FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3188373912> ?p ?o ?g. }

• W3188373912 abstract "Conjunctival epithelium forms a barrier between the ocular surface microbial flora and the ocular mucosa. In addition to secreting gel-forming mucins, goblet cells, located in the conjunctival epithelium, help maintain local immune homeostasis by secreting active TGFβ2 and promoting tolerogenic phenotype of dendritic cells in the vicinity. Although dendritic cell subsets, characteristic of mucosal tissues, are found in the conjunctiva, previous studies provided limited information about their location within the tissue. In this study, we examine immunostained conjunctiva explants to determine the location of CD11c-positive dendritic cells in the context of MUC5AC-positive goblet cells. Considering that conjunctival goblet cells are responsive to signaling induced by pathogen recognition receptors, we also assess if their responses to microbial product, flagellin, can contribute to the disruption of ocular mucosal homeostasis that promotes activation of dendritic cells and results in chronic ocular surface inflammation. We find that dendritic cells in the conjunctiva with an increased microbial colonization are located adjacent to goblet cells. While their cell bodies in the stromal layer are immediately below the epithelial layer, several extensions of dendritic cells are projected across the epithelium towards the ocular surface. Such trans-epithelial dendrites are not detectable in healthy ocular mucosa. In response to topically applied flagellin, increased proportion of CD11c-positive cells in the conjunctiva strongly express MHC class II relative to the untreated conjunctiva. This change is accompanied by reduced immunoreactivity to TGFβ-activating Thrombospondin-1 in the conjunctival epithelium. These findings are supported by in vitro observations in primary cultures of goblet cells that respond to the TLR5 stimulation with an increased expression of IL-6 and reduced level of active TGFβ. The observed changes in the conjunctiva after flagellin application correspond with the development of clinical signs of chronic ocular mucosal inflammation including corneal epitheliopathy. Collectively, these findings demonstrate the ability of ocular mucosal dendritic cells to extend trans-epithelial dendrites in response to increased microbial colonization at the ocular surface. Moreover, this study provides key insight into how goblet cell responses to microbial stimuli may contribute to the disruption of ocular mucosal homeostasis and chronic ocular mucosal inflammation." @default.
• W3188373912 created "2021-08-16" @default.
• W3188373912 creator A5067213910 @default.
• W3188373912 creator A5087432027 @default.
• W3188373912 creator A5090186976 @default.
• W3188373912 date "2021-08-09" @default.
• W3188373912 modified "2023-09-27" @default.
• W3188373912 title "Conjunctival Goblet Cell Responses to TLR5 Engagement Promote Activation of Local Antigen-Presenting Cells" @default.
• W3188373912 cites W1575754639 @default.
• W3188373912 cites W1677986074 @default.
• W3188373912 cites W1845924540 @default.
• W3188373912 cites W1855051021 @default.
• W3188373912 cites W1944941250 @default.
• W3188373912 cites W1978045632 @default.
• W3188373912 cites W1978475186 @default.
• W3188373912 cites W1982936044 @default.
• W3188373912 cites W2000961556 @default.
• W3188373912 cites W2009112167 @default.
• W3188373912 cites W2019205435 @default.
• W3188373912 cites W2021920348 @default.
• W3188373912 cites W2061845364 @default.
• W3188373912 cites W2084853180 @default.
• W3188373912 cites W2088339251 @default.
• W3188373912 cites W2088553840 @default.
• W3188373912 cites W2099540110 @default.
• W3188373912 cites W2112421427 @default.
• W3188373912 cites W2118713387 @default.
• W3188373912 cites W2125061065 @default.
• W3188373912 cites W2152168515 @default.
• W3188373912 cites W2171980652 @default.
• W3188373912 cites W2195529641 @default.
• W3188373912 cites W2342389707 @default.
• W3188373912 cites W2770292471 @default.
• W3188373912 cites W2787592352 @default.
• W3188373912 cites W2806840045 @default.
• W3188373912 cites W2843305576 @default.
