FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3188368846> ?p ?o ?g. }

• W3188368846 abstract "Abstract Study question Do delayed mature oocytes result in similar euploid blastocyst rates as their immediate mature sibling oocytes? Summary answer Once a blastocyst is obtained, delayed mature oocytes have similar euploid rates compared to immediate mature oocytes. What is known already Intracytoplasmic sperm injection (ICSI) of metaphase II oocytes few hours post oocyte retrieval is standard practice in IVF laboratories. Immature metaphase I (MI) and prophase I (GV) oocytes are usually discarded. Immature oocytes may mature overnight, after which ICSI can be performed. Studies demonstrated lower fertilization and blastulation rates for these delayed mature oocytes. However, live births have been reported from blastocysts transferred. The evidence available is not compelling, since most of the studies had either low sample size, no preimplantation genetic testing for aneuploidies (PGT-A), or the outcome was not compared to sibling MII oocytes at time of denudation. Study design, size, duration A single-center retrospective sibling oocyte study was performed between January 2019 and December 2020 at ART Fertility clinics Abu Dhabi, UAE. A total of 345 PGT-A cycles, with at least one delayed mature oocyte inseminated by ICSI, were included: 2506 immediate mature oocytes and 669 delayed mature oocytes. Participants/materials, setting, methods Following controlled ovarian stimulation, MII oocytes at the time of denudation were inseminated by ICSI/IVF (immediate mature). Immature oocytes (MI/GV) were cultured for 16–24 hours in fertilization medium and injected the next day if matured (delayed mature). Trophectoderm biopsy was performed on day 5/6/7 and samples were subjected to Next Generation Sequencing to screen the ploidy state of the blastocyst. Main results and the role of chance The 345 controlled ovarian stimulation cycles resulted in the insemination of 2506 MII oocytes on the day of oocyte retrieval (Day0) and 669 delayed mature oocytes on day 1. Normal fertilization rate was significantly higher in the immediate mature oocytes compared to delayed mature oocytes (68% vs 56%, p &lt; 0.0001). Similarly, the usable blastocyst rate was significantly higher in immediate mature oocytes (59% vs 19%, p &lt; 0.0001). On day 5 of development, a significantly higher-good quality blastocyst formation rate was obtained from immediate mature oocytes (65% vs 27%, p &lt; 0.0001). The rate of good quality blastocyst on the day of biopsy was significantly higher in the immediate mature oocytes group (76% vs 62%, p &lt; 0.015). Fisher’s Exact Test was performed to compare the euploid rate of blastocysts biopsied on day 5/6/7 originating from immediate mature oocytes or sibling delayed mature oocytes. The euploid potential of blastocyst biopsied showed no significant difference between the two groups (p = 0.388). Limitations, reasons for caution The timing of MI/GV oocytes transition to MII stage was not recorded since the incubation was done in a benchtop incubator. Furthermore, the same sperm sample was used to inseminate immediate and delayed mature oocytes, which might contribute to the compromised embryo development due to increased sperm DNA fragmentation. Wider implications of the findings: Insemination of delayed mature oocytes by ICSI, should be considered as a tool to increase patients’ chances of obtaining a euploid embryo. Especially in cases where low yield of euploid embryos is expected. Trial registration number Not applicable" @default.
• W3188368846 created "2021-08-16" @default.
• W3188368846 creator A5019851514 @default.
• W3188368846 creator A5021156785 @default.
• W3188368846 creator A5022118127 @default.
• W3188368846 creator A5040903293 @default.
• W3188368846 creator A5044731754 @default.
• W3188368846 creator A5047037826 @default.
• W3188368846 creator A5048445694 @default.
• W3188368846 creator A5072875016 @default.
• W3188368846 creator A5083631105 @default.
• W3188368846 date "2021-07-01" @default.
• W3188368846 modified "2023-10-16" @default.
• W3188368846 title "P–271 Should intracytoplasmic sperm injection (ICSI) of delayed mature oocytes become a routine practice in the IVF Laboratory?" @default.
