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- W3187988762 endingPage "7087" @default.
- W3187988762 startingPage "7087" @default.
- W3187988762 abstract "The development of sensitive and selective assays for protein biomarkers and other biological analytes is important for advancing the fields of clinical diagnostics and bioanalytical chemistry. The potential advantages of using aptamers in electrochemical sandwich assays are being increasingly recognized. These assays may include an aptamer as both capture and detection agent or a combination of an aptamer with a different partner such as an antibody, a lectin or a nanomaterial. The second binding partner in the sandwich structure is typically conjugated to a redox marker, a catalyst or an enzyme that can be used to generate the signal needed for electrochemical detection. Nanoparticles and other nanostructures can be used as the carriers for multiple molecules of the detection partner and thereby increase the signal. Nanostructured surfaces can be used to increase surface area and improve electron transfer. Sensitive electrochemical methods including impedance, differential and square-wave voltammetry and chronocoulometry have been used for electrochemical signal read-out. Impressive results have been achieved using electrochemical sandwich assays in terms of limit of detection and linear range for a growing range of analytes. The recent progress for this type of assay for proteins and other biomarkers is the subject of this review." @default.
- W3187988762 created "2021-08-16" @default.
- W3187988762 creator A5019463131 @default.
- W3187988762 creator A5056329054 @default.
- W3187988762 date "2021-07-31" @default.
- W3187988762 modified "2023-10-02" @default.
- W3187988762 title "Electrochemical Sandwich Assays for Biomarkers Incorporating Aptamers, Antibodies and Nanomaterials for Detection of Specific Protein Biomarkers" @default.
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