 cites W2171038628 @default.
- W3187088979 cites W2176178891 @default.
- W3187088979 cites W2177016155 @default.
- W3187088979 cites W2180876606 @default.
- W3187088979 cites W2277781307 @default.
- W3187088979 cites W2291775309 @default.
- W3187088979 cites W2427010553 @default.
- W3187088979 cites W2522417827 @default.
- W3187088979 cites W2537623931 @default.
- W3187088979 cites W2546667113 @default.
- W3187088979 cites W2588633819 @default.
- W3187088979 cites W2611963968 @default.
- W3187088979 cites W2736001740 @default.
- W3187088979 cites W2774019174 @default.
- W3187088979 cites W2789889445 @default.
- W3187088979 cites W2810907434 @default.
- W3187088979 cites W2892403696 @default.
- W3187088979 cites W2894282821 @default.
- W3187088979 cites W2916171022 @default.
- W3187088979 cites W2917858269 @default.
- W3187088979 cites W2922105014 @default.
- W3187088979 cites W2924119339 @default.