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- W3187636553 abstract "We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases." @default.
- W3187636553 created "2021-08-16" @default.
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- W3187636553 date "2021-08-05" @default.
- W3187636553 modified "2023-10-10" @default.
- W3187636553 title "MALDI-TOF MS identification of <i>Prototheca</i> algae associated with bovine mastitis" @default.
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- W3187636553 doi "https://doi.org/10.1177/10406387211036459" @default.
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