• W3188368846 doi "https://doi.org/10.1093/humrep/deab130.270" @default.
• W3188368846 hasPublicationYear "2021" @default.
• W3188368846 type Work @default.
• W3188368846 sameAs 3188368846 @default.
• W3188368846 citedByCount "0" @default.
• W3188368846 crossrefType "journal-article" @default.
• W3188368846 hasAuthorship W3188368846A5019851514 @default.
• W3188368846 hasAuthorship W3188368846A5021156785 @default.
• W3188368846 hasAuthorship W3188368846A5022118127 @default.
• W3188368846 hasAuthorship W3188368846A5040903293 @default.
• W3188368846 hasAuthorship W3188368846A5044731754 @default.
• W3188368846 hasAuthorship W3188368846A5047037826 @default.
• W3188368846 hasAuthorship W3188368846A5048445694 @default.
• W3188368846 hasAuthorship W3188368846A5072875016 @default.
• W3188368846 hasAuthorship W3188368846A5083631105 @default.
• W3188368846 hasConcept C105702510 @default.
• W3188368846 hasConcept C16685009 @default.
• W3188368846 hasConcept C196843134 @default.
• W3188368846 hasConcept C2776690073 @default.
• W3188368846 hasConcept C2778177303 @default.
• W3188368846 hasConcept C2778278907 @default.
• W3188368846 hasConcept C2779234561 @default.
• W3188368846 hasConcept C29456083 @default.
• W3188368846 hasConcept C512716672 @default.
• W3188368846 hasConcept C54355233 @default.
• W3188368846 hasConcept C71924100 @default.
• W3188368846 hasConcept C86803240 @default.
• W3188368846 hasConcept C87073359 @default.
• W3188368846 hasConcept C88972607 @default.
• W3188368846 hasConceptScore W3188368846C105702510 @default.
• W3188368846 hasConceptScore W3188368846C16685009 @default.
• W3188368846 hasConceptScore W3188368846C196843134 @default.
• W3188368846 hasConceptScore W3188368846C2776690073 @default.
• W3188368846 hasConceptScore W3188368846C2778177303 @default.
• W3188368846 hasConceptScore W3188368846C2778278907 @default.
• W3188368846 hasConceptScore W3188368846C2779234561 @default.
• W3188368846 hasConceptScore W3188368846C29456083 @default.
• W3188368846 hasConceptScore W3188368846C512716672 @default.
• W3188368846 hasConceptScore W3188368846C54355233 @default.
• W3188368846 hasConceptScore W3188368846C71924100 @default.
• W3188368846 hasConceptScore W3188368846C86803240 @default.
• W3188368846 hasConceptScore W3188368846C87073359 @default.
• W3188368846 hasConceptScore W3188368846C88972607 @default.
• W3188368846 hasIssue "Supplement_1" @default.
• W3188368846 hasLocation W31883688461 @default.
• W3188368846 hasOpenAccess W3188368846 @default.
• W3188368846 hasPrimaryLocation W31883688461 @default.
• W3188368846 hasRelatedWork W1963685898 @default.
• W3188368846 hasRelatedWork W2026817348 @default.
• W3188368846 hasRelatedWork W2069766861 @default.
• W3188368846 hasRelatedWork W2136549887 @default.
• W3188368846 hasRelatedWork W2330084571 @default.
• W3188368846 hasRelatedWork W2520261096 @default.
• W3188368846 hasRelatedWork W2526891425 @default.
• W3188368846 hasRelatedWork W2587844416 @default.
• W3188368846 hasRelatedWork W2819637198 @default.
• W3188368846 hasRelatedWork W4214729304 @default.
• W3188368846 hasVolume "36" @default.
• W3188368846 isParatext "false" @default.
• W3188368846 isRetracted "false" @default.
• W3188368846 magId "3188368846" @default.
• W3188368846 workType "article" @default.