Matches in SemOpenAlex for { <https://semopenalex.org/work/W3188651624> ?p ?o ?g. }
- W3188651624 endingPage "619" @default.
- W3188651624 startingPage "619" @default.
- W3188651624 abstract "We sought to characterize the lipid profile of skeletal muscle cell-derived Extracellular Vesicles (EVs) to determine if a hypertrophic stimulus would affect the lipid composition of C2C12 myotube-derived EVs. Analyses included C2C12 murine myoblasts differentiated into myotubes and treated with Insulin-Like Growth Factor 1 (IGF-1) for 24 h to induce hypertrophic growth. EVs were isolated from cell culture media, quantified using Nanoparticle Tracking Analysis (NTA) and analyzed using Transmission Electron Microscopy (TEM). EVs were homogenized and lipids extracted for quantification by Mass Spectrometry followed by downstream lipid class enrichment and lipid chain analysis. IGF-1 treatment elicited an increase in CD63 and CD81 levels (39% and 21%) compared to the controls (16%), respectively. Analysis revealed that skeletal muscle-derived EVs are enriched in bioactive lipids that are likely selectively incorporated into EVs during hypertrophic growth. IGF-1 treatment of myotubes had a significant impact on the levels of diacylglycerol (DG) and ceramide (Cer) in secreted EVs. Specifically, the proportion of unsaturated DG was two- to three-fold higher in EVs derived from IGF-treated cells, as compared to those from control cells. The levels of saturated DG were unaffected. Selective increases were similarly seen in C16- and C24-Cer but not in other species. Levels of free sphingoid bases tended to decrease, while those of sphingosine-1-phosphate was unaffected. Our results suggest that the lipid composition and biogenesis of skeletal muscle-derived EVs, are specific and highly selective during hypertrophic growth." @default.
- W3188651624 created "2021-08-16" @default.
- W3188651624 creator A5028728729 @default.
- W3188651624 creator A5029596491 @default.
- W3188651624 creator A5036185563 @default.
- W3188651624 creator A5066374603 @default.
- W3188651624 creator A5073816175 @default.
- W3188651624 date "2021-08-12" @default.
- W3188651624 modified "2023-09-26" @default.
- W3188651624 title "Skeletal Muscle Cell Growth Alters the Lipid Composition of Extracellular Vesicles" @default.
- W3188651624 cites W1571387405 @default.
- W3188651624 cites W1877686344 @default.
- W3188651624 cites W1914452730 @default.
- W3188651624 cites W1981777743 @default.
- W3188651624 cites W1993778451 @default.
- W3188651624 cites W2007652184 @default.
- W3188651624 cites W2019507092 @default.
- W3188651624 cites W2019917125 @default.
- W3188651624 cites W2027203412 @default.
- W3188651624 cites W2036841744 @default.
- W3188651624 cites W2037773086 @default.
- W3188651624 cites W2047785960 @default.
- W3188651624 cites W2049069193 @default.
- W3188651624 cites W2055313665 @default.
- W3188651624 cites W2055345515 @default.
- W3188651624 cites W2072063027 @default.
- W3188651624 cites W2072167135 @default.
- W3188651624 cites W2073341747 @default.
- W3188651624 cites W2075286695 @default.
- W3188651624 cites W2076315676 @default.
- W3188651624 cites W2081098333 @default.
- W3188651624 cites W2083637865 @default.
- W3188651624 cites W2086275775 @default.
- W3188651624 cites W2089154872 @default.
- W3188651624 cites W2095038001 @default.
- W3188651624 cites W2099491642 @default.
- W3188651624 cites W2103361158 @default.
- W3188651624 cites W2111560326 @default.
- W3188651624 cites W2113441355 @default.
- W3188651624 cites W2119043140 @default.
- W3188651624 cites W2119764013 @default.
- W3188651624 cites W2120052164 @default.
- W3188651624 cites W2128443570 @default.
- W3188651624 cites W2131083445 @default.
- W3188651624 cites W2140362890 @default.
- W3188651624 cites W2143145944 @default.
- W3188651624 cites W2163643821 @default.
- W3188651624 cites W2168020094 @default.
- W3188651624 cites W2254934739 @default.
- W3188651624 cites W2281134225 @default.
- W3188651624 cites W2306506161 @default.
- W3188651624 cites W2323793261 @default.
- W3188651624 cites W2523456649 @default.
- W3188651624 cites W2552505527 @default.
- W3188651624 cites W2557552778 @default.
- W3188651624 cites W2582091628 @default.
- W3188651624 cites W2600094664 @default.
- W3188651624 cites W2616431713 @default.
- W3188651624 cites W2706098188 @default.
- W3188651624 cites W2751240956 @default.
- W3188651624 cites W2771692071 @default.
- W3188651624 cites W2789596991 @default.
- W3188651624 cites W2789976756 @default.
- W3188651624 cites W2790979914 @default.
- W3188651624 cites W2799875505 @default.
- W3188651624 cites W2804173945 @default.
- W3188651624 cites W2807492713 @default.
- W3188651624 cites W2888695934 @default.
- W3188651624 cites W2889670640 @default.
- W3188651624 cites W2890739664 @default.
- W3188651624 cites W2894800084 @default.
- W3188651624 cites W2900756811 @default.
- W3188651624 cites W2908927563 @default.
- W3188651624 cites W2912631113 @default.
- W3188651624 cites W2913009425 @default.
- W3188651624 cites W2931418311 @default.
- W3188651624 cites W2939992495 @default.
- W3188651624 cites W2981562683 @default.
- W3188651624 cites W2988756053 @default.
- W3188651624 cites W2999852770 @default.
- W3188651624 cites W3005642548 @default.
- W3188651624 cites W3007473171 @default.
- W3188651624 cites W3010132504 @default.
- W3188651624 cites W3011287221 @default.
- W3188651624 cites W3040772304 @default.
- W3188651624 cites W3091856220 @default.
- W3188651624 cites W3094881473 @default.
- W3188651624 cites W3134302489 @default.
- W3188651624 cites W3164680687 @default.
- W3188651624 cites W3185779416 @default.
- W3188651624 doi "https://doi.org/10.3390/membranes11080619" @default.
- W3188651624 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8397976" @default.
- W3188651624 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34436382" @default.
- W3188651624 hasPublicationYear "2021" @default.
- W3188651624 type Work @default.
- W3188651624 sameAs 3188651624 @default.
- W3188651624 citedByCount "7" @default.
- W3188651624 countsByYear W31886516242022 @default.