• W3188373912 cites W2891617094 @default.
• W3188373912 doi "https://doi.org/10.3389/fimmu.2021.716939" @default.
• W3188373912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8380822" @default.
• W3188373912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34434198" @default.
• W3188373912 hasPublicationYear "2021" @default.
• W3188373912 type Work @default.
• W3188373912 sameAs 3188373912 @default.
• W3188373912 citedByCount "4" @default.
• W3188373912 countsByYear W31883739122021 @default.
• W3188373912 countsByYear W31883739122022 @default.
• W3188373912 countsByYear W31883739122023 @default.
• W3188373912 crossrefType "journal-article" @default.
• W3188373912 hasAuthorship W3188373912A5067213910 @default.
• W3188373912 hasAuthorship W3188373912A5087432027 @default.
• W3188373912 hasAuthorship W3188373912A5090186976 @default.
• W3188373912 hasBestOaLocation W31883739121 @default.
• W3188373912 hasConcept C104317684 @default.
• W3188373912 hasConcept C127716648 @default.
• W3188373912 hasConcept C136449434 @default.
• W3188373912 hasConcept C142724271 @default.
• W3188373912 hasConcept C16930146 @default.
• W3188373912 hasConcept C170493617 @default.
• W3188373912 hasConcept C203014093 @default.
• W3188373912 hasConcept C2775941134 @default.
• W3188373912 hasConcept C2775984611 @default.
• W3188373912 hasConcept C2776090121 @default.
• W3188373912 hasConcept C2776709828 @default.
• W3188373912 hasConcept C2777338127 @default.
• W3188373912 hasConcept C2778170410 @default.
• W3188373912 hasConcept C2779919695 @default.
• W3188373912 hasConcept C38117629 @default.
• W3188373912 hasConcept C502942594 @default.
• W3188373912 hasConcept C529295009 @default.
• W3188373912 hasConcept C55493867 @default.
• W3188373912 hasConcept C67662055 @default.
• W3188373912 hasConcept C71924100 @default.
• W3188373912 hasConcept C86803240 @default.
• W3188373912 hasConcept C8891405 @default.
• W3188373912 hasConcept C95444343 @default.
• W3188373912 hasConceptScore W3188373912C104317684 @default.
• W3188373912 hasConceptScore W3188373912C127716648 @default.
• W3188373912 hasConceptScore W3188373912C136449434 @default.
• W3188373912 hasConceptScore W3188373912C142724271 @default.
• W3188373912 hasConceptScore W3188373912C16930146 @default.
• W3188373912 hasConceptScore W3188373912C170493617 @default.
• W3188373912 hasConceptScore W3188373912C203014093 @default.
• W3188373912 hasConceptScore W3188373912C2775941134 @default.
• W3188373912 hasConceptScore W3188373912C2775984611 @default.
• W3188373912 hasConceptScore W3188373912C2776090121 @default.
• W3188373912 hasConceptScore W3188373912C2776709828 @default.
• W3188373912 hasConceptScore W3188373912C2777338127 @default.
• W3188373912 hasConceptScore W3188373912C2778170410 @default.
• W3188373912 hasConceptScore W3188373912C2779919695 @default.
• W3188373912 hasConceptScore W3188373912C38117629 @default.
• W3188373912 hasConceptScore W3188373912C502942594 @default.
• W3188373912 hasConceptScore W3188373912C529295009 @default.
• W3188373912 hasConceptScore W3188373912C55493867 @default.
• W3188373912 hasConceptScore W3188373912C67662055 @default.
• W3188373912 hasConceptScore W3188373912C71924100 @default.
• W3188373912 hasConceptScore W3188373912C86803240 @default.
• W3188373912 hasConceptScore W3188373912C8891405 @default.
• W3188373912 hasConceptScore W3188373912C95444343 @default.
• W3188373912 hasFunder F4320310048 @default.
• W3188373912 hasFunder F4320337350 @default.

• next